Influence of extraction solvent system on extractability of lipid components from different microalgae species

Abstract The purpose of this work was to evaluate two food grade solvent systems, hexane/isopropanol (3:2; HI) and hexane (H), for the extraction of lipids from different omega-3 LC-PUFA rich microalgae: Isochrysis galbana, Nannochloropis gaditana, Nannochloropsis sp. and Phaeodactylum tricornutum. We not only focused on differences in lipid yield, but also lipid class, omega-3 LC-PUFA, carotenoid and sterol yields. Furthermore, an estimation of the feasibility of these microalgae oils as an alternative for fish oil was made. For all tested microalgae species, the highest food grade lipid, lipid class, omega-3 PUFA, carotenoid and sterol yield were obtained with HI, with a general recovery highest from Isochrysis, lowest from both Nannochloropsis species, and intermediate from Phaeodactylum. Total lipid recovery values between 14 and 76% depending on solvent and species were obtained. It was also shown that the omega-3 fatty acid content of all oils was quite similar, while only the H extract was enriched in neutral lipids. Carotenoids were co-extracted in a significant amount, although the content in the various oils was quite different.

[1]  Roberto Parra-Saldivar,et al.  Influence of extraction solvent system on the extractability of lipid components from the biomass of Nannochloropsis gaditana , 2013, Journal of Applied Phycology.

[2]  J. DeLany,et al.  New method for GC/FID and GC-C-IRMS analysis of plasma free fatty acid concentration and isotopic enrichment. , 2008, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[3]  M. Gordon,et al.  Dietary supplementation with a natural carotenoid mixture decreases oxidative stress , 2003, European Journal of Clinical Nutrition.

[4]  Uğur Gogus,et al.  N-3 Omega fatty acids: a review of current knowledge. , 2010 .

[5]  V. Martin‐Jézéquel,et al.  Surface Chemical Composition of Diatoms , 2009, Chembiochem : a European journal of chemical biology.

[6]  Imogen Foubert,et al.  Optimization of an Analytical Procedure for Extraction of Lipids from Microalgae , 2012 .

[7]  S. Derenne,et al.  Distribution of aliphatic, nonhydrolyzable biopolymers in marine microalgae , 1999 .

[8]  L. L. Jackson,et al.  Lipid sovent systems are not equivalent for analysis of lipid classes in the microeukaryotic green alga, Chlorella , 1988 .

[9]  Elizabeth J Johnson.  The role of carotenoids in human health. , 2002, Nutrition in clinical care : an official publication of Tufts University.

[10]  K. Dewettinck,et al.  Properties, analysis and purification of milk polar lipids , 2006 .

[11]  Imogen Foubert,et al.  Nutritional evaluation of microalgae oils rich in omega-3 long chain polyunsaturated fatty acids as an alternative for fish oil. , 2014, Food chemistry.

[12]  A. Solovchenko.  Physiological role of neutral lipid accumulation in eukaryotic microalgae under stresses , 2012, Russian Journal of Plant Physiology.

[13]  R. Russell,et al.  β-Carotene and Other Carotenoids as Antioxidants , 1999 .

[14]  J. Damsté,et al.  Resistant biomacromolecules in marine microalgae of the classes Eustigmatophyceae and Chlorophyceae: Geochemical implications , 1997 .

[15]  C. von Schacky,et al.  Incorporation of EPA and DHA into plasma phospholipids in response to different omega-3 fatty acid formulations - a comparative bioavailability study of fish oil vs. krill oil , 2011, Lipids in Health and Disease.

[16]  P. Webley,et al.  Extraction of oil from microalgae for biodiesel production: A review. , 2012, Biotechnology advances.

[17]  Z. Ramazanov,et al.  The effects of Xanthigen™ in the weight management of obese premenopausal women with non‐alcoholic fatty liver disease and normal liver fat , 2010, Diabetes, obesity & metabolism.

[18]  V. Piironen,et al.  Factors affecting sample preparation in the gas chromatographic determination of plant sterols in whole wheat flour , 2000 .

[19]  R. Balasubramanian,et al.  Factors affecting cellular lipid extraction from marine microalgae , 2013 .

[20]  B. Meyer,et al.  Are We Consuming Enough Long Chain Omega-3 Polyunsaturated Fatty Acids for Optimal Health? Are We Consuming Enough Long Chain Omega-3 Polyunsaturated Fatty Acids for Optimal Health? Are We Consuming Enough Long Chain Omega-3 Polyunsaturated Fatty Acids for Optimal Health? , 2022 .

[21]  K. Miyashita,et al.  Oxidative stability of glyceroglycolipids containing polyunsaturated fatty acids. , 2012, Journal of oleo science.

[22]  H. Oh,et al.  Rapid method for the determination of lipid from the green alga Botryococcus braunii , 1998 .

[23]  C. Billard,et al.  Sterolic biomarkers in marine phytoplankton. I. Free and conjugated sterols of Pavlova lutheri (Haptophyta) , 1996 .

[24]  C. Billard,et al.  STEROLIC BIOMARKERS IN MARINE PHYTOPLANKTON. II. FREE AND CONJUGATED STEROLS OF SEVEN SPECIES USED IN MARICULTURE , 1998 .

[25]  S. Abidi.  Capillary electrochromatography of sterols and related steryl esters derived from vegetable oils. , 2004, Journal of chromatography. A.

[26]  Yuan-Kun Lee,et al.  Determination of biomass dry weight of marine microalgae , 1997, Journal of Applied Phycology.

[27]  Z. Cohen,et al.  LC-PUFA from photosynthetic microalgae: occurrence, biosynthesis, and prospects in biotechnology , 2011, Applied Microbiology and Biotechnology.

[28]  Daniel J. Repeta,et al.  Improved HPLC method for the analysis of chlorophylls and carotenoids from marine phytoplankton , 1991 .

[29]  M. Øverland,et al.  Evaluation of microalgae as sources of digestible nutrients for monogastric animals , 2011 .

[30]  B. Patil,et al.  Effects of a Carrot Juice Intervention on Plasma Carotenoids, Oxidative Stress, and Inflammation in Overweight Breast Cancer Survivors , 2012, Nutrition and cancer.

[31]  T. Maoka,et al.  Suppressive effects of alloxanthin and diatoxanthin from Halocynthia roretzi on LPS-induced expression of pro-inflammatory genes in RAW264.7 cells. , 2008, Journal of oleo science.

[32]  M. Eeckhout,et al.  Influence of drying and storage on lipid and carotenoid stability of the microalga Phaeodactylum tricornutum. , 2011, Journal of agricultural and food chemistry.

[33]  C. Bruneel,et al.  Impact of feed supplementation with different omega-3 rich microalgae species on enrichment of eggs of laying hens. , 2013, Food chemistry.

[34]  E. Molina Grima,et al.  Comparison between extraction of lipids and fatty acids from microalgal biomass , 1994 .

[35]  W. Christie.  Lipid analysis;: Isolation, separation, identification, and structural analysis of lipids , 1973 .

[36]  D. H. Jones,et al.  Biochemical prey recognition by planktonic protozoa. , 2007, Environmental microbiology.

[37]  P. Adlercreutz,et al.  Monitoring the oxidation of docosahexaenoic acid in lipids , 2005, Lipids.

[38]  G. Britton.  Functions of Intact Carotenoids , 2008 .

[39]  E. A. Abdelkader,et al.  Mixed-Polarity Azeotropic Solvents for Efficient Extraction of Lipids from Nannochloropsis Microalgae , 2011 .

[40]  Marie A G Alminger,et al.  Effect of the consumption of a fruit and vegetable soup with high in vitro carotenoid bioaccessibility on serum carotenoid concentrations and markers of oxidative stress in young men , 2012, European Journal of Nutrition.

[41]  Stefan Schouten,et al.  Cell wall-specific ω-hydroxy fatty acids in some freshwater green microalgae , 1998 .