Construction of Large-Volume Tissue Mimics with 3D Functional Vascular Networks

We used indirect stereolithography (SL) to form inner-layered fluidic networks in a porous scaffold by introducing a hydrogel barrier on the luminal surface, then seeded the networks separately with human umbilical vein endothelial cells and human lung fibroblasts to form a tissue mimic containing vascular networks. The artificial vascular networks provided channels for oxygen transport, thus reducing the hypoxic volume and preventing cell death. The endothelium of the vascular networks significantly retarded the occlusion of channels during whole-blood circulation. The tissue mimics have the potential to be used as an in vitro platform to examine the physiologic and pathologic phenomena through vascular architecture.

[1]  Milica Radisic,et al.  Perfusable branching microvessel bed for vascularization of engineered tissues , 2012, Proceedings of the National Academy of Sciences.

[2]  C. D. Murray,et al.  The Physiological Principle of Minimum Work: II. Oxygen Exchange in Capillaries. , 1926, Proceedings of the National Academy of Sciences of the United States of America.

[3]  Ying Zheng,et al.  In vitro microvessels for the study of angiogenesis and thrombosis , 2012, Proceedings of the National Academy of Sciences.

[4]  Bum Jin Kim,et al.  In vivo endothelization of tubular vascular grafts through in situ recruitment of endothelial and endothelial progenitor cells by RGD-fused mussel adhesive proteins , 2015, Biofabrication.

[5]  P. Vermette,et al.  Scaffold vascularization: a challenge for three-dimensional tissue engineering. , 2010, Current medicinal chemistry.

[6]  G. V. van Osch,et al.  Vascularization of prevascularized and non‐prevascularized fibrin‐based human adipose tissue constructs after implantation in nude mice , 2012, Journal of tissue engineering and regenerative medicine.

[7]  Vittorio Cristini,et al.  Analysis of cell growth in three-dimensional scaffolds. , 2006, Tissue engineering.

[8]  E. Dejana,et al.  Perspectives Series: Cell Adhesion in Vascular Biology Endothelial Adherens Junctions: Implications in the Control of Vascular Permeability and Angiogenesis , 1996 .

[9]  Dong-Woo Cho,et al.  Solid freeform fabrication technology applied to tissue engineering with various biomaterials , 2012 .

[10]  M. Toner,et al.  Oxygen uptake rates and liver‐specific functions of hepatocyte and 3T3 fibroblast co‐cultures , 2007, Biotechnology and bioengineering.

[11]  Philipp Jungebluth,et al.  The first tissue-engineered airway transplantation: 5-year follow-up results , 2014, The Lancet.

[12]  James J. Yoo,et al.  Tissue-engineered autologous bladders for patients needing cystoplasty , 2006, The Lancet.

[13]  A. Khademhosseini,et al.  Building Vascular Networks , 2012, Science Translational Medicine.

[14]  Ying Zheng,et al.  Formation of microvascular networks in vitro , 2013, Nature Protocols.

[15]  Brendon M. Baker,et al.  Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues , 2012 .

[16]  James J. Yoo,et al.  The realistic prediction of oxygen transport in a tissue-engineered scaffold by introducing time-varying effective diffusion coefficients. , 2011, Acta biomaterialia.

[17]  Guerlain Ulysse,et al.  Effect of Air Exposure and Suction on Blood Cell Activation and Hemolysis in an In Vitro Cardiotomy Suction Model , 2013, ASAIO journal.

[18]  Robert Liska,et al.  Water-soluble photopolymers for rapid prototyping of cellular materials , 2005 .

[19]  Hiroshi Yagi,et al.  Organ reengineering through development of a transplantable recellularized liver graft using decellularized liver matrix , 2010, Nature Medicine.

[20]  J. Tramper,et al.  Oxygen gradients in tissue‐engineered Pegt/Pbt cartilaginous constructs: Measurement and modeling , 2004, Biotechnology and bioengineering.

[21]  Chu Zhang,et al.  Biofunctionalization of electrospun PCL-based scaffolds with perlecan domain IV peptide to create a 3-D pharmacokinetic cancer model. , 2010, Biomaterials.

[22]  Armelle Le Guelte,et al.  Role of endothelial cell-cell junctions in endothelial permeability. , 2011, Methods in molecular biology.

[23]  E. Wall,et al.  The role of hypoxia inducible factor 1 (HIF-1) in hypoxia induced apoptosis , 2004, Journal of Clinical Pathology.

[24]  Sheila MacNeil,et al.  Progress and opportunities for tissue-engineered skin , 2007, Nature.

[25]  Bo Nilsson,et al.  Tracheobronchial transplantation with a stem-cell-seeded bioartificial nanocomposite: a proof-of-concept study , 2011 .

[26]  James J. Yoo,et al.  Design and assessment of a microfluidic network system for oxygen transport in engineered tissue. , 2013, Langmuir : the ACS journal of surfaces and colloids.

[27]  Toshiki Niino,et al.  Avidin-biotin binding-based cell seeding and perfusion culture of liver-derived cells in a porous scaffold with a three-dimensional interconnected flow-channel network. , 2007, Biomaterials.

[28]  Yasuyuki Sakai,et al.  A novel poly-L-lactic acid scaffold that possesses a macroporous structure and a branching/joining three-dimensional flow channel network : its fabrication and application to perfusion culture of human hepatoma Hep G2 cells , 2004 .

[29]  Cato T Laurencin,et al.  Induction of angiogenesis in tissue-engineered scaffolds designed for bone repair: A combined gene therapy–cell transplantation approach , 2008, Proceedings of the National Academy of Sciences.

[30]  Ashok Kumar,et al.  Skin tissue engineering for tissue repair and regeneration. , 2008, Tissue engineering. Part B, Reviews.

[31]  R. Reis,et al.  Surface controlled biomimetic coating of polycaprolactone nanofiber meshes to be used as bone extracellular matrix analogues , 2008, Journal of biomaterials science. Polymer edition.

[32]  Dong-Woo Cho,et al.  Enhanced endothelialization for developing artificial vascular networks with a natural vessel mimicking the luminal surface in scaffolds. , 2013, Acta biomaterialia.

[33]  Arnan Mitchell,et al.  A shear gradient–dependent platelet aggregation mechanism drives thrombus formation , 2009, Nature Medicine.

[34]  Dong-Woo Cho,et al.  Development of an indirect stereolithography technology for scaffold fabrication with a wide range of biomaterial selectivity. , 2012, Tissue engineering. Part C, Methods.

[35]  H N Sabbah,et al.  Measured Turbulence and Its Effect on Thrombus Formation , 1974, Circulation research.

[36]  Erik K. Bassett,et al.  Lung assist device technology with physiologic blood flow developed on a tissue engineered scaffold platform. , 2011, Lab on a chip.

[37]  Anthony Atala,et al.  Tissue engineering of human bladder. , 2011, British medical bulletin.

[38]  Dong-Woo Cho,et al.  Projection image-generation algorithm for fabrication of a complex structure using projection-based microstereolithography , 2012 .

[39]  Mitsuo Umezu,et al.  In Vitro Engineering of Vascularized Tissue Surrogates , 2013, Scientific Reports.

[40]  Harihara Baskaran,et al.  A rapid seeding technique for the assembly of large cell/scaffold composite constructs. , 2006, Tissue engineering.