Systems metabolic engineering of Escherichia coli for the heterologous production of high value molecules-a veteran at new shores.

For more than fifty years, Escherichia coli has represented a remarkable success story in industrial biotechnology. Traditionally known as a producer of l-amino acids, E. coli has also entered the precious market of high-value molecules and is becoming a flexible, efficient production platform for various therapeutics, pre-biotics, nutraceuticals and pigments. This tremendous progress is enabled by systems metabolic engineering concepts that integrate systems biology and synthetic biology into the design and engineering of powerful E. coli cell factories.

[1]  M. de Rosa,et al.  Improved fructosylated chondroitin production by kfoC overexpression in E. coli K4. , 2010, Journal of biotechnology.

[2]  C. Wittmann,et al.  Systems metabolic engineering of Escherichia coli for gram scale production of the antitumor drug deoxyviolacein from glycerol , 2014, Biotechnology and bioengineering.

[3]  C. Wittmann,et al.  Bio-based production of chemicals, materials and fuels -Corynebacterium glutamicum as versatile cell factory. , 2012, Current opinion in biotechnology.

[4]  Johannes Gescher,et al.  Bioengineering and Biotechnology Review Article Metabolic Engineering of Escherichia Coli for Production of Mixed-acid Fermentation End Products , 2022 .

[5]  Long Liu,et al.  Metabolic engineering of Escherichia coli BL21 for biosynthesis of heparosan, a bioengineered heparin precursor. , 2012, Metabolic engineering.

[6]  G. Sprenger,et al.  Synthesis of the Human Milk Oligosaccharide Lacto‐N‐Tetraose in Metabolically Engineered, Plasmid‐Free E. coli , 2014, Chembiochem : a European journal of chemical biology.

[7]  Sang Yup Lee,et al.  Systems Metabolic Engineering of Escherichia coli for Chemicals, Materials, Biofuels, and Pharmaceuticals , 2012 .

[8]  K. Lemuth,et al.  Engineering of a plasmid-free Escherichia coli strain for improved in vivo biosynthesis of astaxanthin , 2011, Microbial cell factories.

[9]  Jin-Ho Seo,et al.  Enhanced production of 2'-fucosyllactose in engineered Escherichia coli BL21star(DE3) by modulation of lactose metabolism and fucosyltransferase. , 2015, Journal of biotechnology.

[10]  M. Jiang,et al.  Engineering the shikimate pathway for biosynthesis of molecules with pharmaceutical activities in E. coli. , 2016, Current opinion in biotechnology.

[11]  A. F. Mendes,et al.  Evaluation of the anti-inflammatory, anti-catabolic and pro-anabolic effects of E-caryophyllene, myrcene and limonene in a cell model of osteoarthritis. , 2015, European journal of pharmacology.

[12]  Jay D. Keasling,et al.  High-Level Production of Amorpha-4,11-Diene, a Precursor of the Antimalarial Agent Artemisinin, in Escherichia coli , 2009, PloS one.

[13]  A. Riggs.  Bacterial Production of Human Insulin , 1981, Diabetes Care.

[14]  C. Peña,et al.  Biotechnological strategies to improve production of microbial poly-(3-hydroxybutyrate): a review of recent research work , 2014, Microbial biotechnology.

[15]  Yun Chen,et al.  Enabling technologies to advance microbial isoprenoid production. , 2015, Advances in biochemical engineering/biotechnology.

[16]  M. Rosa,et al.  Production of chondroitin sulfate and chondroitin , 2010, Applied Microbiology and Biotechnology.

[17]  S. C. Kim,et al.  Metabolic engineering of Escherichia coli for the production of cinnamaldehyde , 2016, Microbial Cell Factories.

[18]  C. Wittmann,et al.  Systems metabolic engineering of Escherichia coli for production of the antitumor drugs violacein and deoxyviolacein. , 2013, Metabolic engineering.

[19]  Huimin Yu,et al.  A high‐throughput screen for hyaluronic acid accumulation in recombinant Escherichia coli transformed by libraries of engineered sigma factors , 2008, Biotechnology and bioengineering.

[20]  J. Revuelta,et al.  Biotechnology of riboflavin , 2016, Applied Microbiology and Biotechnology.

[21]  Uwe Beifuss,et al.  Synthesis of fucosylated lacto-N-tetraose using whole-cell biotransformation. , 2015, Bioorganic & medicinal chemistry.

[22]  G. Stephanopoulos,et al.  Identifying gene targets for the metabolic engineering of lycopene biosynthesis in Escherichia coli. , 2005, Metabolic engineering.

[23]  Xueli Zhang,et al.  Engineering central metabolic modules of Escherichia coli for improving β-carotene production. , 2013, Metabolic engineering.

[24]  G. Loake,et al.  Paclitaxel: biosynthesis, production and future prospects. , 2014, New biotechnology.

[25]  Xixian Chen,et al.  Experimental design-aided systematic pathway optimization of glucose uptake and deoxyxylulose phosphate pathway for improved amorphadiene production , 2015, Applied Microbiology and Biotechnology.

[26]  H. Huang.  Production of L-threonine by auxotrophic mutants of Escherichia coli. , 1961, Applied microbiology.

[27]  Tong Un Chae,et al.  Metabolic engineering of Escherichia coli for the production of 1,3-diaminopropane, a three carbon diamine , 2013, Scientific Reports.

[28]  Jay D Keasling,et al.  Narrowing the gap between the promise and reality of polyketide synthases as a synthetic biology platform. , 2014, Current opinion in biotechnology.

[29]  C. Paddon,et al.  Developing fermentative terpenoid production for commercial usage. , 2016, Current opinion in biotechnology.

[30]  J. Nielsen,et al.  Advancing metabolic engineering through systems biology of industrial microorganisms. , 2015, Current opinion in biotechnology.

[31]  V. Wendisch,et al.  Engineering microbial cell factories: Metabolic engineering of Corynebacterium glutamicum with a focus on non‐natural products , 2015, Biotechnology journal.

[32]  Hal S Alper,et al.  Frontiers of yeast metabolic engineering: diversifying beyond ethanol and Saccharomyces. , 2013, Current opinion in biotechnology.

[33]  Christoph Wittmann,et al.  Diamines for Bio‐Based Materials , 2016 .

[34]  M. Rosa,et al.  IS2-mediated overexpression of kfoC in E. coli K4 increases chondroitin-like capsular polysaccharide production , 2014, Applied Microbiology and Biotechnology.

[35]  G. Sprenger,et al.  Construction of Escherichia coli strains with chromosomally integrated expression cassettes for the synthesis of 2′-fucosyllactose , 2013, Microbial Cell Factories.

[36]  Masato Ikeda,et al.  A novel gnd mutation leading to increased L-lysine production in Corynebacterium glutamicum. , 2005, FEMS microbiology letters.

[37]  J. Revuelta,et al.  Three biotechnical processes using Ashbya gossypii, Candida famata, or Bacillus subtilis compete with chemical riboflavin production , 2000, Applied Microbiology and Biotechnology.

[38]  H. Schroten,et al.  Bioengineered 2'-fucosyllactose and 3-fucosyllactose inhibit the adhesion of Pseudomonas aeruginosa and enteric pathogens to human intestinal and respiratory cell lines. , 2013, Nutrition research.

[39]  Xueli Zhang,et al.  Production of salidroside in metabolically engineered Escherichia coli , 2014, Scientific Reports.

[40]  Gregory Stephanopoulos,et al.  Construction of lycopene-overproducing E. coli strains by combining systematic and combinatorial gene knockout targets , 2005, Nature Biotechnology.

[41]  A. Sharma,et al.  Microbial pigments as natural color sources: current trends and future perspectives , 2015, Journal of Food Science and Technology.

[42]  Keith E. J. Tyo,et al.  Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli , 2010, Science.

[43]  Tao Chen,et al.  Metabolic engineering of Escherichia coli for the production of riboflavin , 2014, Microbial Cell Factories.

[44]  G. Stephanopoulos,et al.  Distributing a metabolic pathway among a microbial consortium enhances production of natural products , 2015, Nature Biotechnology.

[45]  M. Koffas,et al.  Microbial production of value-added nutraceuticals. , 2016, Current opinion in biotechnology.

[46]  Michael J. Miller,et al.  Whole cell biosynthesis of a functional oligosaccharide, 2′-fucosyllactose, using engineered Escherichia coli , 2012, Microbial Cell Factories.

[47]  A. Schreiber,et al.  Designer amphiphilic proteins as building blocks for the intracellular formation of organelle-like compartments. , 2015, Nature materials.

[48]  Masafumi Kaneko,et al.  Heterologous production of flavanones in Escherichia coli: potential for combinatorial biosynthesis of flavonoids in bacteria , 2003, Journal of Industrial Microbiology and Biotechnology.

[49]  Christoph Wittmann,et al.  Advanced biotechnology: metabolically engineered cells for the bio-based production of chemicals and fuels, materials, and health-care products. , 2015, Angewandte Chemie.

[50]  Tiangang Liu,et al.  In vitro reconstitution of mevalonate pathway and targeted engineering of farnesene overproduction in Escherichia coli , 2014, Biotechnology and bioengineering.

[51]  J. Mikkelsen,et al.  Novel α-L-Fucosidases from a Soil Metagenome for Production of Fucosylated Human Milk Oligosaccharides , 2016, PloS one.

[52]  Christoph Wittmann,et al.  Metabolic flux engineering of L-lysine production in Corynebacterium glutamicum--over expression and modification of G6P dehydrogenase. , 2007, Journal of biotechnology.

[53]  Christoph Wittmann,et al.  Systems and synthetic metabolic engineering for amino acid production - the heartbeat of industrial strain development. , 2012, Current opinion in biotechnology.

[54]  Yi Tang,et al.  Natural and engineered production of taxadiene with taxadiene synthase , 2015, Biotechnology and bioengineering.

[55]  M. de Rosa,et al.  Isolation of an Escherichia coli K4 kfoC mutant over-producing capsular chondroitin , 2010, Microbial cell factories.

[56]  N. Braidy,et al.  Rhodiola rosea L. and Alzheimer's Disease: From Farm to Pharmacy , 2016, Phytotherapy research : PTR.

[57]  R. Lewis,et al.  Structure of a protein superfiber: spider dragline silk. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[58]  Fritz Vollrath,et al.  Characterization of the protein components of Nephila clavipes dragline silk. , 2005, Biochemistry.

[59]  R. Linhardt,et al.  High cell density cultivation of a recombinant E. coli strain expressing a key enzyme in bioengineered heparin production , 2013, Applied Microbiology and Biotechnology.

[60]  Bin Jia,et al.  Engineered biosynthesis of natural products in heterologous hosts. , 2015, Chemical Society reviews.

[61]  Pablo Carbonell,et al.  Synthetic biology for pharmaceutical drug discovery , 2015, Drug design, development and therapy.

[62]  Seon-Won Kim,et al.  Combinatorial engineering of hybrid mevalonate pathways in Escherichiacoli for protoilludene production , 2016, Microbial Cell Factories.

[63]  R. Croteau,et al.  Engineering Escherichia coli for the synthesis of taxadiene, a key intermediate in the biosynthesis of taxol. , 2001, Bioorganic & medicinal chemistry.

[64]  J. Keasling,et al.  Engineering a mevalonate pathway in Escherichia coli for production of terpenoids , 2003, Nature Biotechnology.

[65]  H. Woo,et al.  Microbial Synthesis of Myrcene by Metabolically Engineered Escherichia coli. , 2015, Journal of agricultural and food chemistry.

[66]  G. Stephanopoulos,et al.  Metabolic engineering of Escherichia coli for biosynthesis of hyaluronic acid. , 2008, Metabolic engineering.

[67]  E. Park,et al.  Riboflavin production by Ashbya gossypii , 2011, Biotechnology Letters.

[68]  Johannes Gescher,et al.  The molecular toolbox for chromosomal heterologous multiprotein expression in Escherichia coli. , 2012, Biochemical Society transactions.

[69]  C. Wittmann,et al.  Top value platform chemicals: bio-based production of organic acids. , 2015, Current opinion in biotechnology.

[70]  G. Stephanopoulos,et al.  Compartmentalization of metabolic pathways in yeast mitochondria improves production of branched chain alcohols , 2013, Nature Biotechnology.

[71]  J. Keasling,et al.  High-level semi-synthetic production of the potent antimalarial artemisinin , 2013, Nature.

[72]  Young Hwan Park,et al.  Native-sized recombinant spider silk protein produced in metabolically engineered Escherichia coli results in a strong fiber , 2010, Proceedings of the National Academy of Sciences.

[73]  R. Linhardt,et al.  Production of chondroitin in metabolically engineered E. coli. , 2015, Metabolic engineering.

[74]  X. Xing,et al.  High crude violacein production from glucose by Escherichia coli engineered with interactive control of tryptophan pathway and violacein biosynthetic pathway , 2015, Microbial Cell Factories.

[75]  Robert M. Long,et al.  Genetic engineering of taxol biosynthetic genes in Saccharomyces cerevisiae. , 2006, Biotechnology and bioengineering.

[76]  Christoph Wittmann,et al.  Microbial production of the drugs violacein and deoxyviolacein: analytical development and strain comparison , 2011, Biotechnology Letters.

[77]  Jian-Zhong Liu,et al.  Metabolic engineering of Escherichia coli to produce zeaxanthin , 2015, Journal of Industrial Microbiology & Biotechnology.

[78]  R. Linhardt,et al.  ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways , 2015, Scientific Reports.

[79]  J. Keasling,et al.  Semi-synthetic artemisinin: a model for the use of synthetic biology in pharmaceutical development , 2014, Nature Reviews Microbiology.

[80]  Guillermo Gosset,et al.  Production of aromatic compounds in bacteria. , 2009, Current opinion in biotechnology.

[81]  Pyung-Cheon Lee,et al.  Metabolic engineering of the Stevia rebaudiana ent-kaurene biosynthetic pathway in recombinant Escherichia coli. , 2015, Journal of biotechnology.

[82]  Joong-Hoon Ahn,et al.  Biological synthesis of coumarins in Escherichia coli , 2015, Microbial Cell Factories.

[83]  B. Priem,et al.  Production of intracellular heparosan and derived oligosaccharides by lyase expression in metabolically engineered E. coli K-12. , 2012, Carbohydrate research.