Evaluation of the Bacteriostatic and Bactericidal Activity of Essential Oil of Thymus Satureioides

Essential oils have significant antimicrobial activities and can successfully replace antibiotic s that show their inefficiencies against resistant microorganisms [1]. The objective of this study is to evaluate the antibacterial activity of essential oils extracted from the leaves and flowering tops of Thymus satureioides against three gram-negative bacteria (Escherichia coli, Acinetobacter baumannii and Enterobacter cloacae) and two gram-positive bacteria (Bacillus cereus and Staphylococcus aureus). The extraction is carried out by hydrodistillation using a Clevenger-type apparatus with an average yield of 3.4%. The antibacterial activity is highlighted by the diffusion method on agar medium. A Strong inhibitory activity against bacteria isolated was recorded. However, the bactericidal activity is remarkable against all strains tested except Bacillus cereus.

[1]  D. Leach,et al.  Antioxidative activity and synergistic effect of Thymus saturejoides Coss. essential oils with cefixime against selected food-borne bacteria , 2014 .

[2]  D. Leach,et al.  Chemical composition, antioxidant and antimicrobial activities of essential oils obtained from wild and cultivated Moroccan Thymus species , 2013 .

[3]  T. Simmet,et al.  Hypolipidemic and antioxidant effect of polyphenol-rich extracts from Moroccan thyme varieties , 2012 .

[4]  B. Satrani,et al.  Effet de l’Age et du Stade Vegetatif sur la Teneur et la Composition Chimique des Huiles Essentielles de Thuya de Berbere , 2011 .

[5]  K. Elhabazi,et al.  Analgesic activity of three thyme species, Thymus satureioides, Thymus maroccanus and Thymus leptobotrys , 2008 .

[6]  L. Mayaud,et al.  Comparison of bacteriostatic and bactericidal activity of 13 essential oils against strains with varying sensitivity to antibiotics , 2008, Letters in applied microbiology.

[7]  M. Lahlou Methods to study the phytochemistry and bioactivity of essential oils , 2004, Phytotherapy research : PTR.

[8]  L. Milella,et al.  In vivo topical anti-inflammatory and in vitro antioxidant activities of two extracts of Thymus satureioides leaves. , 2004, Journal of ethnopharmacology.

[9]  B. Timmermann,et al.  Antibacterial diterpenes from Calceolaria pinifolia. , 2003, Journal of natural products.

[10]  Ö. Erdoğrul Antibacterial Activities of Some Plant Extracts Used in Folk Medicine , 2002 .

[11]  A. Chaouch,et al.  Influence de la durée et de la technique d'extraction sur le rendement et la qualite des huiles essentielles du cèdre de l'Atlas (Cedrus atlantica Manetti) , 2001 .

[12]  G. Hageman,et al.  Antibacterial effect of garlic and omeprazole on Helicobacter pylori. , 1999, The Journal of antimicrobial chemotherapy.

[13]  T. Riley,et al.  Broth micro-dilution method for determining the susceptibility of Escherichia coli and Staphylococcus aureus to the essential oil of Melaleuca alternifolia (tea tree oil). , 1995, Microbios.

[14]  B. Benjilali,et al.  Composition and Antimicrobial Activity of the Essential Oils of Thymus broussonettii, T. zygis and T. satureioides , 1993 .