Biodegradation of Crude Oil by Kocuria sp.

Oil fields are not uniformly dispensed on the world ’s map, but are limited in several areas such as Pe r ian Gulf region. Frequent international transport by tankers and shi pping accidents makes the oil and its products a mo st important pollutant of ecosystems. After these accidents, different met hods have been developed for clean-up of the pollut ed areas, out of which bioremediation has attracted highest attention. The present paper describes the crude oil degradation by bacteria Kocuria sp. 27/1, isolated from contaminated wastewater by oil hydrocarbons. This bacteria was used for inoculatio n of mineral salt medium containing 500; 1000; 2000; and 4000 ppm of crude oil (v/v). Incubation was performed in orbita l shaker (250 rpm) at 30°C for 7 days. The optical density of the liqui d culture and final oil concentration were measured . Results showed that Kocuria sp. 27/1 was capable of crude oil degradation. Throug h t the experiment, the optical density was rapidl y increased comparing to the control. The results obtained by t his research showed that Kocuria sp. 27/1 can potentially be used for application in crude oil biodegradation of natural environments.

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