论文信息 - Automatic particle picking from electron micrographs.

Automatic particle picking from electron micrographs.

A computer program for automatic particle picking based on textural methods is proposed. The technique relies on the evaluation of certain textural parameters for data windows containing single particles, and those containing undesirable material. These parameters are manipulated by a discriminant analysis routine for determining the rules of classification between the different categories. The effectiveness of the method was demonstrated by application to electron micrographs of 70S Escherichia coli ribosomes.

[1] M. Radermacher,et al. Three-dimensional reconstruction of single particles from random and nonrandom tilt series. , 1988, Journal of electron microscopy technique.

[2] J. Frank,et al. The ribosome at improved resolution: new techniques for merging and orientation refinement in 3D cryo-electron microscopy of biological particles. , 1994, Ultramicroscopy.

[3] Jorge L. C. Sanz,et al. Radon and projection transform-based computer vision , 1988 .

[4] Joachim Frank,et al. SPIDER—A modular software system for electron image processing , 1981 .

[5] G Harauz,et al. Automatic selection of macromolecules from electron micrographs by component labelling and symbolic processing. , 1989, Ultramicroscopy.

[6] Joachim Frank,et al. Automatic selection of molecular images from electron micrographs , 1983 .

[7] Michael Spann,et al. Texture feature performance for image segmentation , 1990, Pattern Recognit..