ESTIMATION OF LENALIDOMIDE IN BULK AND ITS DOSAGE FORM USING UV SPECTROPHOTOMETRIC AND RP-HPLC METHODS

The present work includes a simple, economic, rapid, accurate and precise UV spectrophotometric and isocratic RP-HPLC method development for estimation of Lenalidomide in bulk and its formulation. Estimation was done at 250nm which was found to be λ max of Lenalidomide. The simple, selective, isocratic RP-HPLC method for Lenalidomide was developed on reverse phase C18 waters column (4.6 mm x 250 mm, 5μm ) with a mobile phase of acetate buffer (20 mM ) (pH 5): Methanol = 85:15 at a flow rate of 1ml/min and detection wavelength 250nm. The developed methods were validated successfully according to ICH Q2 (R1) guidelines. Both the spectrophotometric and chromatographic methods showed a