Rapid microassay of gentamicin, kanamycin, neomycin, streptomycin, and vancomycin in serum or plasma.

Abstract With slight modifications in the standard agar diffusion methods, it was possible to determine the concentrations of antibiotics in 0.08 ml. samples of serum or plasma. The mean error was ±11 per cent and the results were available after incubation for 112 to 312 hours at 37 °C. Gentamicin, kanamycin, streptomycin, neomycin, or vancomycin were rapidly assayed, even in the presence of penicillins or cephalosporins which could be readily inactivated with a zinc-requiring, "broad-spectrum" β-lactamase. The method was particularly helpful for regulation of the therapy in patients with diminished renal function in whom estimation of dosage on the basis of renal function was less reliable.

[1]  E. Abraham,et al.  The composition of β-lactamase I and β-lactamase II from Bacillus cereus 569/H , 1970 .

[2]  R. Cutler,et al.  Correlation of serum creatinine concentration and kanamycin half-life. Therapeutic implications. , 1969, JAMA.

[3]  M. Finland,et al.  Measurement of three antibiotics (penicillin, cephalothin, and chloramphenicol) when present together in mixtures. , 1968, Applied microbiology.

[4]  M. Finland,et al.  Thiol-Group Binding of Zinc to a β-Lactamase of Bacillus cereus: Differential Effects on Enzyme Activity with Penicillin and Cephalosporins as Substrates , 1968, Journal of bacteriology.

[5]  C. Kunin.  A guide to use of antibiotics in patients with renal disease. A table of recommended doses and factors governing serum levels. , 1967, Annals of internal medicine.

[6]  E. Abraham,et al.  A comparison of the action of penicillinase on benzylpenicillin and cephalosporin N and the competitive inhibition of penicillinase by cephalosporin C. , 1956, The Biochemical journal.

[7]  W. A. Randall,et al.  Assay Methods of Antibiotics a Laboratory Manual , 1956 .

[8]  M. Pollock,et al.  Purification of penicillin-induced penicillinase of Bacillus cereus NRRL 569: a comparison of its properties with those of a similarly purified penicillinase produced spontaneously by a constitutive mutant strain. , 1956, The Biochemical journal.

[9]  J. Morton,et al.  Filter Paper Disc Modification of the Oxford Cup Penicillin Determination.∗ , 1944 .

[10]  C. Rammelkamp,et al.  A Method for Determining the Concentration of Penicillin in Body Fluids and Exudates∗ , 1942 .

[11]  J. Foster.  QUANTITATIVE ESTIMATION OF PENICILLIN , 1942 .

[12]  Howard Florey,et al.  FURTHER OBSERVATIONS ON PENICILLIN , 1941 .

[13]  E. Abraham,et al.  Zinc as a cofactor for cephalosporinase from Bacillus cereus 569. , 1966, The Biochemical journal.

[14]  Sabath Ld,et al.  Cephalosporinase and penicillinase activity of Bacillus cereus. , 1965 .

[15]  Axline Sg,et al.  CLINICAL PHARMACOLOGY OF ANTIMICROBIALS IN PREMATURE INFANTS. I. KANAMYCIN, STREPTOMYCIN, AND NEOMYCIN. , 1964, Antimicrobial agents and chemotherapy.

[16]  N G Heatley,et al.  A method for the assay of penicillin. , 1944, The Biochemical journal.