Post-capillary fluorescence detection in capillary zone electrophoresis using o-phthaldialdehyde

Abstract A post-capillary fluorescence detection scheme for capillary zone electrophoresis is described using o-phthaldialdehyde (OPA) as the tagging reagent. Use of a coaxial capillary reactor affords mixing of the OPA reagent with migrating zones without excessive zone broadening. The detector is linear over three orders of magnitude and shows detection limits for amino acids and proteins in the femtogram (attomol) range.

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