Foreign protein production in plant tissue cultures.

Foreign proteins synthesised by plants are now in the marketplace, and clinical trials for plant-derived therapeutic proteins are underway. Economic analysis of plant production systems has helped identify the types of protein that would be most suitable for manufacture using tissue culture methods. The major advantages associated with in vitro plant systems include the ability to manipulate environmental conditions for better control over protein levels and quality, the rapidity of production compared with agriculture, and the use of simpler and cheaper downstream processing schemes for product recovery from the culture medium.

[1]  R Fischer,et al.  Expression and characterization of bispecific single-chain Fv fragments produced in transgenic plants. , 1999, European journal of biochemistry.

[2]  N. Emans,et al.  Towards molecular farming in the future: using plant‐cell‐suspension cultures as bioreactors , 1999, Biotechnology and applied biochemistry.

[3]  A. Kermode.  Mechanisms of Intracellular Protein Transport and Targeting in Plant Cells , 1996 .

[4]  P. Zambryski,et al.  Plasmodesmata signaling: many roles, sophisticated statutes. , 1999, Current opinion in plant biology.

[5]  R. Ferl,et al.  Processing of preproricin in transgenic tobacco. , 1999, Protein expression and purification.

[6]  C. Kado,et al.  Light-controlled expression of a foreign gene using the chalcone synthase promoter in tobacco BY-2 cells , 1998 .

[7]  I. Raskin,et al.  Production of recombinant proteins in plant root exudates , 1999, Nature Biotechnology.

[8]  E. Hood,et al.  Plant-based production of xenogenic proteins. , 1999, Current opinion in biotechnology.

[9]  L. Miele.  Plants as bioreactors for biopharmaceuticals: regulatory considerations. , 1997, Trends in biotechnology.

[10]  M. Figlerowicz,et al.  A plant‐derived edible vaccine against hepatitis B virus , 1999, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[11]  J. Ma,et al.  Plant expression systems for the production of vaccines. , 1999, Current topics in microbiology and immunology.

[12]  R. Evangelista,et al.  Processing of transgenic corn seed and its effect on the recovery of recombinant beta-glucuronidase. , 1998, Biotechnology and bioengineering.

[13]  R Fischer,et al.  Affinity-purification of a TMV-specific recombinant full-size antibody from a transgenic tobacco suspension culture. , 1999, Journal of immunological methods.

[14]  R. Bollini,et al.  Effects of N-glycosylation on the folding and structure of plant proteins , 1998 .

[15]  T. Lehner,et al.  Characterization of a recombinant plant monoclonal secretory antibody and preventive immunotherapy in humans , 1998, Nature Medicine.

[16]  James M. Lee,et al.  Effect of culture conditions on monoclonal antibody production from genetically modified tobacco suspension cultures , 1999 .

[17]  J. Kraehenbuhl,et al.  Defense of Mucosal Surfaces: Pathogenesis, Immunity and Vaccines , 1999, Current Topics in Microbiology and Immunology.

[18]  J. Boothe,et al.  Transgenic plants for therapeutic proteins: linking upstream and downstream strategies. , 1999, Current topics in microbiology and immunology.

[19]  P. Doran,et al.  Effect of bacitracin on growth and monoclonal antibody production by tobacco hairy roots and cell suspensions , 1999 .

[20]  V. Gomord,et al.  Expression of a carrot invertase gene in tobacco suspension cells cultivated in batch and continuous culture conditions. , 1999, Journal of bioscience and bioengineering.

[21]  U. Conrad,et al.  High-level and stable accumulation of single-chain Fv antibodies in plant storage organs , 1998 .

[22]  R. Reeves,et al.  Secretion of biologically active human interleukin-2 and interleukin-4 from genetically modified tobacco cells in suspension culture. , 1998, Protein expression and purification.

[23]  P. Doran,et al.  Production of monoclonal antibodies by tobacco hairy roots. , 1997, Biotechnology and bioengineering.

[24]  M. Levine,et al.  Immunogenicity in humans of a recombinant bacterial antigen delivered in a transgenic potato , 1998, Nature Medicine.

[25]  H. P. Fell,et al.  Expression and characterization of bryodin 1 and a bryodin 1-based single-chain immunotoxin from tobacco cell culture. , 1997, Bioconjugate chemistry.

[26]  P. Lerouge,et al.  N-Glycosylation of a mouse IgG expressed in transgenic tobacco plants. , 1999, Glycobiology.

[27]  R. Rodriguez,et al.  Production of functional human α1-antitrypsin by plant cell culture , 1999, Applied Microbiology and Biotechnology.

[28]  G. Allard,et al.  Production of a diagnostic monoclonal antibody in perennial alfalfa plants. , 1999, Biotechnology and bioengineering.

[29]  A. Hiatt,et al.  Production of antibodies in transgenic plants , 1989, Nature.

[30]  D. J. Lewandowski,et al.  Heterologous sequences greatly affect foreign gene expression in tobacco mosaic virus-based vectors. , 1999, Virology.

[31]  D A Russell,et al.  Feasibility of antibody production in plants for human therapeutic use. , 1999, Current topics in microbiology and immunology.

[32]  P. McGarvey,et al.  Plant biotechnology : new products and applications , 1999 .

[33]  R. Jefferis,et al.  Glycosylation of antibody molecules: structural and functional significance. , 1996, Chemical immunology.

[34]  T. Higgins,et al.  Engineering plant protein composition for improved nutrition , 1998 .

[35]  R. Evangelista,et al.  Process and Economic Evaluation of the Extraction and Purification of Recombinant β‐Glucuronidase from Transgenic Corn , 1998, Biotechnology progress.

[36]  U. Sonnewald,et al.  Production of new/modified proteins in transgenic plants. , 1999, Current opinion in biotechnology.

[37]  R. Levy,et al.  Rapid production of specific vaccines for lymphoma by expression of the tumor-derived single-chain Fv epitopes in tobacco plants. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[38]  M. Terashima,et al.  Effect of osmotic pressure on human α1-antitrypsin production by plant cell culture , 1999 .

[39]  J. Howard,et al.  Production of recombinant proteins in transgenic plants: Practical considerations , 1997, Biotechnology and bioengineering.

[40]  G. García-Casado,et al.  Role of complex asparagine-linked glycans in the allergenicity of plant glycoproteins. , 1996, Glycobiology.

[41]  S L Morrison,et al.  Effect of glycosylation on antibody function: implications for genetic engineering. , 1997, Trends in biotechnology.

[42]  D. Klessig,et al.  Engineering disease and pest resistance in plants. , 1998, Trends in microbiology.

[43]  T. Turpen.  Tobacco mosaic virus and the virescence of biotechnology. , 1999, Philosophical transactions of the Royal Society of London. Series B, Biological sciences.