Genome-wide Targeted Mutagenesis in Rice Using the CRISPR/Cas9 System.

Since the completion of the rice (Oryza sativa) genome-sequencing project, a major goal of rice research has been the functional characterization of all annotated genetic loci in various biological processes. One of the most efficient and widely-used strategies for studying gene function is genetic mutagenesis. Several rice mutant libraries have been generated in the past decade, providing a wealth of resources for plant research (Chang et al., 2012). CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats–associated nuclease 9) has recently emerged as a powerful tool for rice research and breeding (Cong et al., 2013; Feng et al., 2013; Miao et al., 2013; Shan et al., 2013; Sun et al., 2016).

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