Biotransformation of gallate esters by a pH-stable tannase of mangrove-derived yeast Debaryomyces hansenii

Introduction: Tannase is a crucial enzyme that finds wide applications in the pharmaceutical industry, feed processing, and beverage manufacturing. Although extensive studies have been conducted on tannases from fungi and bacteria, reports on tannases exhibiting favorable pH stability are relatively limited. Methods: In this study, a tannin-degrading strain Debaryomyces hansenii was screened to induce tannase production, and the corresponding tannase coding gene TANF was successfully cloned and expressed in Yarrowia lipolytica. SDS-PAGE analysis revealed that the purified TanF tannase had a molecular weight of approximately 70 kDa. Results and Discussion: The enzyme demonstrated optimal activity at 40°C and retained over 80% of its activity in the range of 35°C–60°C. Of particular interest, TanF exhibited remarkable enzyme activity at pH 5.0 and retained more than 70% of its relative activity across a wide pH range of 3.0–8.0. Furthermore, TanF exhibited broad substrate specificity for gallate esters. The final gallic acid production by TanF from tannic acid achieved 18.32 g/L. Therefore, the excellent properties TanF has been demonstrated to be an efficient tool for the preparation of gallic acid.

[1]  Heba Sayed Mostafa.  Production of low-tannin Hibiscus sabdariffa tea through D-optimal design optimization of the preparation conditions and the catalytic action of new tannase , 2023, Food chemistry: X.

[2]  D. Ibrahim,et al.  The Tannase from red yeast Rhodotorula glutinis: purification and characterization , 2022, Biocatalysis and Biotransformation.

[3]  Fang Wang,et al.  Optimization of a tannase-assisted process for obtaining teas rich in theaflavins from Camelia sinensis leaves , 2022, Food chemistry: X.

[4]  Zhi-Peng Wang,et al.  Laminaria japonica hydrolysate promotes fucoxanthin accumulation in Phaeodactylum tricornutum. , 2021, Bioresource technology.

[5]  Jie Pan,et al.  Characterization of a Robust and pH-Stable Tannase from Mangrove-Derived Yeast Rhodosporidium diobovatum Q95 , 2020, Marine drugs.

[6]  D. Haltrich,et al.  Co-production of gallic acid and a novel cell-associated tannase by a pigment-producing yeast, Sporidiobolus ruineniae A45.2 , 2020, Microbial Cell Factories.

[7]  M. Cao,et al.  Cloning, Secretory Expression and Characterization of a Unique pH-Stable and Cold-Adapted Alginate Lyase , 2020, Marine drugs.

[8]  Y. H. Kim,et al.  Application of high-pressure treatment improves the in vitro protein digestibility of gel-based meat product. , 2020, Food chemistry.

[9]  A. Xiao,et al.  Thermostable Tannase from Aspergillus Niger and Its Application in the Enzymatic Extraction of Green Tea , 2020, Molecules.

[10]  Xingrong Ju,et al.  Effect of Static-State Fermentation on Volatile Composition in Rapeseed Meal. , 2020, Journal of the science of food and agriculture.

[11]  Xinjun Yu,et al.  Integrated Approaches to Reveal Genes Crucial for Tannin Degradation in Aureobasidium melanogenum T9 , 2019, Biomolecules.

[12]  Peng Zhang,et al.  High and efficient isomaltulose production using an engineered Yarrowia lipolytica strain. , 2018, Bioresource technology.

[13]  M. Rashad,et al.  Purification and characterization of a novel tannase produced by Kluyveromyces marxianus using olive pomace as solid support, and its promising role in gallic acid production. , 2018, International journal of biological macromolecules.

[14]  S. Nakagawa,et al.  Discovery of a Novel Alginate Lyase from Nitratiruptor sp. SB155-2 Thriving at Deep-sea Hydrothermal Vents and Identification of the Residues Responsible for Its Heat Stability* , 2016, The Journal of Biological Chemistry.

[15]  C. Nyachoti,et al.  Recent advances in canola meal utilization in swine nutrition , 2016, Journal of animal science and technology.

[16]  C. Madzak.  Yarrowia lipolytica: recent achievements in heterologous protein expression and pathway engineering , 2015, Applied Microbiology and Biotechnology.

[17]  A. Xiao,et al.  Biotransformation of tea catechins using Aspergillus niger tannase prepared by solid state fermentation on tea byproduct , 2015 .

[18]  B. Pati,et al.  Biosynthesis, structural architecture and biotechnological potential of bacterial tannase: a molecular advancement. , 2014, Bioresource technology.

[19]  H. Gruppen,et al.  Characterisation of cell wall polysaccharides from rapeseed (Brassica napus) meal. , 2013, Carbohydrate polymers.

[20]  Hoyong Kim,et al.  Effects of combination processes on the extraction of pectins from rapeseed cake (Brassica napus L.). , 2013, Food chemistry.

[21]  J. Sigoillot,et al.  Rapeseed and sunflower meal: a review on biotechnology status and challenges , 2012, Applied Microbiology and Biotechnology.

[22]  J. A. Jorge,et al.  Extracellular tannase from Emericella nidulans showing hypertolerance to temperature and organic solvents , 2011 .

[23]  R. K. Saxena,et al.  Bacillus sphaericus: the highest bacterial tannase producer with potential for gallic acid synthesis. , 2011, Journal of bioscience and bioengineering.

[24]  P. Bottomley,et al.  Role of a Fur homolog in iron metabolism in Nitrosomonas europaea , 2011, BMC Microbiology.

[25]  H. S. El-Beltagi,et al.  Variations in fatty acid composition, glucosinolate profile and some phytochemical contents in selected oil seed rape (Brassica napus L.) cultivars , 2010 .

[26]  R. J. Green,et al.  Interactions of tea tannins and condensed tannins with proteins. , 2010, Journal of pharmaceutical and biomedical analysis.

[27]  Marie-Hélène Morel,et al.  Modification of pasta structure induced by high drying temperatures. Effects on the in vitro digestibility of protein and starch fractions and the potential allergenicity of protein hydrolysates , 2009 .

[28]  M. Ozacar,et al.  Competitive biosorption of Pb2+, Cu2+ and Zn2+ ions from aqueous solutions onto valonia tannin resin. , 2009, Journal of hazardous materials.

[29]  B. Turchetti,et al.  Antimicrobial and antiviral activity of hydrolysable tannins. , 2008, Mini reviews in medicinal chemistry.

[30]  R. Osawa,et al.  Identification and cloning of a gene encoding tannase (tannin acylhydrolase) from Lactobacillus plantarum ATCC 14917(T). , 2008, Systematic and applied microbiology.

[31]  Ö. Canbolat,et al.  Effects of NaOH treatment on condensed tannin contents and gas production kinetics of tree leaves , 2007 .

[32]  K. Hambidge,et al.  Phytate, zinc, iron and calcium content of selected raw and prepared foods consumed in rural Sidama, Southern Ethiopia, and implications for bioavailability , 2007 .

[33]  H. Makkar,et al.  Wood ash treatment, a cost-effective way to deactivate tannins in Acacia cyanophylla Lindl. foliage , 2005 .

[34]  D. Tomé,et al.  In vivo determination of amino acid bioavailability in humans and model animals. , 2005, Journal of AOAC International.

[35]  A. Kodjo,et al.  Enterotoxin D producing strains of Staphylococcus aureus are typeable by pulsed-field gel electrophoresis (PFGE) , 2005 .

[36]  M. Al-Mamary,et al.  In vivo effects of dietary sorghum tannins on rabbit digestive enzymes and mineral absorption , 2001 .

[37]  A. Pell,et al.  Analysis of condensed tannins: a review , 2001 .

[38]  Shweta Sharma,et al.  A spectrophotometric method for assay of tannase using rhodanine. , 2000, Analytical biochemistry.

[39]  K. Lacki,et al.  Decrease of tannin content in canola meal by an enzyme preparation from Trametes versicolor , 1996, Biotechnology Letters.

[40]  V. C. Nair,et al.  Reduction of phytic acid content in canola meal by Aspergillus ficuum in solid state fermentation process , 1990, Applied Microbiology and Biotechnology.

[41]  J. Melcion,et al.  Utilization of tannin-containing and tannin-free faba beans (Vicia faba) by young chicks: Effects of pelleting feeds on energy, protein and starch digestibility , 1988 .

[42]  J. M. Bell.  Nutrients and toxicants in rapeseed meal: a review. , 1984, Journal of animal science.

[43]  E. Ertaş,et al.  Change of enzyme activity and quality during the processing of Turkish green tea , 2016 .

[44]  A. A. Sherief,et al.  Brazilian Journal of Chemical Engineering CATALYTIC PROPERTIES OF IMMOBILIZED TANNASE PRODUCED FROM Aspergillus aculeatus COMPARED WITH THE FREE ENZYME , 2011 .

[45]  M. Tripathi,et al.  GLICOSINOLATES IN ANIMAL NUTRITION: A REVIEW , 2007 .

[46]  S. Al-Asheh,et al.  Production of phytase during solid state fermentation using Aspergillus ficuum NRRL 3135 in canola meal , 1995 .

[47]  M. Ragan,et al.  Phlorotannins, brown algal polyphenols , 1986 .