Tepidicaulis marinus gen. nov., sp. nov., a marine bacterium that reduces nitrate to nitrous oxide under strictly microaerobic conditions.

A moderately thermophilic, aerobic, stalked bacterium (strain MA2T) was isolated from marine sediments in Kagoshima Bay, Japan. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain MA2T was most closely related to the genera Rhodobium,Parvibaculum, and Rhodoligotrophos (92-93 % similarity) within the class Alphaproteobacteria. Strain MA2T was a Gram-stain-negative and stalked dimorphic bacteria. The temperature range for growth was 16-48 °C (optimum growth at 42 °C). This strain required yeast extract and NaCl (>1 %, w/v) for growth, tolerated up to 11 % (w/v) NaCl, and was capable of utilizing various carbon sources. The major cellular fatty acid and major respiratory quinone were C18 : 1ω7c and ubiquinone-10, respectively. The DNA G+C content was 60.7 mol%. Strain MA2T performed denitrification and produced N2O from nitrate under strictly microaerobic conditions. Strain MA2T possessed periplasmic nitrate reductase (Nap) genes but not membrane-bound nitrate reductase (Nar) genes. On the basis of this morphological, physiological, biochemical and genetic information a novel genus and species, Tepidicaulis marinus gen. nov., sp. nov., are proposed, with MA2T ( = NBRC 109643T = DSM 27167T) as the type strain of the species.

[1]  M. Hattori,et al.  Methylocaldum marinum sp. nov., a thermotolerant, methane-oxidizing bacterium isolated from marine sediments, and emended description of the genus Methylocaldum. , 2014, International journal of systematic and evolutionary microbiology.

[2]  M. Hattori,et al.  Methyloceanibacter caenitepidi gen. nov., sp. nov., a facultatively methylotrophic bacterium isolated from marine sediments near a hydrothermal vent. , 2014, International journal of systematic and evolutionary microbiology.

[3]  T. Ura,et al.  Shallow submarine hydrothermal activity with significant contribution of magmatic water producing talc chimneys in the Wakamiko Crater of Kagoshima Bay, southern Kyushu, Japan , 2013 .

[4]  C. Vetriani,et al.  Parvibaculum hydrocarboniclasticum sp. nov., a mesophilic, alkane-oxidizing alphaproteobacterium isolated from a deep-sea hydrothermal vent on the East Pacific Rise. , 2012, International journal of systematic and evolutionary microbiology.

[5]  H. Atomi,et al.  Rhodoligotrophos appendicifer gen. nov., sp. nov., an appendaged bacterium isolated from a freshwater Antarctic lake. , 2012, International journal of systematic and evolutionary microbiology.

[6]  Ying Liu,et al.  Characterization of a marine origin aerobic nitrifying-denitrifying bacterium. , 2012, Journal of bioscience and bioengineering.

[7]  M. Delgado,et al.  Denitrification in Sinorhizobium meliloti. , 2011, Biochemical Society transactions.

[8]  Y. Kamagata,et al.  A distinct freshwater-adapted subgroup of ANME-1 dominates active archaeal communities in terrestrial subsurfaces in Japan. , 2011, Environmental microbiology.

[9]  M. Nei,et al.  MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. , 2011, Molecular biology and evolution.

[10]  Liping Wang,et al.  Parvibaculum indicum sp. nov., isolated from deep-sea water. , 2011, International journal of systematic and evolutionary microbiology.

[11]  F. Rainey,et al.  Tepidamorphus gemmatus gen. nov., sp. nov., a slightly thermophilic member of the Alphaproteobacteria. , 2010, Systematic and applied microbiology.

[12]  A. Ravishankara,et al.  Nitrous Oxide (N2O): The Dominant Ozone-Depleting Substance Emitted in the 21st Century , 2009, Science.

[13]  David J Van Horn,et al.  Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities , 2009, Applied and Environmental Microbiology.

[14]  Kazuhiko Shimada,et al.  Marine shallow-water hydrothermal activity and mineralization at the Wakamiko crater in Kagoshima bay, south Kyushu, Japan , 2008 .

[15]  W. Ludwig,et al.  SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB , 2007, Nucleic acids research.

[16]  M. Höfle,et al.  Anderseniella baltica gen. nov., sp. nov., a novel marine bacterium of the Alphaproteobacteria isolated from sediment in the central Baltic Sea. , 2007, International journal of systematic and evolutionary microbiology.

[17]  J. Imhoff,et al.  Rhodobium gokarnense sp. nov., a novel phototrophic alphaproteobacterium from a saltern. , 2007, International journal of systematic and evolutionary microbiology.

[18]  D. B. Nedwell,et al.  Diversity and Abundance of Nitrate Reductase Genes (narG and napA), Nitrite Reductase Genes (nirS and nrfA), and Their Transcripts in Estuarine Sediments , 2007, Applied and Environmental Microbiology.

[19]  H. Uchiyama,et al.  Characterization of the Aerobic Denitrification in Mesorhizobium sp. Strain NH-14 in Comparison with that in Related Rhizobia , 2005 .

[20]  B. Tindall,et al.  Parvibaculum lavamentivorans gen. nov., sp. nov., a novel heterotroph that initiates catabolism of linear alkylbenzenesulfonate. , 2004, International journal of systematic and evolutionary microbiology.

[21]  Zhemin Zhou,et al.  Aerobic Denitrifying Bacteria That Produce Low Levels of Nitrous Oxide , 2003, Applied and Environmental Microbiology.

[22]  C. Nevison,et al.  Global distribution and production of N2O in the subsurface ocean , 2003 .

[23]  M. Kanemori,et al.  Nitrate reductase from the magnetotactic bacterium Magnetospirillum magnetotacticum MS-1: purification and sequence analyses. , 2003, Canadian journal of microbiology.

[24]  V. Stewart,et al.  Periplasmic Nitrate Reductase (NapABC Enzyme) Supports Anaerobic Respiration by Escherichia coli K-12 , 2002, Journal of bacteriology.

[25]  Y. Kamagata,et al.  Burkholderia kururiensis sp. nov., a trichloroethylene (TCE)-degrading bacterium isolated from an aquifer polluted with TCE. , 2000, International journal of systematic and evolutionary microbiology.

[26]  K. Timmis,et al.  An evaluation of terminal-restriction fragment length polymorphism (T-RFLP) analysis for the study of microbial community structure and dynamics. , 2000, Environmental microbiology.

[27]  F. Rainey,et al.  Thauera mechernichensis sp. nov., an aerobic denitrifier from a leachate treatment plant. , 1999, International journal of systematic bacteriology.

[28]  N. Bernet,et al.  Microvirgula aerodenitrificans gen. nov., sp. nov., a new gram-negative bacterium exhibiting co-respiration of oxygen and nitrogen oxides up to oxygen-saturated conditions. , 1998, International journal of systematic bacteriology.

[29]  L. Frette,et al.  Aerobic denitrifiers isolated from an alternating activated sludge system , 1997 .

[30]  D. Richardson,et al.  Effect of carbon substrate and aeration on nitrate reduction and expression of the periplasmic and membrane-bound nitrate reductases in carbon-limited continuous cultures of Paracoccus denitrificans Pd1222. , 1997, Microbiology.

[31]  J. G. Kuenen,et al.  Confirmation of 'aerobic denitrification' in batch cultures, using gas chromatography and 15N mass spectrometry , 1995 .

[32]  A. Hiraishi,et al.  A new genus of marine budding phototrophic bacteria, Rhodobium gen. nov., which includes Rhodobium orientis sp. nov. and Rhodobium marinum comb. nov. , 1995, International journal of systematic bacteriology.

[33]  Eiichi Mikami,et al.  Isolation and Characterization of a Novel Thermophilic Methanosaeta Strain , 1991 .

[34]  S. Goodison,et al.  16S ribosomal DNA amplification for phylogenetic study , 1991, Journal of bacteriology.

[35]  D. Richardson,et al.  Periplasmic and membrane‐bound respiratory nitrate reductases in Thiosphaera pantotropha , 1990, FEBS letters.

[36]  N. Saitou,et al.  The neighbor-joining method: a new method for reconstructing phylogenetic trees. , 1987, Molecular biology and evolution.

[37]  U. Rönner,et al.  Denitrification rates in the low-oxygen waters of the stratified baltic proper. , 1985, Applied and environmental microbiology.

[38]  R. Blakemore,et al.  Denitrification and Assimilatory Nitrate Reduction in Aquaspirillum magnetotacticum , 1983, Applied and environmental microbiology.

[39]  J. Kuenen,et al.  Thiosphaera pantotropha gen. nov. sp. nov., a Facultatively Anaerobic, Facultatively Autotrophic Sulphur Bacterium , 1983 .

[40]  F. Widdel,et al.  Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids , 1983, Archives of Microbiology.

[41]  F. Widdel,et al.  Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids , 1981, Archives of Microbiology.

[42]  R S Wolfe,et al.  Isolation and pure culture of a freshwater magnetic spirillum in chemically defined medium , 1979, Journal of bacteriology.

[43]  T. Yoshinari.  Nitrous oxide in the sea , 1976 .

[44]  Y. Sako,et al.  Distribution and diversity of anaerobic ammonium oxidation (anammox) bacteria in the sediment of a eutrophic freshwater lake, Lake Kitaura, Japan. , 2011, Microbes and environments.

[45]  Connor J. Liu,et al.  Isolation and Characterization of Novel , 2010 .

[46]  W. Salas,et al.  Methane and Nitrous Oxide Emissions from Natural Sources , 2010 .

[47]  Hiroyuki Kimura,et al.  Denitrification activity and relevant bacteria revealed by nitrite reductase gene fragments in soil of temperate mixed forest. , 2008, Microbes and environments.

[48]  J. Bowman.  The Methanotrophs — The Families Methylococcaceae and Methylocystaceae , 2006 .

[49]  J. Felsenstein.  Evolutionary trees from DNA sequences: A maximum likelihood approach , 2005, Journal of Molecular Evolution.

[50]  S. Takaichi,et al.  Roseiflexus castenholzii gen. nov., sp. nov., a thermophilic, filamentous, photosynthetic bacterium that lacks chlorosomes. , 2002, International journal of systematic and evolutionary microbiology.