Graphene and gold nanoparticles based reagentless biodevice for phenolic endocrine disruptors monitoring

Abstract The goal of this paper aimed the development of an inexpensive, reliable and easy-to-use biodevice for detection and monitoring of phenol and phenolic endocrine disruptors in water samples. X-Ray (XRD) diffraction technique was employed for physical characterization of the modified electrode surface, results revealing nanostructured layers assemblies of polycrystalline gold with (200) growth preferred orientation and gold crystallite size of 33.19 nm. Investigation of the modified surface charge transfer properties was performed using cyclic voltammetry technique highlighting a significant enhancement of the electron transfer rate. Dual signal amplification offered by synergistic effect of gold nanoparticle and reduced graphene oxide layers and tyrosinase led to competitive detection limits (7.2 × 10− 8 mol L− 1for phenol and 4.8 × 10− 7 mol L− 1 for octylphenol) and sensitivities (416 nA/μmol for phenol and 155 nA/μmol for octylphenol). The obtained values of the Kmapp and Imax/Kmapp ratio confirmed a strongly dependence of the immobilized tyrosinase catalytic efficiency on the steric and electronic properties of the bulky side chain in the para position of the phenolic compound. The biodevice showed a percent recovery between 87 ± 8% and 94 ± 11% demonstrating a suitable degree of accuracy and confirming the application potential to the detection and monitoring of phenol and several endocrine disruptors in water samples.

[1]  R. Fernández-Torres,et al.  A novel application of three phase hollow fiber based liquid phase microextraction (HF-LPME) for the HPLC determination of two endocrine disrupting compounds (EDCs), n-octylphenol and n-nonylphenol, in environmental waters. , 2013, The Science of the total environment.

[2]  R Cela,et al.  Determination of parabens and triclosan in indoor dust using matrix solid-phase dispersion and gas chromatography with tandem mass spectrometry. , 2007, Analytical chemistry.

[3]  A. Dinescu,et al.  l-Lactic acid biosensor based on multi-layered graphene , 2013, Journal of Applied Electrochemistry.

[4]  A. Messina,et al.  Phenols removal by immobilized tyrosinase reactor in on-line high performance liquid chromatography. , 2006, Analytica chimica acta.

[5]  Terri Damstra,et al.  International Programme on Chemical Safety Global Assessment: The State-of-the-Science of Endocrine Disruptors , 2002 .

[6]  Michael Thompson,et al.  Harmonized guidelines for single-laboratory validation of methods of analysis (IUPAC Technical Report) , 2002 .

[7]  Lourdes Rivas,et al.  Iridium oxide nanoparticle induced dual catalytic/inhibition based detection of phenol and pesticide compounds. , 2014, Journal of materials chemistry. B.

[8]  Chao-ying Wang,et al.  A molecularly imprinted electrochemical sensor based on sol–gel technology and multiwalled carbon nanotubes–Nafion functional layer for determination of 2-nonylphenol in environmental samples , 2014 .

[9]  Lo Gorton,et al.  Carbon paste electrodes modified with enzymes, tissues, and cells , 1995 .

[10]  M. Diaconu,et al.  Modulating indium doped tin oxide electrode properties for laccase electron transfer enhancement , 2014 .

[11]  G. Swain,et al.  Comparison of the Electrical, Optical, and Electrochemical Properties of Diamond and Indium Tin Oxide Thin-Film Electrodes , 2005 .

[12]  J. Munoz-Munoz,et al.  Phenolic substrates and suicide inactivation of tyrosinase: kinetics and mechanism. , 2008, The Biochemical journal.

[13]  A. Dinescu,et al.  Isocyanate functionalized graphene/P3HT based nanocomposites , 2013 .

[14]  K. Ballschmiter,et al.  Determination of endocrine-disrupting phenolic compounds and estrogens in surface and drinking water by HRGC-(NCI)-MS in the picogram per liter range. , 2001, Environmental science & technology.

[15]  I. David Disposable carbon electrodes as an alternative for the direct voltammetric determination of alkyl phenols from water samples , 2013 .

[16]  D. Matějíček Multi heart-cutting two-dimensional liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometry method for the determination of endocrine disrupting compounds in water. , 2012, Journal of chromatography. A.

[17]  Younan Xia,et al.  One‐Dimensional Nanostructures: Synthesis, Characterization, and Applications , 2003 .

[18]  G. Doyle Community strategy for endocrine disrupters , 2014 .

[19]  J. S. Gutkind,et al.  Electrochemical Immunosensors for Interleukin-6. Comparison of Carbon Nanotube Forest and Gold Nanoparticle platforms. , 2009, Electrochemistry communications.

[20]  Sanjeev Kumar,et al.  Modeling of Formation of Gold Nanoparticles by Citrate Method , 2007 .

[21]  Arben Merkoçi,et al.  Bismuth nanoparticles for phenolic compounds biosensing application. , 2013, Biosensors & bioelectronics.

[22]  C. Bala,et al.  Sensitive detection of endocrine disrupters using ionic liquid--single walled carbon nanotubes modified screen-printed based biosensors. , 2011, Talanta.

[23]  R. Compton,et al.  The Voltammetry and Electroanalysis of Some Estrogenic Compounds at Modified Diamond Electrodes , 2013 .

[24]  Munetaka Oyama,et al.  A novel electrode surface fabricated by directly attaching gold nanospheres and nanorods onto indium tin oxide substrate with a seed mediated growth process , 2004 .

[25]  R. Compton,et al.  The use of nanoparticles in electroanalysis: a review , 2006, Analytical and bioanalytical chemistry.

[26]  L. Mita,et al.  A thionine-modified carbon paste amperometric biosensor for catechol and bisphenol A determination. , 2010, Biosensors & bioelectronics.

[27]  Sangjin Park,et al.  Amperometric immunosensing using an indium tin oxide electrode modified with multi-walled carbon nanotube and poly(ethylene glycol)-silane copolymer. , 2007, Chemical communications.

[28]  D. Barceló,et al.  Determination of 13 estrogenic endocrine disrupting compounds in atmospheric particulate matter by pressurised liquid extraction and liquid chromatography-tandem mass spectrometry , 2013, Analytical and Bioanalytical Chemistry.

[29]  N. Mohamed,et al.  Electrochemical Deposition of Gold Nanoparticles on Pencil Graphite by Fast Scan Cyclic Voltammetry , 2011 .

[30]  M. L. Mena,et al.  Development of a tyrosinase biosensor based on gold nanoparticles-modified glassy carbon electrodes: Application to the measurement of a bioelectrochemical polyphenols index in wines , 2005 .

[31]  M. Rebelo,et al.  An amperometric biosensor for polyphenolic compounds in red wine. , 2004, Biosensors & bioelectronics.

[32]  Lia Stanciu,et al.  Enzyme functionalized nanoparticles for electrochemical biosensors: a comparative study with applications for the detection of bisphenol A. , 2010, Biosensors & bioelectronics.

[33]  Rafael Rodríguez-Amaro,et al.  New Biosensor for Phenols Compounds Based on Gold Nanoparticle-Modified PVC/TTF-TCNQ Composite Electrode , 2012 .

[34]  Wu Yang,et al.  A novel electrochemical sensor of bisphenol A based on stacked graphene nanofibers/gold nanoparticles composite modified glassy carbon electrode , 2013 .

[35]  J. K. Bewtra,et al.  Enzyme‐Catalyzed Removal of Phenol from Refinery Wastewater: Feasibility Studies , 2001, Water environment research : a research publication of the Water Environment Federation.

[36]  R. Yu,et al.  A novel tyrosinase biosensor based on hydroxyapatite-chitosan nanocomposite for the detection of phenolic compounds. , 2010, Analytica chimica acta.

[37]  Carmen C. Mayorga-Martinez,et al.  Electrocatalytic tuning of biosensing response through electrostatic or hydrophobic enzyme-graphene oxide interactions. , 2014, Biosensors & bioelectronics.

[38]  H. Neels,et al.  Simultaneous determination of bisphenol A, triclosan, and tetrabromobisphenol A in human serum using solid-phase extraction and gas chromatography-electron capture negative-ionization mass spectrometry , 2008, Analytical and bioanalytical chemistry.

[39]  Andre K. Geim,et al.  The rise of graphene. , 2007, Nature materials.

[40]  Sang Yup Lee,et al.  Solution chemistry of self-assembled graphene nanohybrids for high-performance flexible biosensors. , 2010, ACS nano.

[41]  H. Kuramitz,et al.  Electrochemical oxidation of bisphenol A. Application to the removal of bisphenol A using a carbon fiber electrode. , 2001, Chemosphere.

[42]  A. Zgoła-Grześkowiak Dispersive liquid-liquid microextraction applied to isolation and concentration of alkylphenols and their short-chained ethoxylates in water samples. , 2010, Journal of chromatography. A.

[43]  A. Radoi,et al.  Disposable biosensor based on platinum nanoparticles-reduced graphene oxide-laccase biocomposite for the determination of total polyphenolic content. , 2013, Talanta.

[44]  R. Webster,et al.  Electrochemical Oxidation of Bisphenol A , 2013 .

[45]  Lauro T. Kubota,et al.  Electrochemical biosensor-based devices for continuous phenols monitoring in environmental matrices , 2002 .

[46]  G. Swain,et al.  Total inorganic arsenic detection in real water samples using anodic stripping voltammetry and a gold-coated diamond thin-film electrode. , 2007, Analytica chimica acta.

[47]  Damià Barceló,et al.  Monitoring of estrogens, pesticides and bisphenol A in natural waters and drinking water treatment plants by solid-phase extraction-liquid chromatography-mass spectrometry. , 2004, Journal of chromatography. A.