Shape and Facet Analyses of Alveolar Airspaces of the Lung

Changes in lung volume during the breathing cycle and also lung diseases are likely to deform even the smallest airspace units, the alveoli. This study reports general ideas to investigate such changes with 3D digital image processing. It comprises morphological characterizations like volume and surface, an evaluation of the angle distribution between facets formed by the septal walls, the number of neighboring alveoli and a shape analysis of the alveolar airspace. The software used is open-source and custom programs are available at:

[1]  C. E. Perlman,et al.  Lung ventilation injures areas with discrete alveolar flooding, in a surface tension-dependent fashion. , 2014, Journal of applied physiology.

[2]  Automated quantitative 3D analysis of faceting of particles in tomographic datasets. , 2012, Ultramicroscopy.

[3]  Terry S. Yoo,et al.  Insight into Images: Principles and Practice for Segmentation, Registration, and Image Analysis , 2004 .

[4]  Max A. Viergever,et al.  elastix: A Toolbox for Intensity-Based Medical Image Registration , 2010, IEEE Transactions on Medical Imaging.

[5]  M. Ochs,et al.  Recent developments in 3-D reconstruction and stereology to study the pulmonary vasculature. , 2018, American journal of physiology. Lung cellular and molecular physiology.

[6]  Ole Tange,et al.  GNU Parallel: The Command-Line Power Tool , 2011, login Usenix Mag..

[7]  E. Hoffman,et al.  Assessment of morphometry of pulmonary acini in mouse lungs by nondestructive imaging using multiscale microcomputed tomography , 2012, Proceedings of the National Academy of Sciences.

[8]  Amy Henderson Squilacote.  The Paraview Guide , 2008 .

[9]  S. Schürch,et al.  Surface activity in situ, in vivo, and in the captive bubble surfactometer. , 2001, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology.

[10]  Christopher M Waters,et al.  What do we know about mechanical strain in lung alveoli? , 2011, American journal of physiology. Lung cellular and molecular physiology.

[11]  Matthias Ochs,et al.  The number of alveoli in the human lung. , 2004, American journal of respiratory and critical care medicine.

[12]  E. Weibel,et al.  American Thoracic Society Documents An Official Research Policy Statement of the American Thoracic Society/European Respiratory Society: Standards for Quantitative Assessment of Lung Structure , 2010 .

[13]  M. Ochs,et al.  Digital 3D reconstructions using histological serial sections of lung tissue including the alveolar capillary network. , 2017, American journal of physiology. Lung cellular and molecular physiology.

[14]  D. C. Sterio.  The unbiased estimation of number and sizes of arbitrary particles using the disector , 1984, Journal of microscopy.

[15]  H J Gundersen,et al.  Estimators of the number of objects per area unbiased by edge effects. , 1978, Microscopica acta.

[16]  Stefan Klein,et al.  Fast parallel image registration on CPU and GPU for diagnostic classification of Alzheimer's disease , 2013, Front. Neuroinform..

[17]  Bradford J. Smith,et al.  Alveolar derecruitment and collapse induration as crucial mechanisms in lung injury and fibrosis. , 2015, American journal of respiratory cell and molecular biology.

[18]  J. Crapo,et al.  Three-dimensional reconstruction of alveoli in the rat lung for pressure-volume relationships. , 1987, Journal of applied physiology.

[19]  E R Weibel,et al.  Morphometry of the human pulmonary acinus , 1988, The Anatomical record.

[20]  Marie-Françoise Devaux,et al.  COMPUTATION OF MINKOWSKI MEASURES ON 2D AND 3D BINARY IMAGES , 2011 .

[21]  A. Wright,et al.  Regional differences in alveolar density in the human lung are related to lung height. , 2015, Journal of applied physiology.

[22]  R. Harris,et al.  Stress concentration around an atelectatic region: A finite element model , 2014, Respiratory Physiology & Neurobiology.

[23]  J. Mead,et al.  Stress distribution in lungs: a model of pulmonary elasticity. , 1970, Journal of applied physiology.

[24]  William Schroeder,et al.  The Visualization Toolkit: An Object-Oriented Approach to 3-D Graphics , 1997 .

[25]  A. Kupsch,et al.  Quantitative structural assessment of heterogeneous catalysts by electron tomography. , 2011, Journal of the American Chemical Society.

[26]  J. Butler,et al.  Dihedral angles between alveolar septa. , 1988, Journal of applied physiology.

[27]  Stefan Klein,et al.  SimpleElastix: A User-Friendly, Multi-lingual Library for Medical Image Registration , 2016, 2016 IEEE Conference on Computer Vision and Pattern Recognition Workshops (CVPRW).

[28]  David Legland,et al.  Efficient N-Dimensional surface estimation using Crofton formula and run-length encoding , 2012, The Insight Journal.

[29]  M. Ochs,et al.  A Critical Comment on a Recent Publication Using Parenchymal Airspace Profiling. , 2017, American journal of respiratory cell and molecular biology.

[30]  J. Butler,et al.  Dihedral angles of septal "bend" structures in lung parenchyma. , 1996, Journal of applied physiology.

[31]  Ewald R Weibel,et al.  What makes a good lung? , 2009, Swiss medical weekly.