The potential of genetic engineering for improving brewing, wine-making and baking yeasts

Abstract. The end of the twentieth century was marked by major advances in life technology, particularly in areas related to genetics and more recently genomics. Considerable progress was made in the development of genetically improved yeast strains for the wine, brewing and baking industries. In the last decade, recombinant DNA technology widened the possibilities for introducing new properties. The most remarkable advances, which are discussed in this Mini-Review, are improved process performance, off-flavor elimination, increased formation of by-products, improved hygienic properties or extension of substrate utilization. Although the introduction of this technology into traditional industries is currently limited by public perception, the number of potential applications of genetically modified industrial yeast is likely to increase in the coming years, as our knowledge derived from genomic analyses increases.

[1]  C. Boone,et al.  Integration of the yeast K1 killer toxin gene into the genome of marked wine yeasts and its effect on vinification , 1990 .

[2]  N. Urano,et al.  Conversion of a non-flocculent brewer's yeast to flocculent ones by electrofusion , 1993 .

[3]  L. Wodicka,et al.  Genome-wide expression monitoring in Saccharomyces cerevisiae , 1997, Nature Biotechnology.

[4]  P. Barré,et al.  Glycerol Overproduction by Engineered Saccharomyces cerevisiae Wine Yeast Strains Leads to Substantial Changes in By-Product Formation and to a Stimulation of Fermentation Rate in Stationary Phase , 1999, Applied and Environmental Microbiology.

[5]  J. Hammond Genetically‐modified brewing yeasts for the 21st century. Progress to date , 1995, Yeast.

[6]  F. Zimmermann,et al.  Simultaneous overexpression of enzymes of the lower part of glycolysis can enhance the fermentative capacity of Saccharomyces cerevisiae , 2000, Yeast.

[7]  A. Querol,et al.  Construction of a recombinant wine yeast strain expressing beta-(1,4)-endoglucanase and its use in microvinification processes , 1993, Applied and environmental microbiology.

[8]  I. S. Pretorius,et al.  Co-expression of a Saccharomyces diastaticus glucoamylase-encoding gene and a Bacillus amyloliquefaciens alpha-amylase-encoding gene in Saccharomyces cerevisiae. , 1991, Gene.

[9]  I. S. Pretorius,et al.  Effect of Increased Yeast Alcohol Acetyltransferase Activity on Flavor Profiles of Wine and Distillates , 2000, Applied and Environmental Microbiology.

[10]  R. Needleman Control of maltase synthesis in yeast , 1991, Molecular microbiology.

[11]  P. Attfield,et al.  Genetic Evidence That High Noninduced Maltase and Maltose Permease Activities, Governed by MALx3-Encoded Transcriptional Regulators, Determine Efficiency of Gas Production by Baker’s Yeast in Unsugared Dough , 1999, Applied and Environmental Microbiology.

[12]  J. Hansen,et al.  Inactivation of MET10 in brewer's yeast specifically increases SO2 formation during beer production , 1996, Nature Biotechnology.

[13]  P. Sanz,et al.  Engineering baker's yeast: room for improvement. , 1999, Trends in biotechnology.

[14]  P. Barré,et al.  Examination of the transcriptional specificity of an enological yeast. A pilot experiment on the chromosome-III right arm , 2000, Current Genetics.

[15]  Naoto Urano,et al.  Conversion of a non-flocculent brewer's yeast to flocculent ones by electrofusion: 2. Small-scale brewing by fusants , 1993 .

[16]  I. S. Pretorius The Genetic Improvement of Wine Yeasts , 2003 .

[17]  M. Viljoen,et al.  Malolactic Fermentation in Grape Musts by a Genetically Engineered Strain of Saccharomyces cerevisiae , 1997, American Journal of Enology and Viticulture.

[18]  C. Ough,et al.  Urea Removal from Wine by an Acid Urease , 1988, American Journal of Enology and Viticulture.

[19]  I. S. Pretorius,et al.  Tailoring wine yeast for the new millennium: novel approaches to the ancient art of winemaking , 2000, Yeast.

[20]  F. Zimmermann,et al.  Overproduction of glycolytic enzymes in yeast , 1989, Yeast.

[21]  D. Gelfand,et al.  Stable Expression of Aspercillus Awamori Glucoamylase in Distiller's Yeast , 1988, Bio/Technology.

[22]  G. Stewart,et al.  One hundred years of yeast research and development in the brewing industry , 1986 .

[23]  C. A. Masschelein,et al.  Subthreshold Vicinal Diketone Levels in Lager Brewing Yeast Fermentations by Means of ILV5 Gene Amplification1 , 1990 .

[24]  K. Kondo,et al.  Application of a ribosomal DNA integration vector in the construction of a brewer's yeast having alpha-acetolactate decarboxylase activity , 1990, Applied and environmental microbiology.

[25]  A. Iwamatsu,et al.  Molecular cloning, sequence analysis, and expression of the yeast alcohol acetyltransferase gene , 1994, Applied and environmental microbiology.

[26]  Vesa Joutsjoki,et al.  Construction of a Stable α-Galactosidase-Producing Baker's Yeast Strain , 1988 .

[27]  P. Barré,et al.  Modulation of Glycerol and Ethanol Yields During Alcoholic Fermentation in Saccharomyces cerevisiae Strains Overexpressed or Disrupted for GPD1 Encoding Glycerol 3‐Phosphate Dehydrogenase , 1997, Yeast.

[28]  J. Gancedo,et al.  Futile cycles in Saccharomyces cerevisiae strains expressing the gluconeogenic enzymes during growth on glucose. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[29]  Ken-ichi Watanabe,et al.  Stable Overproduction of Isoamyl Alcohol by Saccharomyces cerevisiae with Chromosome-integrated Multicopy LEU4 Genes , 1992 .

[30]  P. Barré,et al.  Localization and cell surface anchoring of the Saccharomyces cerevisiae flocculation protein Flo1p , 1997, Journal of bacteriology.

[31]  I. S. Pretorius,et al.  The development of bactericidal yeast strains by expressing the Pediococcus acidilactici pediocin gene (pedA) in Saccharomyces cerevisiae , 1999, Yeast.

[32]  P. Aldhous Genetic engineering. Modified yeast fine for food. , 1990, Nature.

[33]  K. Ouchi,et al.  Role of the yeast maltose fermentation genes in CO2 production rate from sponge dough , 1990 .

[34]  P. Barré,et al.  Acidification of Grape Musts by Saccharomyces cerevisiae Wine Yeast Strains Genetically Engineered to Produce Lactic Acid , 1999, American Journal of Enology and Viticulture.

[35]  J. Hansen Inactivation of MXR1 Abolishes Formation of Dimethyl Sulfide from Dimethyl Sulfoxide inSaccharomyces cerevisiae , 1999, Applied and Environmental Microbiology.

[36]  P. Barré,et al.  Malolactic fermentation by engineered Saccharomyces cerevisiae as compared with engineered Schizosaccharomyces pombe , 1996, Yeast.

[37]  J. Hansen,et al.  Inactivation of MET2 in brewer's yeast increases the level of sulfite in beer. , 1996, Journal of biotechnology.

[38]  P. Sigsgaard,et al.  Towards diacetyl‐less brewers' yeast. Influence of ilv2 and ilv5 mutations , 1988, Journal of basic microbiology.

[39]  Carlo Zambonelli,et al.  Improvement of a Wine Saccharomyces cerevisiae Strain by a Breeding Program , 1985, Applied and environmental microbiology.

[40]  T. Benítez,et al.  Development of New Strains for the Food Industry , 1996 .

[41]  P. Barré,et al.  Purification, characterization, and substrate specificity of a novel highly glucose-tolerant beta-glucosidase from Aspergillus oryzae. , 1998, Applied and environmental microbiology.

[42]  T. Sasaki,et al.  Breeding of a Brewer's Yeast Possessing Anticontaminant Properties , 1984 .

[43]  G. Reed,et al.  Brewer’s Yeast , 1991 .

[44]  Sylvie Dequin,et al.  Engineering of the Pyruvate Dehydrogenase Bypass inSaccharomyces cerevisiae: Role of the Cytosolic Mg2+ and Mitochondrial K+ Acetaldehyde Dehydrogenases Ald6p and Ald4p in Acetate Formation during Alcoholic Fermentation , 2000, Applied and Environmental Microbiology.

[45]  P. Barré,et al.  Cloning, sequence and expression of the gene encoding the malolactic enzyme from Lactococcus lactis , 1993, FEBS letters.

[46]  W. H. Zyl,et al.  Over-expression of the Saccharomyces cerevisiae exo-β-1,3-glucanase gene together with the Bacillus subtilis endo-β-1,3-1,4-glucanase gene and the Butyrivibrio fibrisolvens endo-β-1,4-glucanase gene in yeast , 1997 .

[47]  K. Takahashi,et al.  Genetic engineering of a sake yeast producing no urea by successive disruption of arginase gene , 1991, Applied and environmental microbiology.

[48]  E. Aubertin [Brewer's yeast]. , 1951, Journal de medecine de Bordeaux et du Sud-Ouest.

[49]  T. Benítez,et al.  Characterization of genetically transformed Saccharomyces cerevisiae baker's yeasts able to metabolize melibiose , 1995, Applied and environmental microbiology.

[50]  M. Aigle,et al.  Cloning and sequence analysis of the gene encoding Lactococcus lactis malolactic enzyme: relationships with malic enzymes. , 1994, FEMS microbiology letters.

[51]  S. Mithieux,et al.  Tandem integration of multiple ILV5 copies and elevated transcription in polyploid yeast , 1995, Yeast.

[52]  M. Aigle,et al.  Functional expression in Saccharomyces cerevisiae of the Lactococcus lactis mleS gene encoding the malolactic enzyme. , 1995, FEMS microbiology letters.

[53]  P. Meaden,et al.  PROPERTIES OF A GENETICALLY‐ENGINEERED DEXTRIN‐FERMENTING STRAIN OF BREWERS' YEAST , 1988 .

[54]  S. Keränen,et al.  CONSTRUCTION OF FLOCCULENT BREWER'S YEAST BY CHROMOSOMAL INTEGRATION OF THE YEAST FLOCCULATION GENE FLO1 , 1994 .

[55]  T. Ogata,et al.  Chromosomal structures of bottom fermenting yeasts. , 1999, Systematic and applied microbiology.

[56]  K. Kitamoto,et al.  Brewing properties of sake yeast whose EST2 gene encoding isoamyl acetate-hydrolyzing esterase was disrupted , 1998 .

[57]  P. Langridge,et al.  Regulation of hydrogen sulfide liberation in wine-producing Saccharomyces cerevisiae strains by assimilable nitrogen , 1995, Applied and environmental microbiology.

[58]  M. Aigle,et al.  Development of a polymerase chain reaction/restriction fragment length polymorphism method for Saccharomyces cerevisiae and Saccharomyces bayanus identification in enology. , 1996, FEMS microbiology letters.

[59]  B. Barrell,et al.  Life with 6000 Genes , 1996, Science.

[60]  Luis González-Candelas,et al.  Construction of a recombinant wine yeast strain expressing a fungal pectate lyase gene. , 1995, FEMS microbiology letters.

[61]  B. Saha,et al.  Production, purification, and characterization of a highly glucose-tolerant novel beta-glucosidase from Candida peltata , 1996, Applied and environmental microbiology.

[62]  K. Kondo,et al.  Nucleotide sequence and expression of the Enterobacter aerogenes alpha-acetolactate decarboxylase gene in brewer's yeast , 1988, Applied and environmental microbiology.

[63]  P. Lehtovaara,et al.  Expression of two Trichoderma reesei endoglucanases in the yeast Saccharomyces cerevisiae , 1987, Yeast.

[64]  P. Attfield,et al.  Enhancement of maltose utilisation by Saccharomyces cerevisiae in medium containing fermentable hexoses , 1999, Journal of Industrial Microbiology and Biotechnology.

[65]  C. Ough Ethylcarbamate in fermented beverages and foods. I. Naturally occurring ethylcarbamate. , 1976, Journal of agricultural and food chemistry.

[66]  I. S. Pretorius,et al.  Yeast Stress Response and Fermentation Efficiency: How to Survive the Making of Wine - A Review , 2019, South African Journal of Enology & Viticulture.

[67]  S. Dequin,et al.  Glycerol export and glycerol-3-phosphate dehydrogenase, but not glycerol phosphatase, are rate limiting for glycerol production in Saccharomyces cerevisiae. , 2001, Metabolic engineering.

[68]  P. Brown,et al.  Exploring the metabolic and genetic control of gene expression on a genomic scale. , 1997, Science.

[69]  Y. Shibano,et al.  Reduction of hydrogen sulfide production in brewing yeast by constitutive expression of MET25 gene , 1995 .

[70]  Paul V. Attfield,et al.  Stress tolerance: The key to effective strains of industrial baker's yeast , 1997, Nature Biotechnology.

[71]  M. Stratford Yeast flocculation: Reconciliation of physiological and genetic viewpoints , 1992, Yeast.

[72]  P. Barré,et al.  Mixed Lactic Acid–Alcoholic Fermentation by Saccharomyes cerevisiae Expressing the Lactobacillus casei L(+)–LDH , 1994, Bio/Technology.

[73]  C. Kurtzman,et al.  Deoxyribonucleic acid relatedness among species of the genus Saccharomyces sensu stricto , 1985 .

[74]  E. Nevoigt,et al.  Reduced pyruvate decarboxylase and increased glycerol‐3‐phosphate dehydrogenase [NAD+] levels enhance glycerol production in Saccharomyces cerevisiae , 1996, Yeast.

[75]  R. Kuroki,et al.  Region of Flo1 Proteins Responsible for Sugar Recognition , 1998, Journal of bacteriology.

[76]  A. Querol,et al.  The application of molecular techniques in wine microbiology , 1996 .

[77]  E. Hinchliffe CAMBRIDGE PRIZE LECTURE DEVELOPING NEW STRAINS OF YEAST , 1992 .

[78]  M. Penttilä,et al.  Recombinant brewer's yeast strains suitable for accelerated brewing. , 1990, Journal of biotechnology.

[79]  Toshio Mori,et al.  Cloning of a gene suppressing hydrogen sulfide production by Saccharomyces cerevisiae and its expression in a brewing yeast , 1992 .