Influence of the Hydrodynamic Environment on Quorum Sensing in Pseudomonas aeruginosa Biofilms

ABSTRACT We provide experimental and modeling evidence that the hydrodynamic environment can impact quorum sensing (QS) in a Pseudomonas aeruginosa biofilm. The amount of biofilm biomass required for full QS induction of the population increased as the flow rate increased.

[1]  J. Costerton,et al.  Gene Expression in Pseudomonas aeruginosa: Evidence of Iron Override Effects on Quorum Sensing and Biofilm-Specific Gene Regulation , 2001, Journal of bacteriology.

[2]  E. Greenberg,et al.  Identification, Timing, and Signal Specificity of Pseudomonas aeruginosa Quorum-Controlled Genes: a Transcriptome Analysis , 2003, Journal of bacteriology.

[3]  J. Heijnen,et al.  Influence of different substrates on the formation of biofilms in a biofilm airlift suspension reactor , 2000 .

[4]  M. Surette,et al.  Environmental Regulation of Pseudomonas aeruginosa PAO1 Las and Rhl Quorum-Sensing Systems , 2007, Journal of bacteriology.

[5]  E. Greenberg,et al.  Analysis of random and site‐directed mutations in rhlI, a Pseudomonas aeruginosa gene encoding an acylhomoserine lactone synthase , 1997, Molecular microbiology.

[6]  Characklis.  Biofilm development and destruction , 1980 .

[7]  H. Lappin-Scott,et al.  The formation of migratory ripples in a mixed species bacterial biofilm growing in turbulent flow. , 1999, Environmental microbiology.

[8]  J. Costerton,et al.  Influence of Hydrodynamics and Cell Signaling on the Structure and Behavior of Pseudomonas aeruginosa Biofilms , 2002, Applied and Environmental Microbiology.

[9]  Matthew R. Parsek,et al.  Characterization of Colony Morphology Variants Isolated from Pseudomonas aeruginosa Biofilms , 2005, Applied and Environmental Microbiology.

[10]  E. Greenberg,et al.  Delays in Pseudomonas aeruginosa quorum-controlled gene expression are conditional. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[11]  M. Gambello,et al.  Cloning and characterization of the Pseudomonas aeruginosa lasR gene, a transcriptional activator of elastase expression , 1991, Journal of bacteriology.

[12]  S. Wuertz,et al.  Effect of flow regime on the architecture of a Pseudomonas fluorescens biofilm. , 2002, Biotechnology and bioengineering.

[13]  J. Costerton,et al.  Bacterial biofilms: a common cause of persistent infections. , 1999, Science.

[14]  D. Chopp,et al.  A mathematical model of quorum sensing in a growing bacterial biofilm , 2002, Journal of Industrial Microbiology and Biotechnology.

[15]  W. Fuqua,et al.  Evidence of autoinducer activity in naturally occurring biofilms. , 1997, FEMS microbiology letters.

[16]  E. Greenberg,et al.  Signalling: Listening in on bacteria: acyl-homoserine lactone signalling , 2002, Nature Reviews Molecular Cell Biology.

[17]  E. Greenberg,et al.  Regulation of gene expression by cell-to-cell communication: acyl-homoserine lactone quorum sensing. , 2001, Annual review of genetics.

[18]  B. Ersbøll,et al.  Quantification of biofilm structures by the novel computer program COMSTAT. , 2000, Microbiology.

[19]  Matthew R. Parsek,et al.  Quorum-sensing signals indicate that cystic fibrosis lungs are infected with bacterial biofilms , 2000, Nature.

[20]  E. Greenberg,et al.  Structure of the autoinducer required for expression of Pseudomonas aeruginosa virulence genes. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[21]  D. Chopp,et al.  The dependence of quorum sensing on the depth of a growing biofilm , 2003, Bulletin of mathematical biology.

[22]  E. Greenberg,et al.  Acylated homoserine lactone detection in Pseudomonas aeruginosa biofilms by radiolabel assay. , 2001, Methods in enzymology.

[23]  K. M. Lee,et al.  Identification of genes controlled by quorum sensing in Pseudomonas aeruginosa. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[24]  B. Bassler,et al.  Quorum sensing: cell-to-cell communication in bacteria. , 2005, Annual review of cell and developmental biology.

[25]  Paul Stoodley,et al.  The effect of the chemical, biological, and physical environment on quorum sensing in structured microbial communities , 2006, Analytical and bioanalytical chemistry.

[26]  E. Greenberg,et al.  Interchangeability and specificity of components from the quorum-sensing regulatory systems of Vibrio fischeri and Pseudomonas aeruginosa , 1994, Journal of bacteriology.

[27]  M. Parsek,et al.  Bacterial biofilms: an emerging link to disease pathogenesis. , 2003, Annual review of microbiology.

[28]  Cory J. Rupp,et al.  Biofilm material properties as related to shear-induced deformation and detachment phenomena , 2002, Journal of Industrial Microbiology and Biotechnology.