Plasmid Content of Some Oral Microorganisms Isolated from Subgingival Plaque

Eighty-five strains of bacterial species selected from the predominant cultivable dental plaque flora of patients with different periodontal pathologies were examined for their plasmid content. Microorganisms studied included: Actinomyces viscosus, A. odontolyticus, Bacteroides asaccharolyticus (B. gingivalis), B. melaninogenicus subspecies intermedius, and subspecies melaninogenicus, Capnocytophaga ochracea (B. ochraceus), and Fusobacterium nucleatum. Three B. melaninogenicus isolates showed plasmids of approximately 2.7-2.9 Mdalton (mega-dalton) molecular size. Restriction, enzyme digests of the plasmids demonstrated dissimilar patterns when electrophoresed on agarose gels. In other microorganisms, including the Actinomyces species, plasmids were not observed.

[1]  Y. Abiko,et al.  Characterization of the Streptococcus mutans plasmid pva318 cloned into Escherichia coli , 1981, Infection and immunity.

[2]  S. A. Syed.  Characteristics of Bacteroides asaccharolyticus from dental plaques of beagle dogs , 1980, Journal of clinical microbiology.

[3]  K. R. Jones,et al.  Transferable lincosamide-macrolide resistance in Bacteroides. , 1979, Plasmid.

[4]  J. van Houte,et al.  Role of Colonization in the Virulence of Actinomyces viscosus Strains T14-Vi and T14-Av , 1978, Infection and immunity.

[5]  S. Syed,et al.  Bacteriology of Human Experimental Gingivitis: Effect of Plaque and Gingivitis Score , 1978, Infection and immunity.

[6]  D. Guiney,et al.  Identification of a conjugative R plasmid in Bacteroides ochraceus capable of transfer to Escherichia coli , 1978, Nature.

[7]  R. H. Olsen,et al.  Isolation of large bacterial plasmids and characterization of the P2 incompatibility group plasmids pMG1 and pMG5 , 1978, Journal of bacteriology.

[8]  D J Ayers,et al.  A multiple plasmid-containing Escherichia coli strain: convenient source of size reference plasmid molecules. , 1978, Plasmid.

[9]  W. Loesche,et al.  New medium for isolation of Actinomyces viscosus and Actinomyces naeslundii from dental plaque , 1978, Journal of clinical microbiology.

[10]  F. L. Macrina,et al.  Evidence for a disseminated plasmid in Streptococcus mutans , 1978, Infection and immunity.

[11]  S. Socransky,et al.  Predominant cultivable microbiota in periodontosis. , 1977, Journal of periodontal research.

[12]  J. Slots.  The predominant cultivable microflora of advanced periodontitis. , 1977, Scandinavian journal of dental research.

[13]  J. A. Meyers,et al.  Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid , 1976, Journal of bacteriology.

[14]  F. L. Macrina,et al.  Extrachromosomal elements in a variety of strains representing the Bacteroides fragilis group of organisms , 1976, Infection and immunity.

[15]  K. Peindl,et al.  Antigens and Surface Components Associated with Virulence of Actinomyces viscosus , 1976, Journal of dental research.

[16]  S. Syed,et al.  Predominant cultivable flora isolated from human root surface caries plaque , 1975, Infection and immunity.

[17]  G. Bowden,et al.  Microbial variations in approximal dental plaque. , 1975, Caries research.

[18]  R. Keller,et al.  Isolation and characterization of several cryptic plasmids from clinical isolates of Bacteroides fragilis. , 1974, The Journal of infectious diseases.

[19]  S. Falkow,et al.  General Method for the Isolation of Plasmid Deoxyribonucleic Acid , 1973, Journal of bacteriology.

[20]  H. Jordan,et al.  Root surface caries: review of the literature and significance of the problem. , 1973, Journal of periodontology.

[21]  W. J. Loesche,et al.  The predominant cultivable flora of tooth surface plaque removed from institutionalized subjects. , 1972, Archives of oral biology.

[22]  J. Hardin,et al.  Chemotherapy of dental plaque infections. , 1970, Dental clinics of North America.