Effect of biphasic calcium phosphate on human macrophage functions in vitro.

Bioceramics may initiate several and complex biological reactions in host tissues. The cell-biomaterial interaction can determine macrophage activation that may elicit and sustain inflammatory response at the implant site. The current study describes some of the in vitro phenomena regarding the effect of surface reactivity of biphasic calcium phosphate (BCP) granules on human macrophages locomotion and secretion. X-ray diffraction analysis indicated that the synthesized ceramic presented 80% hydroxyapatite and 20% tricalcium phosphate. When BCP was put in contact with human macrophage cells, we observed that cells and BCP granules attached to each other. Cells attached to BCP presented a higher intracellular free Ca(2+) concentration compared with nonattached neighbors and secreted calcium phosphate particles into the medium. Energy dispersive X-ray analysis showed that the secreted particles presented a calcium/phosphorus ratio of 1.64 +/- 0.05, similar to hydroxyapatite. We propose that the secreted particles create a transition zone that allows further macrophage adhesion.

[1]  C. Naus,et al.  Intercellular Calcium Signaling in Astrocytes via ATP Release through Connexin Hemichannels* , 2002, The Journal of Biological Chemistry.

[2]  John A Jansen,et al.  Trabecular bone response to surface roughened and calcium phosphate (Ca-P) coated titanium implants. , 2002, Biomaterials.

[3]  A. Ito,et al.  Fabrication of Zn containing apatite cement and its initial evaluation using human osteoblastic cells. , 2002, Biomaterials.

[4]  M. Romanello,et al.  Mechanically induced ATP release from human osteoblastic cells. , 2001, Biochemical and biophysical research communications.

[5]  D. Nelson,et al.  Calcium-G Protein Interactions in the Regulation of Macrophage Secretion* , 2001, The Journal of Biological Chemistry.

[6]  Amal K. Dutta,et al.  Volume-Dependent Atp-Conductive Large-Conductance Anion Channel as a Pathway for Swelling-Induced Atp Release , 2001, The Journal of general physiology.

[7]  A. Davalli,et al.  Fluorescent dyes for cell viability: an application on prefixed conditions , 2001, Histochemistry and Cell Biology.

[8]  I. Macintyre,et al.  The biphasic effects of nitric oxide in primary rat osteoblasts are cGMP dependent. , 2000, Biochemical and biophysical research communications.

[9]  P Ducheyne,et al.  Bioactive ceramics: the effect of surface reactivity on bone formation and bone cell function. , 1999, Biomaterials.

[10]  G. Daculsi,et al.  Short-term effects of mineral particle sizes on cellular degradation activity after implantation of injectable calcium phosphate biomaterials and the consequences for bone substitution. , 1999, Bone.

[11]  P. Ducheyne,et al.  Effect of serum proteins on osteoblast adhesion to surface‐modified bioactive glass and hydroxyapatite , 1999, Journal of Orthopaedic Research.

[12]  D. Zukor,et al.  Induction of macrophage apoptosis by ceramic and polyethylene particles in vitro. , 1999, Biomaterials.

[13]  M. Raspanti,et al.  Structural and functional macrophages alterations by ceramics of different composition. , 1999, Biomaterials.

[14]  J. Planell,et al.  Growth and differentiation of human bone marrow osteoprogenitors on novel calcium phosphate cements. , 1998, Biomaterials.

[15]  T. Ohsumi,et al.  Evaluation of cytotoxicity of calcium phosphate cement consisting of alpha-tricalcium phosphate and dicalcium phosphate dihydrate. , 1998, Dental materials journal.

[16]  G. Daculsi,et al.  Biphasic calcium phosphate concept applied to artificial bone, implant coating and injectable bone substitute. , 1998, Biomaterials.

[17]  D. N. Silva-Teixeira,et al.  Role of Nitric Oxide on Human Schistosomiasis Mansoni: Upregulation ofin VitroGranuloma Formation byNω-Nitro-l-arginine Methyl Ester☆ , 1998 .

[18]  I. Kennedy,et al.  ATP is released from rabbit urinary bladder epithelial cells by hydrostatic pressure changes–possible sensory mechanism? , 1997, The Journal of physiology.

[19]  G. Daculsi,et al.  Osteoclastic resorption of calcium phosphate ceramics with different hydroxyapatite/beta-tricalcium phosphate ratios. , 1997, Biomaterials.

[20]  T. Chin,et al.  Hydroxyapatite synthesized by a simplified hydrothermal method , 1997 .

[21]  P. Ducheyne,et al.  Formation of surface reaction products on bioactive glass and their effects on the expression of the osteoblastic phenotype and the deposition of mineralized extracellular matrix. , 1997, Biomaterials.

[22]  S. Ralston,et al.  Nitric oxide and bone , 2001, Immunology.

[23]  G. Gronowicz,et al.  Response of human osteoblasts to implant materials: Integrin‐mediated adhesion , 1996, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[24]  O. Gan,et al.  Biodegradation of synthetic biphasic calcium phosphate by human monocytes in vitro: a morphological study. , 1996, Biomaterials.

[25]  M. Nathanson,et al.  Isolated rat hepatocytes can signal to other hepatocytes and bile duct cells by release of nucleotides. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[26]  B. Nebe,et al.  Mechanical induction of beta 1-integrin-mediated calcium signaling in a hepatocyte cell line. , 1995, Experimental cell research.

[27]  P. Hauschka,et al.  Cytokines induce nitric oxide production in mouse osteoblasts. , 1994, Biochemical and biophysical research communications.

[28]  J. Ricci,et al.  Evaluation of a low-temperature calcium phosphate particulate implant material: physical-chemical properties and in vivo bone response. , 1992, Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons.

[29]  S. Pollack,et al.  The effect of phase differences on the time-dependent variation of the zeta potential of hydroxyapatite. , 1992, Journal of biomedical materials research.

[30]  T Kitsugi,et al.  Solutions able to reproduce in vivo surface-structure changes in bioactive glass-ceramic A-W. , 1990, Journal of biomedical materials research.

[31]  T. Yamamuro,et al.  The influence of calcium phosphate ceramics and glass-ceramics on cultured cells and their surrounding media. , 1989, Journal of biomedical materials research.