Wound healing activity of methanolic extract of Achyranthes aspera Linn. was evaluated by excision and incision wound model (in vivo) in Sprague Dawley rats and by chorioallantoic membrane (CAM) model (in vitro) in 9-day-old embryonated chicken eggs. In excision wound, there was 82.23% wound contraction in control group on 21st day, whereas in A. aspera treated group, complete healing took place on 21st day. In the standard group, the wound persisted beyond 21st day. These findings were confirmed by histopathological examination. In incision wound, tensile strength of the healing tissue of A. aspera treated group was comparable with standard group and both were significantly higher compared to the control group. In CAM model, angiogenesis in A. aspera treated group @ 400 μg/disc was compared to the control group suggesting promising wound healing activity of methanolic extract of A. aspera.