Origin of the direct hippocampus-anterior thalamic bundle in the rat: A combined horseradish peroxidase-Golgi analysis

Abstract The origin of the direct fiber pathway from the hippocampus to the anterior thalamus was examined with the horseradish peroxidase technique and the resultant labeled neurons were compared with neurons impregnated by the Golgi technique. Injections of horseradish peroxidase into the anterior thalamus resulted in uptake of the compound by specialized areas of the subiculum and presubiculum. Labeled neurons in the subiculum were restricted to regions of subiculum distal to field CA1 and to cell laminae adjacent to the alveus. Presubicular labeled neurons were restricted to lamina principalis interna. The labeled neurons were characterized by exact position, dendritic orientation, and soma shape. Similarly appearing and located neurons were found in Golgi preparations. Based on an analysis of these types of neurons in the Golgi material, possible reciprocal connections between hippocampus and anterior thalamus are discussed.

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