Factors related to contraction and mechanical strength of collagen gels seeded with canine endotenon cells.

Fibroblasts can construct a hydrated collagen lattice to a tissue-like structure that is greatly influenced by initial culture conditions. The purpose of this study was to investigate the effects of cell concentration and collagen concentration on the contraction kinetics and mechanical properties of resultant endotenon-derived fibroblast-seeded collagen lattice. The experiment was designed to evaluate the effect of cell concentration (0, 0.25, 0.5, and 1.0 x10(6) cells/mL) and collagen concentration (0.5, 1.0, 1.5, and 2.0 mg/mL). Collagen lattice contraction was recorded for 42 days, after which time the lattices were mechanically tested. The collagen lattices seeded with higher initial cell concentration had a shorter contraction lag phase (p < 0.01), and exhibited a higher ultimate stress (p < 0.01) and instantaneous and equilibrium modulus (p < 0.01) than those seeded with a lower initial cell concentration. The collagen lattices cultured with a lower initial collagen concentration also had a shorter contraction lag phase, and exhibited greater instantaneous and equilibrium modulus (p < 0.01) than those cultured with higher initial collagen concentration. The collagen lattices of initial 0.5 mg/mL collagen concentration had the highest value of ultimate stress (p < 0.03).

[1]  Albert K. Harris,et al.  Fibroblast traction as a mechanism for collagen morphogenesis , 1981, Nature.

[2]  K. Nakajima,et al.  Quantitative evaluation of the factors affecting the process of fibroblast-mediated collagen gel contraction by separating the process into three phases. , 1988, Collagen and related research.

[3]  D. Butler,et al.  In vitro characterization of mesenchymal stem cell-seeded collagen scaffolds for tendon repair: effects of initial seeding density on contraction kinetics. , 2000, Journal of biomedical materials research.

[4]  T. Tuan,et al.  In vitro fibroplasia: matrix contraction, cell growth, and collagen production of fibroblasts cultured in fibrin gels. , 1996, Experimental cell research.

[5]  Zhonggang Feng,et al.  Measurements of the mechanical properties of contracted collagen gels populated with rat fibroblasts or cardiomyocytes , 2003, Journal of Artificial Organs.

[6]  Raphael C. Lee,et al.  Mechanisms and dynamics of mechanical strengthening in ligament-equivalent fibroblast-populated collagen matrices , 1993, Annals of Biomedical Engineering.

[7]  D. Butler,et al.  Effects of cell seeding density and collagen concentration on contraction kinetics of mesenchymal stem cell-seeded collagen constructs. , 2006, Tissue engineering.

[8]  P. Rouxhet,et al.  Competitive adsorption of proteins: key of the relationship between substratum surface properties and adhesion of epithelial cells. , 1999, Biomaterials.

[9]  P. Agache,et al.  A new technique to study the mechanical properties of collagen lattices. , 1992, Journal of biomechanics.

[10]  T. Matsuda,et al.  Mechanical Stress-Induced Orientation and Ultrastructural Change of Smooth Muscle Cells Cultured in Three-Dimensional Collagen Lattices , 1994, Cell transplantation.

[11]  R T Tranquillo,et al.  Magnetically orientated tissue-equivalent tubes: application to a circumferentially orientated media-equivalent. , 1996, Biomaterials.

[12]  N. Occleston,et al.  Differences in proliferative rate and collagen lattice contraction between endotenon and synovial fibroblasts. , 1998, The Journal of hand surgery.

[13]  R. Zernicke,et al.  Mechanical load stimulates expression of novel genes in vivo and in vitro in avian flexor tendon cells. , 1999, Osteoarthritis and cartilage.

[14]  G. Michalopoulos,et al.  Collagen as a substrate for cell growth and differentiation. , 1982, Methods in enzymology.

[15]  I. Vesely,et al.  Fabrication of mitral valve chordae by directed collagen gel shrinkage. , 2003, Tissue engineering.

[16]  K. An,et al.  Biochemical, histological, and biomechanical analyses of canine tendon , 1987, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[17]  F. Coustry,et al.  Cultures of fibroblasts in fibrin lattices: Models for the study of metabolic activities of the cells in physiological conditions , 1989, Journal of cellular physiology.

[18]  L. Soslowsky,et al.  Development of tendon structure and function: regulation of collagen fibrillogenesis. , 2005, Journal of musculoskeletal & neuronal interactions.

[19]  Joseph W Freeman,et al.  Collagen self-assembly and the development of tendon mechanical properties. , 2003, Journal of biomechanics.

[20]  J. Aubin,et al.  Association between tension and orientation of periodontal ligament fibroblasts and exogenous collagen fibres in collagen gels in vitro. , 1982, Journal of cell science.

[21]  Mark Eastwood,et al.  Quantitative analysis of collagen gel contractile forces generated by dermal fibroblasts and the relationship to cell morphology , 1996, Journal of cellular physiology.

[22]  Kai-Nan An,et al.  Flexibility of type I collagen and mechanical property of connective tissue. , 2004, Biorheology.

[23]  R T Tranquillo,et al.  An anisotropic biphasic theory of tissue-equivalent mechanics: the interplay among cell traction, fibrillar network deformation, fibril alignment, and cell contact guidance. , 1997, Journal of biomechanical engineering.

[24]  L. Dahners,et al.  Cell populations of tendon: A simplified method for isolation of synovial cells and internal fibroblasts: Confirmation of origin and biologic properties , 1988, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[25]  D L Butler,et al.  Functional tissue engineering: the role of biomechanics. , 2000, Journal of biomechanical engineering.

[26]  F. Grinnell,et al.  Fibroblast-collagen-matrix contraction: growth-factor signalling and mechanical loading. , 2000, Trends in cell biology.

[27]  E Bell,et al.  Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[28]  J. Aubin,et al.  Contraction and organization of collagen gels by cells cultured from periodontal ligament, gingiva and bone suggest functional differences between cell types. , 1981, Journal of cell science.