Membrane-based inverse-transition purification facilitates a rapid isolation of various spider-silk elastin-like polypeptide fusion proteins from extracts of transgenic tobacco

[1]  J. Buyel,et al.  Product safety aspects of plant molecular farming , 2023, Frontiers in bioengineering and biotechnology.

[2]  J. Buyel,et al.  Killer to cure: Expression and production costs calculation of tobacco plant‐made cancer‐immune checkpoint inhibitors , 2023, Plant biotechnology journal.

[3]  F. Mottaghy,et al.  Simple plant‐based production and purification of the assembled human ferritin heavy chain as a nanocarrier for tumor‐targeted drug delivery and bioimaging in cancer therapy , 2022, Biotechnology and bioengineering.

[4]  C. Oostenbrink,et al.  CASPON platform technology: Ultrafast circularly permuted caspase-2 cleaves tagged fusion proteins before all 20 natural amino acids at the N-terminus. , 2022, New biotechnology.

[5]  J. Buyel,et al.  Nicotiana spp. for the Expression and Purification of Functional IgG3 Antibodies Directed Against the Staphylococcus aureus Alpha Toxin , 2021, Frontiers in Chemical Engineering.

[6]  F. Salaün,et al.  Silkworm and spider silk electrospinning: a review , 2021, Environmental Chemistry Letters.

[7]  J. Buyel,et al.  Seasonal Weather Changes Affect the Yield and Quality of Recombinant Proteins Produced in Transgenic Tobacco Plants in a Greenhouse Setting , 2019, Front. Plant Sci..

[8]  S. Schillberg,et al.  Critical Analysis of the Commercial Potential of Plants for the Production of Recombinant Proteins , 2019, Front. Plant Sci..

[9]  C. Marusic,et al.  Current state-of-the-art in plant-based antibody production systems , 2019, Biotechnology Letters.

[10]  J. Buyel,et al.  A Combined Ultrafiltration/Diafiltration Step Facilitates the Purification of Cyanovirin-N From Transgenic Tobacco Extracts , 2019, Front. Bioeng. Biotechnol..

[11]  J. Buyel,et al.  Expression and purification of human phosphatase and actin regulator 1 (PHACTR1) in plant-based systems. , 2018, Protein expression and purification.

[12]  J. Buyel,et al.  Downstream processing of a plant-derived malaria transmission-blocking vaccine candidate. , 2018, Protein expression and purification.

[13]  U. Conrad,et al.  Low-Tech, Pilot Scale Purification of a Recombinant Spider Silk Protein Analog from Tobacco Leaves , 2016, International journal of molecular sciences.

[14]  K. Moustafa,et al.  Molecular farming on rescue of pharma industry for next generations , 2016, Critical reviews in biotechnology.

[15]  E. Hood,et al.  Plant Molecular Farming: Much More than Medicines. , 2016, Annual review of analytical chemistry.

[16]  J. Buyel,et al.  Depth Filters Containing Diatomite Achieve More Efficient Particle Retention than Filters Solely Containing Cellulose Fibers , 2015, Front. Plant Sci..

[17]  J. Buyel Process Development Strategies in Plant Molecular Farming. , 2015, Current pharmaceutical biotechnology.

[18]  K. Reimers,et al.  In planta production of ELPylated spidroin-based proteins results in non-cytotoxic biopolymers , 2015, BMC Biotechnology.

[19]  J. Buyel,et al.  Rational design of a host cell protein heat precipitation step simplifies the subsequent purification of recombinant proteins from tobacco , 2014 .

[20]  Friedrich Altmann,et al.  Influence of Elastin-Like Polypeptide and Hydrophobin on Recombinant Hemagglutinin Accumulations in Transgenic Tobacco Plants , 2014, PloS one.

[21]  J. Buyel,et al.  Scale-down models to optimize a filter train for the downstream purification of recombinant pharmaceutical proteins produced in tobacco leaves. , 2014, Biotechnology journal.

[22]  L. Domingues,et al.  Fusion tags for protein solubility, purification and immunogenicity in Escherichia coli: the novel Fh8 system , 2014, Front. Microbiol..

[23]  Rainer Fischer,et al.  Flocculation increases the efficacy of depth filtration during the downstream processing of recombinant pharmaceutical proteins produced in tobacco. , 2014, Plant biotechnology journal.

[24]  U. Conrad,et al.  Transglutamination allows production and characterization of native-sized ELPylated spider silk proteins from transgenic plants. , 2014, Plant biotechnology journal.

[25]  Ana Sofia Pina,et al.  Challenges and opportunities in the purification of recombinant tagged proteins , 2013, Biotechnology Advances.

[26]  Gerd Hause,et al.  ELPylated haemagglutinins produced in tobacco plants induce potentially neutralizing antibodies against H5N1 viruses in mice. , 2013, Plant biotechnology journal.

[27]  A. Chilkoti,et al.  Predicting transition temperatures of elastin-like polypeptide fusion proteins. , 2013, Biomacromolecules.

[28]  A. Robinson,et al.  Recombinant protein expression and purification: A comprehensive review of affinity tags and microbial applications , 2012, Biotechnology journal.

[29]  L. Wilken,et al.  Recovery and purification of plant-made recombinant proteins. , 2012, Biotechnology advances.

[30]  Samuel S. M. Sun,et al.  A Cost-Effective ELP-Intein Coupling System for Recombinant Protein Purification from Plant Production Platform , 2011, PloS one.

[31]  U. Conrad,et al.  Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant Protein-Elastin-Like Polypeptide Fusions , 2011, International journal of molecular sciences.

[32]  A. Xu,et al.  An improved nonchromatographic method for the purification of recombinant proteins using elastin-like polypeptide-tagged proteases , 2011, Analytical Biochemistry.

[33]  Yifeng Li Self-cleaving fusion tags for recombinant protein production , 2011, Biotechnology Letters.

[34]  A. Chilkoti,et al.  Elastin‐Like Polypeptides as a Purification Tag for Recombinant Proteins , 2010, Current protocols in protein science.

[35]  Thomas W Patapoff,et al.  Cold denaturation of monoclonal antibodies , 2010, mAbs.

[36]  J. Scheller,et al.  Influence of elastin-like peptide fusions on the quantity and quality of a tobacco-derived human immunodeficiency virus-neutralizing antibody. , 2009, Plant biotechnology journal.

[37]  Rima Menassa,et al.  Optimization of elastin‐like polypeptide fusions for expression and purification of recombinant proteins in plants , 2009, Biotechnology and bioengineering.

[38]  P. Scheerer,et al.  The structure of the anti‐c‐myc antibody 9E10 Fab fragment/epitope peptide complex reveals a novel binding mode dominated by the heavy chain hypervariable loops , 2008, Proteins.

[39]  Karl Fischer,et al.  Temperature triggered self-assembly of polypeptides into multivalent spherical micelles. , 2008, Journal of the American Chemical Society.

[40]  A. Kim Permeate flux inflection due to concentration polarization in crossflow membrane filtration: A novel analytic approach , 2007, The European physical journal. E, Soft matter.

[41]  A. Sytkowski,et al.  Variability in the immunodetection of His-tagged recombinant proteins. , 2006, Analytical biochemistry.

[42]  J. Bischof,et al.  Thermal Stability of Proteins , 2005, Annals of the New York Academy of Sciences.

[43]  M. Miao,et al.  Sequence and Structure Determinants for the Self-aggregation of Recombinant Polypeptides Modeled after Human Elastin* , 2003, Journal of Biological Chemistry.

[44]  K. Hilpert,et al.  Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose. , 2001, Protein engineering.

[45]  J. Scheller,et al.  Production of spider silk proteins in tobacco and potato , 2001, Nature Biotechnology.

[46]  Ashutosh Chilkoti,et al.  Purification of recombinant proteins by fusion with thermally-responsive polypeptides , 1999, Nature Biotechnology.

[47]  T. M. Parker,et al.  Hydrophobicity scale for proteins based on inverse temperature transitions , 1992, Biopolymers.

[48]  Dan W. Urry,et al.  Entropic elastic processes in protein mechanisms. I. Elastic structure due to an inverse temperature transition and elasticity due to internal chain dynamics , 1988, Journal of protein chemistry.

[49]  R. Gosu,et al.  Introduction to Surface Plasmon Resonance , 2021, Methods for Fragments Screening Using Surface Plasmon Resonance.

[50]  A. Chilkoti,et al.  Fusions of elastin-like polypeptides to pharmaceutical proteins. , 2012, Methods in enzymology.

[51]  J. Scheller,et al.  Elastin-like polypeptides revolutionize recombinant protein expression and their biomedical application. , 2010, Trends in biotechnology.

[52]  Abhinav A Shukla,et al.  Exploitation of the Adsorptive Properties of Depth Filters for Host Cell Protein Removal during Monoclonal Antibody Purification , 2006, Biotechnology progress.