Salivary hexosaminidase B in children with type 1 diabetes

__________________________________________________________________________________________ Introduction: Children type 1 diabetes is accompanied by inflammation and microangiopathy pro-ceeded with increased degradation of tissues. Nacetyl-β-hexosaminidase (HEX) is the most active of exoglycosidases degrading oligo-saccharide chains of glycoconjugates (glycoproteins, glycolipids and proteoglycans). Purpose: To evaluate the hexosaminidase B (HEX B) activity in the saliva of children with type 1 diabetes. Materials and methods: The study was performed in 35 children with type 1 diabetes and 20 healthy children. Salivary HEX B activity was determined by the colorimetric, and protein by bicinchoninic acid methods. The HEX B activity concentration was expressed in pKat/mL and specific activity in

[1]  C. Taplin,et al.  Insulin Therapy in Children and Adolescents with Type 1 Diabetes , 2014, Pediatric Drugs.

[2]  Tom,et al.  Zalecenia kliniczne dotyczące postępowania u chorych na cukrzycę, 2013 , 2013 .

[3]  A. Kowalczyk,et al.  [Saliva as a main component of oral cavity ecosystem. Part I. Secretion and function]. , 2007, Wiadomosci lekarskie.

[4]  K. Zwierz,et al.  [Saliva as a main component of oral cavity ecosystem. Part II. Defense mechanisms]. , 2007, Wiadomosci lekarskie.

[5]  K. Zwierz,et al.  Optimization of an enzymatic method for the determination of lysosomal N-acetyl-beta-D-hexosaminidase and beta-glucuronidase in synovial fluid. , 2006, Clinical chemistry and laboratory medicine.

[6]  B. Winchester,et al.  REVIEW Lysosomal metabolism of glycoproteins , 2005 .

[7]  K. Malik,et al.  beta-hexosaminidase-induced activation of p44/42 mitogen-activated protein kinase is dependent on p21Ras and protein kinase C and mediates bovine airway smooth-muscle proliferation. , 1999, American journal of respiratory cell and molecular biology.

[8]  K. Zwierz,et al.  Isoenzymes of N-acetyl-beta-hexosaminidase. , 1999, Acta biochimica Polonica.

[9]  C. Christensen,et al.  Clinical Criteria for the Diagnosis of Salivary Gland Hypofunction , 1992, Journal of dental research.

[10]  M. Kawamura,et al.  Changes in rat submandibular gland N-acetyl-beta-glucosaminidase activity in streptozotocin-induced diabetes. , 1989, Journal of Osaka Dental University.

[11]  C. Dawes.  Physiological Factors Affecting Salivary Flow Rate, Oral Sugar Clearance, and the Sensation of Dry Mouth in Man , 1987 .

[12]  K. Zwierz,et al.  The degradation of glycoconjugates in the human gastric mucous membrane. , 1981, Acta medica Academiae Scientiarum Hungaricae.