Prevalence and antimicrobial resistance of Salmonella isolated from poultry slaughterhouses in Korea.

We determined the antimicrobial resistance of Salmonella serovars from a total of 154 (44 chilling waters and 110 carcasses) samples collected from 22 poultry slaughterhouses. Standard culture techniques, Kauffmann-White slide agglutination and disc diffusion tests were used to isolate, and identify the serovars and to assess the antimicrobial activity, respectively. A total of 88 isolates belonging to 34 Salmonella serovars from 67 (43.5%) positive samples were identified. Among the samples examined, 68.2% (15/22), 22.7% (5/22), and 42.7% (47/110) from the first chilling waters, the last chilling waters, and carcasses were found contaminated with Salmonella, respectively. The prevalent serovars were S. Enteritidis (12.5%) followed by S. Montevideo and S. Senftenberg (8.0%). Rare Salmonella serovars such as S. Aba, S. Malmoe, S. Westhampton, S. Takoradi, and S. Baiboukoum in chicken slaughterhouses and S. Newbrunswick, S. Huddinge S. Glostrup, S. Dujugu, S. Goettingen and S. II in duck slaughterhouses were also detected. Among the serovars, 52.3% (46/88) and 21.6% (19/88) were resistant to one antibiotic and more than two antibiotics, respectively. High antimicrobial resistance rates against sulfamethoxazole (39.8%) followed by tetracycline (22.7%), nalidixic acid (21.6%), ampicillin and amoxicillin-clavulanic acid (8.0%), and chloramphenicol (4.5%) were observed. These results suggest more stringent hygienic measures should be taken to reduce the incidence of pathogen contamination in the food chain.

[1]  Jinyong Noh,et al.  Prevalence and antimicrobial resistance of Salmonella species isolated from chicken meats produced by different integrated broiler operations in Korea. , 2012, Poultry science.

[2]  F. Adzitey,et al.  Prevalence and antibiotic resistance of Salmonella serovars in ducks, duck rearing and processing environments in Penang, Malaysia , 2012 .

[3]  D. Rodrigues,et al.  Prevalence and antimicrobial resistance of Salmonella in chicken carcasses at retail in 15 Brazilian cities. , 2011, Revista panamericana de salud publica = Pan American journal of public health.

[4]  K. Seo,et al.  Prevalence, antibiotic resistance, and molecular characterization of Salmonella serovars in retail meat products. , 2011, Journal of food protection.

[5]  N. Ayaz,et al.  Serotype identification and antimicrobial resistance profiles of Salmonella spp. isolated from chicken carcasses , 2010, Tropical Animal Health and Production.

[6]  R. Capita,et al.  Prevalence of Salmonella enterica serovars and genovars from chicken carcasses in slaughterhouses in Spain , 2007, Journal of applied microbiology.

[7]  M. Maharjan,et al.  Prevalence of Salmonella Species in Various Raw Meat Samples of a Local Market in Kathmandu , 2006, Annals of the New York Academy of Sciences.

[8]  H. Tsen,et al.  Characterisation of antimicrobial resistance patterns and class 1 integrons among Escherichia coli and Salmonella enterica serovar Choleraesuis strains isolated from humans and swine in Taiwan. , 2006, International journal of antimicrobial agents.

[9]  J. Kaneene,et al.  Salmonella in food animals and humans in northern Thailand. , 2006, International journal of food microbiology.

[10]  J. S. Schrader,et al.  Prevalence of Campylobacter and Salmonella species on farm, after transport, and at processing in specialty market poultry. , 2006, Poultry science.

[11]  S. Choi,et al.  Increasing incidence of quinolone resistance in human non-typhoid Salmonella enterica isolates in Korea and mechanisms involved in quinolone resistance. , 2005, The Journal of antimicrobial chemotherapy.

[12]  S. Bloomfield,et al.  Contamination of chicken carcasses in Gauteng, South Africa, by Salmonella, Listeria monocytogenes and Campylobacter. , 2005, International journal of food microbiology.

[13]  H. Tsai,et al.  The prevalence and antimicrobial susceptibilities of Salmonella and Campylobacter in ducks in Taiwan. , 2005, The Journal of veterinary medical science.

[14]  Y. Chang,et al.  Prevalence and antibiotic susceptibility of Salmonella isolated from foods in Korea from 1993 to 2001. , 2003, Journal of food protection.

[15]  L. Peixe,et al.  Incidence of Salmonella from poultry products and their susceptibility to antimicrobial agents. , 2003, International journal of food microbiology.

[16]  T. Humphrey,et al.  Prevalence and numbers of Salmonella and Campylobacter spp. on raw, whole chickens in relation to sampling methods. , 2002, International journal of food microbiology.

[17]  K. Travers,et al.  Morbidity of infections caused by antimicrobial-resistant bacteria. , 2002, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[18]  J. Zumalacárregui,et al.  Prevalence of Salmonella and Campylobacter in retail chicken meat in Spain. , 2002, International journal of food microbiology.

[19]  D G White,et al.  The isolation of antibiotic-resistant salmonella from retail ground meats. , 2001, The New England journal of medicine.

[20]  G Rajashekara,et al.  Multidrug-resistant Salmonella Typhimurium DT104 in poultry. , 2000, Journal of food protection.

[21]  R. R. Henry.  Salmonella infection in ducks. , 2000 .

[22]  M. Popoff.  Antigenic formulas of the Salmonella serovars , 1997 .

[23]  S A McEwen,et al.  Amplification of an invA gene sequence of Salmonella typhimurium by polymerase chain reaction as a specific method of detection of Salmonella. , 1992, Molecular and cellular probes.

[24]  M. Colberg,et al.  Comparison of the DNA Probe to Culture Methods for the Detection of Salmonella on Poultry Carcasses and Processing Waters 1. , 1989, Journal of food protection.