Raman study of the effects of polyamines on DNA:spermine and histamine

Abstract Fourier transform Raman spectroscopy was used to investigate the interaction of spermine and histamine with calf-thymus DNA. Polyamine–DNA solutions at different polyamine concentrations ranging from 5 mM to 75 mM were prepared. For spermine, solutions no higher than 15 mM were prepared because this molecule induces condensation and aggregation on DNA at upper concentrations. Possible sites of bindings for polyamine–DNA complexes were discussed on the basis of the spectral changes observed with respect to the Raman spectra of DNA. The results seem to indicate that one spermine molecule induces on DNA a similar effect to two or more histamine molecules.

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