Lectin-binding in premalignant lesions during submandibular gland carcinogenesis.

Complex carbohydrates in premalignant lesions of mouse submandibular gland tumors were examined by the lectin-peroxidase conjugate method. Peroxidase-conjugated lectins of PNA, RCA-1, DBA, SBA, UEA-1 and WGA were used to detect specific sugar residues of complex carbohydrates in premalignant lesions during experimental carcinogenesis. Marked reduction of PNA and SBA bindings occurred in duct-like structures and cystic lesions which were transformed from granular convoluted tubule cells. Premalignant lesions bound slightly to PNA, RCA-1, DBA, SBA and WGA and manifested increased UEA-1 binding. Squamous metaplastic epithelia of premalignant lesions manifested increased binding to PNA, RCA-1 and SBA as compared to those of duct-like structure and cystic epithelia.

[1]  Y. Takai,et al.  Histochemical studies on Concanavalin A-binding in experimental carcinoma of the mouse submandibular gland. , 1984, Journal of oral pathology.

[2]  Y. Takai,et al.  Histopathologic studies of DMBA-induced mouse submandibular-gland tumors with or without cryoprobe treatment. , 1984, Journal of oral pathology.

[3]  Y. Takai,et al.  USE OF LECTINS FOR DIFFERENTIAL LOCALIZATION OF SECRETORY MATERIALS OF GRANULAR CONVOLUTED TUBULES AND DUCTS IN THE SUBMANDIBULAR GLAND , 1983 .

[4]  B. Ponder.  8 – Lectin Histochemistry , 1983 .

[5]  M. Sarras,et al.  Regional differences in lectin binding to colonic epithelium by fluorescent and electron microscopy. , 1982, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[6]  T. Muramatsu,et al.  A new fucosyl antigen expressed on colon adenocarcinoma and embryonal carcinoma cells , 1982, Nature.

[7]  J. Lafitte,et al.  Use of lectins for detection of glycoconjugates in the glandular cells of the human bronchial mucosa. , 1982, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[8]  S. Hakomori,et al.  Isolation and characterization of a new fucoganglioside accumulated in precancerous rat liver and in rat hepatoma induced by N-2-acetylaminofluorene. , 1982, The Journal of biological chemistry.

[9]  T. Faraggiana,et al.  Lectin-peroxidase conjugate reactivity in normal human kidney. , 1982, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[10]  G. Strecker,et al.  Specificity of Twelve Lectins Towards Oligosaccharides and Glycopeptides Related to N‐Glycosylproteins , 2005 .

[11]  M. Etzler,et al.  DIFFERENTIAL LOCALIZATION OF CELL SURFACE AND SECRETORY COMPONENTS IN RAT INTESTINAL EPITHELIUM BY USE OF LECTINS , 1974, The Journal of cell biology.

[12]  N. Sharon,et al.  Lectins: cell-agglutinating and sugar-specific proteins. , 1972, Science.

[13]  S. Spicer,et al.  The histochemistry of carbohydrate‐rich substances in certain ungulate salivary glands , 1967, The Anatomical record.

[14]  I. Goldstein,et al.  PROTEIN-CARBOHYDRATE INTERACTION. II. INHIBITION STUDIES ON THE INTERACTION OF CONCANAVALIN A WITH POLYSACCHARIDES. , 1965, Biochemistry.

[15]  S. Spicer,et al.  Histochemical characteristics of mucopolysaccharides in salivary and exorbital lacrimal glands , 1964, The Anatomical record.

[16]  J. Shackleford,et al.  Structure and carbohydrate histochemistry of mammalian salivary glands. , 1962, The American journal of anatomy.