Evaluation of dietary Pancreatin as an exogenous enzyme on growth performance, gene expression, immunological responses, serum immunoglobins, and intestinal morphology in cockerels

ABSTRACT The current study evaluated the inclusion of Pancreatin enzyme on growth performance, intestinal morphology, endogenous enzyme activity, and immunological responses in cockerels. A total of 480 d-old-Hy-line cockerels were randomly divided into 5 treatments, with 6 replicates, 16 birds per cage. Birds were given a standard corn-soybean-based (CD) starter and grower diet. Exogenous Pancreatin enzyme was supplemented at 0: 250; 500; 750, and 1000 mg/kg. Results demonstrated that Pancreatin supplementation did not affect (P > 0.05) the growth performance and duodenal enzyme activity of birds. However, the addition of Pancreatin enzyme at 500 mg/kg increased (P < 0.05) jejunal villus height at 42 d; duodenal crypt depth and jejunal crypt depth at 70 d. Pancreatin supplementation except 1000 mg/kg increased (P < 0.05) serum IgM but not IgA and IgG. Furthermore, Pancreatin supplementation had the potential to decrease jejunal pH, spleen gene expression, and antibodies titers against NDV. In conclusion, Pancreatin enzyme inclusion had no effect on cockerels’ growth performance despite the variation found on the gut morphology. Pancreatin enzyme at intermediary amounts (500 and 750 mg/kg) showed a satisfactory serum immunoglobulin result but had the potential to modulate differently on the antibody titers against NDV and gene expression.

[1]  A. Jabbar,et al.  Impact of Microbial Protease Enzyme and Dietary Crude Protein Levels on Growth and Nutrients Digestibility in Broilers over 15–28 Days , 2021, Animals : an open access journal from MDPI.

[2]  A. Jabbar,et al.  Interactive effect of exogenous protease enzyme and dietary crude protein levels on growth and digestibility indices in broiler chickens during the starter phase , 2020, Tropical Animal Health and Production.

[3]  O. Adeola,et al.  Intestinal starch and energy digestibility in broiler chickens fed diets supplemented with α-amylase , 2020, Poultry science.

[4]  A. Swelum,et al.  Growth, carcass characteristics, and meat quality of broilers fed a low-energy diet supplemented with a multienzyme preparation , 2020, Poultry science.

[5]  Jun Jiang,et al.  Dietary Supplementation with Phytase and Protease Improves Growth Performance, Serum Metabolism Status, and Intestinal Digestive Enzyme Activities in Meat Ducks , 2020, Animals : an open access journal from MDPI.

[6]  A. Hafeez,et al.  Effect of diet supplemented with phytogenics and protease enzyme on performance, serum biochemistry and muscle histomorphology in broilers , 2020 .

[7]  Y. Attia,et al.  Effect of Different Levels of Multienzymes on Immune Response, Blood Hematology and Biochemistry, Antioxidants Status and Organs Histology of Broiler Chicks Fed Standard and Low-Density Diets , 2020, Frontiers in Veterinary Science.

[8]  N. El-Desoky,et al.  Effects of Moringa oleifera extracts and monensin on performance of growing rabbits , 2019, Livestock Science.

[9]  S. U. Rahman,et al.  Effect of different cereal-based diets supplemented with multi-enzyme blend on growth performance villus structure and gene expression (SGLT1, GLUT2, PepT1 and MUC2) in the small intestine of broiler chickens , 2019, Gene Reports.

[10]  A. Cowieson,et al.  Contribution of exogenous enzymes to potentiate the removal of antibiotic growth promoters in poultry production , 2019, Animal Feed Science and Technology.

[11]  S. Lewis,et al.  Structure and function of the immune system in the spleen , 2019, Science Immunology.

[12]  C. Walk,et al.  Evaluation of Phytase, Xylanase, and Protease in Reduced Nutrient Diets Fed to Broilers , 2019, Journal of Applied Poultry Research.

[13]  M. Emam,et al.  Exogenous dietary enzyme formulations improve growth performance of broiler chickens fed a low-energy diet targeting the intestinal nutrient transporter genes , 2018, PloS one.

[14]  B. Chae,et al.  The microbial pH-stable exogenous multienzyme improved growth performance and intestinal morphology of weaned pigs fed a corn–soybean-based diet , 2018 .

[15]  H. Ogata,et al.  Supplementation of pancreatic digestive enzymes alters the composition of intestinal microbiota in mice. , 2018, Biochemical and biophysical research communications.

[16]  A. Singh,et al.  Effect of a combination of xylanase, amylase and protease on growth performance of broilers fed low and high fiber diets , 2017 .

[17]  W. Jun,et al.  Effects of dietary protein levels and multienzyme supplementation on growth performance and markers of gut health of broilers fed a miscellaneous meal based diet , 2017 .

[18]  Lin Yuan,et al.  Effects of protease and non-starch polysaccharide enzyme on performance, digestive function, activity and gene expression of endogenous enzyme of broilers , 2017, PloS one.

[19]  M. Kudo,et al.  Immunopathogenesis of pancreatitis , 2016, Mucosal Immunology.

[20]  A. Seidavi,et al.  Effect of olive meal and supplemental enzymes on performance traits, blood biochemistry, humoral immunity response and caecal microbiota of broilers , 2017 .

[21]  A. Seidavi,et al.  Effects of dietary inclusion level of a mixture of probiotic cultures and enzymes on broiler chickens immunity response , 2017, Environmental Science and Pollution Research.

[22]  Xiao‐qiu Zhou,et al.  Impact of exogenous lipase supplementation on growth, intestinal function, mucosal immune and physical barrier, and related signaling molecules mRNA expression of young grass carp (Ctenopharyngodon idella). , 2016, Fish & shellfish immunology.

[23]  A. Taghizadeh,et al.  Effects of a multi-enzyme on performance and carcass characteristics of broiler chickens fed corn-soybean meal basal diets with different metabolizable energy levels , 2015 .

[24]  T. Woyengo,et al.  Enzymes enhance degradation of the fiber-starch-protein matrix of distillers dried grains with solubles as revealed by a porcine in vitro fermentation model and microscopy. , 2015, Journal of animal science.

[25]  B. Ding,et al.  The effects of enzyme supplementation on performance and digestive parameters of broilers fed corn-soybean diets. , 2014, Poultry science.

[26]  B. Slominski,et al.  The effect of protease, amylase, and nonstarch polysaccharide-degrading enzyme supplementation on nutrient utilization and growth performance of broiler chickens fed corn-soybean meal-based diets. , 2014, Poultry science.

[27]  B. Svihus.  Function of the digestive system , 2014 .

[28]  W. Kim,et al.  Comparative Apparent Metabolizable Energy Values of Soaking, Fermentation with Lactobacillus and Enzyme Treatment of Barley in Cockerels and Quails , 2013 .

[29]  P. Utterback,et al.  Comparative effects of dietary carbohydrases without or with protease on the ileal digestibility of energy and amino acids and AMEn in young broilers , 2013 .

[30]  F. Romeroa,et al.  Comparative effects of dietary carbohydrases without or with protease on the ileal digestibility of energy and amino acids and AMEn in young broilers , 2013 .

[31]  A. Abudabos.  Effect of Enzyme Supplementation to Normal and Low Density Broiler Diets Based on Corn-soybean Meal , 2012 .

[32]  R. Zijlstra,et al.  Ileal digestibility of amino acids, phosphorus, phytate and energy in pigs fed sorghum-based diets supplemented with phytase and Pancreatin®. , 2011, Journal of animal physiology and animal nutrition.

[33]  N. Baumgarth.  The double life of a B-1 cell: self-reactivity selects for protective effector functions , 2011, Nature Reviews Immunology.

[34]  J. Botermans,et al.  The growth of exocrine pancreatic insufficient young pigs fed an elemental diet is dependent on enteral pancreatin supplementation , 2010 .

[35]  Z. R. Xu,et al.  Effect of β-Mannanase (Hemicell) on Growth Performance and Immunity of Broilers , 2006 .

[36]  B. Faivre,et al.  The energetic cost of humoral immunity in the Collared Dove, Streptopelia decaocto: is the magnitude sufficient to force energy‐based trade‐offs? , 2005 .

[37]  D. Graham.  Pancreatic enzyme replacement , 1982, Digestive Diseases and Sciences.

[38]  D. Kil,et al.  Influences of Enzyme complex supplementation on growth, ileal and apparent fecal digestibility and morphology of small intestine in pigs , 2004 .

[39]  P. Iji,et al.  Intestinal function and body growth of broiler chickens on diets based on maize dried at different temperatures and supplemented with a microbial enzyme. , 2003, Reproduction, nutrition, development.

[40]  M. Nishibori,et al.  Cell-mediated and humoral immunity and phagocytic ability in chicken Lines divergently selected for serum immunoglobulin M and G levels. , 2000, Poultry science.

[41]  S. Leeson,et al.  Evaluation of dietary supplements of lipase, detergent, and crude porcine pancreas on fat utilization by young broiler chicks. , 1999, Poultry science.

[42]  E. Lhoste,et al.  Effects of dietary proteins on some pancreatic mRNAs encoding digestive enzymes in the pig , 1993 .