Cloning and characterization of the low-affinity cyclic AMP phosphodiesterase gene of Saccharomyces cerevisiae

Saccharomyces cerevisiae contains two genes which encode cyclic AMP (cAMP) phosphodiesterase. We previously isolated and characterized PDE2, which encodes a high-affinity cAMP phosphodiesterase. We have now isolated the PDE1 gene of S. cerevisiae, which encodes a low-affinity cAMP phosphodiesterase. These two genes represent highly divergent branches in the evolution of phosphodiesterases. High-copy-number plasmids containing either PDE1 or PDE2 can reverse the growth arrest defects of yeast cells carrying the RAS2(Val-19) mutation. PDE1 and PDE2 appear to account for the aggregate cAMP phosphodiesterase activity of S. cerevisiae. Disruption of both PDE genes results in a phenotype which resembles that induced by the RAS2(Val-19) mutation. pde1- pde2- ras1- ras2- cells are viable.

[1]  Michael Wigler,et al.  Three different genes in S. cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase , 1987, Cell.

[2]  M. Wigler,et al.  Cloning and characterization of BCY1, a locus encoding a regulatory subunit of the cyclic AMP-dependent protein kinase in Saccharomyces cerevisiae , 1987, Molecular and cellular biology.

[3]  R. Kessin,et al.  Molecular cloning and developmental expression of the cyclic nucleotide phosphodiesterase gene of Dictyostelium discoideum. , 1986, The Journal of biological chemistry.

[4]  J. Beavo,et al.  Identification of a conserved domain among cyclic nucleotide phosphodiesterases from diverse species. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[5]  R. Davis,et al.  Molecular analysis of cDNA clones and the corresponding genomic coding sequences of the Drosophila dunce+ gene, the structural gene for cAMP phosphodiesterase. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[6]  M. Wigler,et al.  DNA sequence and characterization of the S. cerevisiae gene encoding adenylate cyclase , 1985, Cell.

[7]  M. Wigler,et al.  Differential activation of yeast adenylate cyclase by wild type and mutant RAS proteins , 1985, Cell.

[8]  M. Wigler,et al.  In yeast, RAS proteins are controlling elements of adenylate cyclase , 1985, Cell.

[9]  M. Wigler,et al.  Functional homology of mammalian and yeast RAS genes , 1985, Cell.

[10]  J. Londesborough,et al.  Purification of intact and nicked forms of a zinc-containing, Mg2+-dependent, low Km cyclic AMP phosphodiesterase from bakers' yeast. , 1984, The Journal of biological chemistry.

[11]  E. Scolnick,et al.  Requirement of either of a pair of ras-related genes of Saccharomyces cerevisiae for spore viability , 1984, Nature.

[12]  M. Wigler,et al.  Genetic analysis of yeast RAS1 and RAS2 genes , 1984, Cell.

[13]  E. Scolnick,et al.  Nucleotide sequence of two rasH related-genes isolated from the yeast Saccharomyces cerevisiae. , 1984, Nucleic acids research.

[14]  M. Wigler,et al.  Genes in S. cerevisiae encoding proteins with domains homologous to the mammalian ras proteins , 1984, Cell.

[15]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[16]  K. Murata,et al.  Transformation of intact yeast cells treated with alkali cations. , 1984, Journal of bacteriology.

[17]  E. Scolnick,et al.  ras-Related gene sequences identified and isolated from Saccharomyces cerevisiae , 1983, Nature.

[18]  J. Messing,et al.  Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. , 1983, Gene.

[19]  G. Hong,et al.  Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[20]  K. Matsumoto,et al.  Characterization of a cyclic nucleotide phosphodiesterase-deficient mutant in yeast. , 1983, The Journal of biological chemistry.

[21]  J. Londesborough,et al.  The zinc-containing high Km cyclic nucleotide phosphodiesterase of bakers' yeast. , 1983, The Journal of biological chemistry.

[22]  K. Matsumoto,et al.  Cyclic AMP may not be involved in catabolite repression in Saccharomyes cerevisiae: evidence from mutants capable of utilizing it as an adenine source , 1982, Journal of bacteriology.

[23]  J. Hartley,et al.  Nucleotide sequence of the yeast plasmid , 1980, Nature.

[24]  S. Cohen,et al.  Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. , 1980, Journal of molecular biology.

[25]  K. Nasmyth,et al.  Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[26]  P. Chambon,et al.  Organisation and sequences at the 5′ end of a cloned complete ovalbumin gene , 1979, Nature.

[27]  M. Shoji,et al.  Cytidine 3':5'-monophosphate phosphodiesterase in mammalian tissues. Occurrence and biological involvement. , 1978, The Journal of biological chemistry.

[28]  J. Kuo,et al.  Differential effects of cyclic nucleotides and their analogs and various agents on cyclic GMP-specific and cyclic AMP-specific phosphodiesterases purified from guinea pig lung. , 1978, Biochemical pharmacology.

[29]  F. Sanger,et al.  DNA sequencing with chain-terminating inhibitors. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[30]  J. Carbon,et al.  Functional expression of cloned yeast DNA in Escherichia coli. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[31]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[32]  E. Southern.  Detection of specific sequences among DNA fragments separated by gel electrophoresis. , 1975, Journal of molecular biology.

[33]  T. Maniatis,et al.  Nucleotide sequence of the rightward operator of phage lambda. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[34]  M. M. Appleman,et al.  Multiple cyclic nucleotide phosphodiesterase activities from rat brain. , 1971, Biochemistry.

[35]  H. Boyer,et al.  A complementation analysis of the restriction and modification of DNA in Escherichia coli. , 1969, Journal of molecular biology.

[36]  V. Chester.  Heritable glycogen-storage deficiency in yeast and its induction by ultra-violet light. , 1968, Journal of general microbiology.

[37]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.