Investigations on Antithrombin III in Normal Plasma and Serum

Summary. Studies on antithrombin III (AT‐III) were made by a modification of the two dimensional crossed immunoelectrophoresis technique and gel filtration. Mixing various quantities of heparin with agarose in the first phase of electro‐phoresis, AT‐III from normal human plasma and serum revealed a heterogeneity which depended on the heparin concentration in the agarose gel. At heparin concentrations higher than 16 u/ml, AT‐III displayed three components with different electrophoretic mobilities. The component with the highest mobility (designated immunoantithrombin III1: IAT‐III1) dominated in plasma. In normal serum, however, the quantity of this component was decreased and the two other peaks with a slower electrophoretic mobility (IAT‐III2 and IAT‐III3) became more evident.

[1]  D. Pepper,et al.  Plasma and serum antithrombin - III: differentiation by crossed immunoelectrophoresis. , 1975, Thrombosis research.

[2]  P. Desimone,et al.  Familial thrombosis due to antithrombin 3 deficiency. , 1974, Blood.

[3]  R. Rosenberg,et al.  Anticoagulant Action of Heparin , 1973, Nature.

[4]  B. Binder.  On the complex formation of antithrombin 3 with thrombin. Gelfiltration studies on human plasma and serum. , 1973, Thrombosis et diathesis haemorrhagica.

[5]  J. van der Meer,et al.  Antithrombin-III deficiency in a Dutch family , 1973 .

[6]  W. Seegers,et al.  Inhibition of thrombin and autoprothrombin C (F-X a ) as a mutual depletion system. , 1971, Thrombosis et diathesis haemorrhagica.

[7]  S. Wessler,et al.  Identity of plasma-activated factor X inhibitor with antithrombin 3 and heparin cofactor. , 1971, The Journal of biological chemistry.

[8]  W. Seegers,et al.  Antithrombin: Inhibition of Thrombin and Autoprothrombin C (F-Xa) as a Mutual Depletion System , 1971 .

[9]  R. Biggs,et al.  Antithrombin III, Antifactor Xa and Heparin , 1970 .

[10]  U. Abildgaard.  Binding of thrombin to antithrombin III. , 1969, Scandinavian journal of clinical and laboratory investigation.

[11]  C. Laurell,et al.  Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. , 1966, Analytical biochemistry.

[12]  O. Egeberg.  Inherited Antithrombin Deficiency Causing Thrombophilia , 1965, Thrombosis and Haemostasis.

[13]  C. Laurell,et al.  ANTIGEN-ANTIBODY CROSSED ELECTROPHORESIS. , 1965, Analytical biochemistry.

[14]  T. Astrup,et al.  The biological significance of fibrinolysis. , 1956, Lancet.