Gene expression of type I and type III collagen by mechanical stretch in anterior cruciate ligament cells.

Mechanical stretch affects the healing and remodeling process of the anterior cruciate ligament (ACL) after surgery in important ways. In this study, the effects of mechanical stress on gene expression of type I and III collagen by cultured human ACL cells and roles of transforming growth factor (TGF)-beta1 in the regulation of mechanical strain-induced gene expression were investigated. Uniaxial cyclic stretch was applied on ACL cells at 10 cycles/min with 10% length stretch for 24 h. mRNA expression of the type I and type III collagen was increased by the cyclic stretch. TGF-beta1 protein in the cell culture supernatant was also increased by the stretch. In the presence of anti-TGF-beta1 antibody, stretch-induced increase in type I and type III mRNA expression was markedly ablated. The results suggest that the stretch-induced mRNA expression of the type I and type III collagen is mediated via an autocrine mechanism of TGF-beta1 released from ligament cells.

[1]  Yasuteru Muragaki,et al.  Stretch-Induced Collagen Synthesis in Cultured Smooth Muscle Cells from Rabbit Aortic Media and a Possible Involvement of Angiotensin II and Transforming Growth Factor-β , 1998, Journal of Vascular Research.

[2]  K. Miyazono,et al.  Structure, Function and Possible Clinical Application of Transforming Growth Factor‐β , 1992 .

[3]  R. Hunter,et al.  The anterior cruciate ligament. , 2017 .

[4]  S. Woo,et al.  Effect of growth factors on matrix synthesis by ligament fibroblasts , 1997, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[5]  Richard T. Lee,et al.  Induction of Tenascin-C in Cardiac Myocytes by Mechanical Deformation , 1999, The Journal of Biological Chemistry.

[6]  H. Tohyama,et al.  Significance of graft tension in anterior cruciate ligament reconstruction Basic background and clinical outcome , 1998, Knee Surgery, Sports Traumatology, Arthroscopy.

[7]  Keiji Naruse,et al.  Involvement of SA channels in orienting response of cultured endothelial cells to cyclic stretch , 1998 .

[8]  M. Braddock,et al.  Fluid shear stress activation of egr-1 transcription in cultured human endothelial and epithelial cells is mediated via the extracellular signal-related kinase 1/2 mitogen-activated protein kinase pathway. , 1998, The Journal of clinical investigation.

[9]  W. Akeson,et al.  Time‐dependent increases in type‐III collagen gene expression in medial collateral ligament fibroblasts under cyclic strains , 2000, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[10]  M. O’Connor-McCourt,et al.  Characterization of recombinant soluble human transforming growth factor-β receptor Type II (rhTGF-βsRII) , 1995 .

[11]  C. Frank,et al.  Influence of exogenous growth factors on the synthesis and secretion of collagen types I and III by explants of normal and healing rabbit ligaments. , 1994, Biochemistry and cell biology = Biochimie et biologie cellulaire.

[12]  L. Yahia,et al.  Proliferative and matrix synthesis response of canine anterior cruciate ligament fibroblasts submitted to combined growth factors , 1996, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[13]  D Amiel,et al.  Tendons and ligaments: A morphological and biochemical comparison , 1984, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[14]  C. Hung,et al.  Intracellular calcium response of ACL and MCL ligament fibroblasts to fluid-induced shear stress. , 1997, Cellular signalling.

[15]  R. Brand,et al.  Cell alignment is induced by cyclic changes in cell length: studies of cells grown in cyclically stretched substrates , 2001, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[16]  D. Amiel,et al.  Differential metabolic responses of periarticular ligaments and tendon to joint immobilization. , 1992, Journal of applied physiology.

[17]  K. Miyazono,et al.  Structure, function and possible clinical application of transforming growth factor-beta. , 1992, The Journal of dermatology.

[18]  W. Akeson,et al.  Decrease in fibronectin occurs coincident with the increased expression of its integrin receptor alpha5beta1 in stress-deprived ligaments. , 1997, The Iowa orthopaedic journal.

[19]  M. O’Connor-McCourt,et al.  Characterization of recombinant soluble human transforming growth factor-beta receptor type II (rhTGF-beta sRII). , 1995, Cytokine.

[20]  Christopher J. O’Callaghan,et al.  Mechanical Strain–Induced Extracellular Matrix Production by Human Vascular Smooth Muscle Cells: Role of TGF-&bgr;1 , 2000, Hypertension.

[21]  A. Ghosh,et al.  Antagonistic regulation of type I collagen gene expression by interferon-gamma and transforming growth factor-beta. Integration at the level of p300/CBP transcriptional coactivators. , 2001, The Journal of biological chemistry.

[22]  Hans Kresse,et al.  Proteoglycans of the extracellular matrix and growth control , 2001, Journal of cellular physiology.

[23]  T Delhaas,et al.  Differential responses of adult cardiac fibroblasts to in vitro biaxial strain patterns. , 1999, Journal of molecular and cellular cardiology.

[24]  Keiji Naruse,et al.  Up-regulation of COX2 expression by uni-axial cyclic stretch in human lung fibroblast cells. , 1998, Biochemical and biophysical research communications.

[25]  S. Maestrini,et al.  Mechanical stretch-induced fibronectin and transforming growth factor-beta1 production in human mesangial cells is p38 mitogen-activated protein kinase-dependent. , 2000, Diabetes.

[26]  W. Akeson,et al.  Characterization of the intrinsic properties of the anterior cruciate and medial collateral ligament cells: An in vitro cell culture study , 1992, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[27]  S. Woo,et al.  Tissue engineering of ligament and tendon healing. , 1999, Clinical orthopaedics and related research.

[28]  M. Kurosaka,et al.  Localization of growth factors in the reconstructed anterior cruciate ligament: immunohistological study in dogs , 2000, Knee Surgery, Sports Traumatology, Arthroscopy.

[29]  W. Akeson,et al.  The phenomenon of “Ligamentization”: Anterior cruciate ligament reconstruction with autogenous patellar tendon , 1986, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[30]  F. Girgis,et al.  The cruciate ligaments of the knee joint. Anatomical, functional and experimental analysis. , 1975, Clinical orthopaedics and related research.

[31]  N. Nakamura,et al.  Temporal and spatial expression of transforming growth factor‐β in the healing patellar ligament of the rat , 1997, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[32]  A. Amis,et al.  Functional anatomy of the anterior cruciate ligament. Fibre bundle actions related to ligament replacements and injuries. , 1991, The Journal of bone and joint surgery. British volume.

[33]  W. Akeson,et al.  Growth factor expression in healing rabbit medial collateral and anterior cruciate ligaments. , 1998, The Iowa orthopaedic journal.