A single amino acid substitution in the V1 loop of human immunodeficiency virus type 1 gp120 alters cellular tropism

Specific point mutations which affect viral tropism have been identified in both the V3 loop and in the CD4-binding region of the human immunodeficiency virus type 1 surface glycoprotein gp120. Here we report that a single point mutation in the first variable region (V1) of human immunodeficiency virus type 1 strain JRCSF is responsible for a change in viral tropism.

[1]  A J Langlois,et al.  Principal neutralizing domain of the human immunodeficiency virus type 1 envelope protein. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[2]  A. Cann,et al.  Human immunodeficiency virus type 1 T-cell tropism is determined by events prior to provirus formation , 1990, Journal of virology.

[3]  D. Baltimore,et al.  Factors affecting cellular tropism of human immunodeficiency virus , 1990, Journal of virology.

[4]  Robin A. Weiss,et al.  The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brain , 1986, Cell.

[5]  T. Masuda,et al.  Isolation of simian immunodeficiency virus from african green monkeys and seroepidemiologic survey of the virus in various non‐human primates , 1988, International journal of cancer.

[6]  A. Cann,et al.  The region of the envelope gene of human immunodeficiency virus type 1 responsible for determination of cell tropism , 1992, Journal of virology.

[7]  C. Patience,et al.  Assessment of Compounds for Anti-HIV Activity. , 1992, Methods in molecular biology.

[8]  Michael Emerman,et al.  Single amino-acid changes in HIV envelope affect viral tropism and receptor binding , 1989, Nature.

[9]  E K Ross,et al.  Functional interaction of constant and variable domains of human immunodeficiency virus type 1 gp120 , 1989, Journal of virology.

[10]  J. Sodroski,et al.  Characterization of a discontinuous human immunodeficiency virus type 1 gp120 epitope recognized by a broadly reactive neutralizing human monoclonal antibody , 1991, Journal of virology.

[11]  Luc Montagnier,et al.  T-lymphocyte T4 molecule behaves as the receptor for human retrovirus  LAV , 1984, Nature.

[12]  J. Levy,et al.  Differential ability of human immunodeficiency virus isolates to productively infect human cells. , 1987, Journal of immunology.

[13]  M. Greaves,et al.  The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus , 1984, Nature.

[14]  B. Cullen,et al.  Identification of the envelope V3 loop as the primary determinant of cell tropism in HIV-1. , 1991, Science.

[15]  C. Cheng‐Mayer,et al.  Macrophage and T cell-line tropisms of HIV-1 are determined by specific regions of the envelope gp!20 gene , 1991, Nature.

[16]  H. Vinters,et al.  Dual infection of the central nervous system by AIDS viruses with distinct cellular tropisms. , 1987, Science.

[17]  S. M. Stearns,et al.  Analysis of mutations in the V3 domain of gp160 that affect fusion and infectivity , 1992, Journal of virology.

[18]  G. Shaw,et al.  Macrophage tropism determinants of human immunodeficiency virus type 1 in vivo , 1992, Journal of virology.

[19]  Q. Sattentau,et al.  The CD4 antigen: Physiological ligand and HIV receptor , 1988, Cell.

[20]  R. F. Smith,et al.  Human Retroviruses and AIDS. A compilation and analysis of nucleic acid and amino acid sequences: I--II; III--V , 1993 .

[21]  M. Akutsu,et al.  Host range mutant of human immunodeficiency virus type 1: modification of cell tropism by a single point mutation at the neutralization epitope in the env gene , 1991, Journal of virology.

[22]  J Overbaugh,et al.  Variation in simian immunodeficiency virus env is confined to V1 and V4 during progression to simian AIDS , 1991, Journal of virology.