Soil Fungal Communities in Pioneer

Soil fungal community assembly is driven by deterministic and stochas16 tic processes. However, the contribution of these mechanisms to structure the com17 position of fungal communities of forest soils at the regional scale is poorly known. 18 Here, we investigate the relative importance of deterministic and stochastic processes 19 on fungal community composition by rDNA ITS metabarcoding in a Populus davidi20 ana pioneer forests along spatial-temporal gradients. We also assessed the impact 21 of elevation and seasonality. The soil fungal richness of P. davidiana pioneer forests 22 was significantly affected by elevation and less affected by season. Similarly, the vari23 ation in the fungal community composition according to the elevation was greater 24 than the effect of seasonality. The fungal community composition showed a signif25 icant distance-decay relationship. Variation partitioning analysis showed that plant 26 variables explained the soil fungal community variation. Through null model analysis, 27 we found that stochastic processes were dominant in the soil fungal community as28 sembly. However, the relative importance of ecological processes, including dispersal, 29 selection, and drift, was not consistent across the four soil fungal community assem30 blies. In addition, the undominated fraction (including weak selection, weak dispersal, 31 diversification and drift) had a high relative contribution to the soil fungal community 32 assembly process in the P. davidiana pioneer forest. In summary, our results demon33 strated that plant variables and the undominated fraction dominate the deterministic 34 and stochastic processes driving soil fungal community assemblies in a P. davidiana 35 pioneer forest at the regional scale, which provides new perspectives for the regional 36 scale studies of soil fungi. 37 IMPORTANCE Elevation and seasonality are important factors driving the composi38 tion of soil microbiota. Due to the tight interactions of soil fungi with their host trees in 39 forest ecosystems, the spatial variation of soil fungal community is often linked to the 40 variation in the composition of dominant tree species. We compared the responses of 41 soil fungal communities to seasonal and spatial changes at four levels in a temperate 42 poplar forest dominated by a single tree species under elevation changes. Elevation 43 had a higher impact than seasonality on the soil fungal beta diversity. Even when the 44 shift in dominant tree species was limited, vegetation factors still impact soil fungal 45 mS yste ms Sub mis sion Tem pla te mS yste ms Sub mis sion Tem pla te mS yste ms Sub mis sion Tem pla te mS yste ms Sub mis sion Tem pla te mS yste ms Sub mis sion Tem pla te mS yste ms Sub mis sion Tem pla te mS yste ms Sub mis sion Tem pla te mS yste ms Sub mis sion Tem pla te mS yste ms Sub mis sion Tem pla te mS yste ms Sub mis sion Tem pla te

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