Assessment of the potential effect of some streams properties on the isolated Aeromonas hydrophila strains susceptibility against some β-Lactams and Sulfamids

The isolation and characterization of A. hydrophila strains were carried out a given stream located in the equatorial zone in Cameroon (Central Africa), during the mild rainy season and the mild dry season. The water physicochemical parameters measured were temperature, pH, electrical conductivity, color, turbidity and suspended solids. It has been noted that bacterial abundances as well as the values of physicochemical parameters undergoes temporal variations. Antimicrobial susceptibility tests showed that the means diameters of inhibition with Oxacillin, Ceftriazone, Penicellin, Sulfamethoxazol-trimethoprim, Chloramphenicol, Imipenem, Amoxicillin-Clavulanic acid and Cefazolin were 6.44, 21, 6.63, 9.58, 15.37, 22.18, 14.29 and 10.26 mm respectively. 100% of strains tested were resistant to Penicillin, 89.47 % resistant to Oxacillin, 68.42% resistant to Sulphamethoxazole-trimetroprim and 63.15% were resistant to Cefazolin. There were 54.60% cases of antibiotic resistance, 24.34 % of intermediate sensitivity and 21.05% of sensitive strains against the tested antibiotics. The encoded redundancy analysis indicates that the percentage of the variation explained on the canonical axes is of 47.8% for the F1 axis and 25.9% for the F2 axis, for an accumulated percentage of 73.7%. The suspended solids, the Ceftriazon and Chloramphenicol are correlated to the F1 axis in positive coordinates, and the Temperature and Amoxicillin-Clavulanic acid in negative coordinates. The correlation test between the water physicochemical characteristics and the inhibition diameters of antibiotics showed some significant correlations (P<0.05). The regulation of bacterial genes would be regulated by complex mechanisms. Although many factors implied are linked to the bacterial cell, others may belong to the closed environment.

[1]  Andreas Wagner,et al.  Growth Temperature and Genome Size in Bacteria Are Negatively Correlated, Suggesting Genomic Streamlining During Thermal Adaptation , 2013, Genome biology and evolution.

[2]  I. Gołaś,et al.  Tetracycline-resistant bacteria as indicators of antimicrobial resistance in protected waters—The example of the Drwęca River Nature Reserve (Poland) , 2011 .

[3]  I. Stojanov,et al.  Susceptibility of Aeromonas hydrophila isolates to antimicrobial drugs. , 2010 .

[4]  A. Abulhamd.  Genetic Diversity and Antimicrobial Susceptibility of Motile Aquatic Aeromonads , 2010 .

[5]  J. Ramalivhana,et al.  Antimicrobial susceptibility testing of Aeromonas hydrophila isolated from Limpopo Province, South Africa using VITEK 2 system, Micro Scan WalkAway, disk diffusion and E-test method , 2009 .

[6]  R. Mraouna,et al.  Effect of Previous Incubation of Aeromonas hydrophila in Wastewater Prior to Its Transfer Into Marine Water Microcosms , 2008 .

[7]  P. Dersch,et al.  Regulatory elements implicated in the environmental control of invasin expression in enteropathogenic Yersinia. , 2007, Advances in experimental medicine and biology.

[8]  L. A. Amaral,et al.  Bacteria of the genus Aeromonas in different locations throughout the process line of beef slaughtering Bactérias do gênero Aeromonas em diferentes pontos do fluxograma de abate bovino , 2006 .

[9]  M. Marceau.  Transcriptional regulation in Yersinia: an update. , 2005, Current issues in molecular biology.

[10]  J. Kalff,et al.  Factors controlling bacterial production in marine and freshwater sediments , 1993, Microbial Ecology.

[11]  J. Guarro,et al.  Characterisation of Aeromonas spp. isolated from frozen fish intended for human consumption in Mexico. , 2003, International journal of food microbiology.

[12]  M. Nola,et al.  Faecal coliforms and faecal streptococci community in the underground water in an equatorial area in Cameroon (Central Africa): the importance of some environmental chemical factors. , 2002, Water research.

[13]  M. Rybak,et al.  Effect of growth phase and pH on the in vitro activity of a new glycopeptide, oritavancin (LY333328), against Staphylococcus aureus and Enterococcus faecium. , 2002, The Journal of antimicrobial chemotherapy.

[14]  M. Ferrándiz,et al.  The promoter of the operon encoding the F0F1 ATPase of Streptococcus pneumoniae is inducible by pH , 2001, Molecular microbiology.

[15]  P. Caumette,et al.  Antimicrobial resistance of mesophilic Aeromonas spp. isolated from two European rivers. , 2000, The Journal of antimicrobial chemotherapy.

[16]  A. Abbott.  One for all — and all for one , 2000, Nature.

[17]  N. Yee,et al.  Experimental study of the pH, ionic strength, and reversibility behavior of bacteria–mineral adsorption , 2000 .

[18]  K. Tilly,et al.  Temperature-regulated expression of bacterial virulence genes. , 2000, Microbes and infection.

[19]  K. olańczuk-Neyman,et al.  The role of physico-chemical and biological processes in manganese and ammonia nitrogen removal from groundwater , 2000 .

[20]  T. Klaenhammer,et al.  Identification of the pH‐inducible, proton‐translocating F1F0‐ATPase (atpBEFHAGDC) operon of Lactobacillus acidophilus by differential display: gene structure, cloning and characterization , 1999, Molecular microbiology.

[21]  R. Hurme,et al.  Temperature sensing in bacterial gene regulation — what it all boils down to , 1998, Molecular microbiology.

[22]  H. Harms,et al.  Quantification of Polymer Interactions in Bacterial Adhesion , 1998 .

[23]  M. Hamze,et al.  SENSIBILITE A 23 ANTIBIOTIQUES DE 83 SOUCHES DE AEROMONAS HYDROPHILA ISOLEES D'EAUX LIBANAISES , 1998 .

[24]  H. Sørum,et al.  Transfer of multiple drug resistance plasmids between bacteria of diverse origins in natural microenvironments , 1994, Applied and environmental microbiology.

[25]  E. Olson.  Influence of pH on bacterial gene expression , 1993, Molecular microbiology.

[26]  J. Pelmont.  BACTÉRIES ET ENVIRONNEMENT ADAPTATIONS PHYSIOLOGIQUES , 1993 .

[27]  W. Wiebe,et al.  Bacterial Growth in the Cold: Evidence for an Enhanced Substrate Requirement , 1992, Applied and environmental microbiology.

[28]  M. Peterz,et al.  Enterotoxigenicity and drug sensitivity of Aeromonas hydrophila isolated from well water in Sweden: a case study. , 1989, International journal of food microbiology.

[29]  Georges Bachelier.  Etude pédologique des sols de Yaoundé : Contribution à l'étude de la pédogenèse des sols ferrallitiques , 1959 .

[30]  S. T. Cowan.  Bergey's Manual of Determinative Bacteriology , 1948, Nature.