Cholinergic innervation of the rat hippocampus as revealed by choline acetyltransferase immunocytochemistry: A combined light and electron microscopic study

The cholinergic innervation of the rat hippocampus proper and fascia dentata was investigated by using a monoclonal antibody against choline acetyltransferase (ChAT). At the light microscopic level, thin varicose ChAT‐immunoreactive fibers were observed mainly in the vicinity of the pyramidal and granular layers where they formed a fine network around proximal dendrites of pyramidal and granule cells. In addition, many ChAT‐immunoreactive fibers were found in the hilar region and in stratum oriens, radiatum, and lacunosum‐moleculare of all hippocampal sectors.

[1]  A. Siegel,et al.  Efferent connections of the septal area in the rat: An analysis utilizing retrograde and anterograde transport methods , 1977, Brain Research.

[2]  L. Sternberger,et al.  THE UNLABELED ANTIBODY ENZYME METHOD OF IMMUNOHISTOCHEMISTRY PREPARATION AND PROPERTIES OF SOLUBLE ANTIGEN-ANTIBODY COMPLEX (HORSERADISH PEROXIDASE-ANTIHORSERADISH PEROXIDASE) AND ITS USE IN IDENTIFICATION OF SPIROCHETES , 1970, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[3]  D. Amaral,et al.  The distribution of acetylcholinesterase in the hippocampal formation of the monkey , 1984, The Journal of comparative neurology.

[4]  D. Prince,et al.  Cholinergic excitation of mammalian hippocampal pyramidal cells , 1982, Brain Research.

[5]  C. Shute,et al.  Confirmation from choline acetylase analyses of a massive cholinergic innervation to the rat hippocampus , 1967, The Journal of physiology.

[6]  C. Ribak,et al.  Ultrastructure of the pyramidal basket cells in the dentate gyrus of the rat , 1980, The Journal of comparative neurology.

[7]  P. Mcgeer,et al.  Choline acetyltransferase: purification and immunohistochemical localization. , 1984, Life sciences.

[8]  G. Lynch,et al.  Anatomical and functional aspects of the septo-hippocampal projections. , 1977, Ciba Foundation symposium.

[9]  M. Frotscher.  Mossy fibres form synapses with identified pyramidal basket cells in the CA3 region of the guinea-pig hippocampus: a combined Golgi-electron microscope study , 1985, Journal of neurocytology.

[10]  H. Takagi,et al.  Topographic organization of the projection from the forebrain subcortical areas to the hippocampal formation of the rat , 1980, Neuroscience Letters.

[11]  G. Rose,et al.  Release and transfer of [3H]adenosine derivatives in the cholinergic septal system , 1977, Brain Research.

[12]  J. E. Vaughn,et al.  Immunocytochemical localization of choline acetyltransferase in rat cerebral cortex: A study of cholinergic neurons and synapses , 1985, The Journal of comparative neurology.

[13]  M. Woodruff,et al.  Cholinergic and non-cholinergic septo-hippocampal projections: a double-label horseradish peroxidase-acetylcholinesterase study in the rabbit , 1984, Brain Research.

[14]  H. Fibiger,et al.  The organization and some projections of cholinergic neurons of the mammalian forebrain , 1982, Brain Research Reviews.

[15]  M. Mesulam,et al.  Atlas of cholinergic neurons in the forebrain and upper brainstem of the macaque based on monoclonal choline acetyltransferase immunohistochemistry and acetylcholinesterase histochemistry , 1984, Neuroscience.

[16]  P. Somogyi,et al.  A note on the use of picric acid-paraformaldehyde-glutaraldehyde fixative for correlated light and electron microscopic immunocytochemistry , 1982, Neuroscience.

[17]  W. Cowan,et al.  Autoradiographic Studies of the Development and Connections of the Septal Area in the Rat , 1976 .

[18]  G. Lynch,et al.  The distribution of septal projections to the hippocampus of the rat , 1973, The Journal of comparative neurology.

[19]  R. Dingledine,et al.  The excitatory action of acetylcholine on hippocampal neurones of the guinea pig and rat maintained in vitro , 1981, Brain Research.

[20]  A. Alonso,et al.  A study of the reciprocal connections between the septum and the entorhinal area using anterograde and retrograde axonal transport methods in the rat brain , 1984, The Journal of comparative neurology.

[21]  J. Davis,et al.  A study of the rat septohippocampal pathway using anterograde transport of horseradish peroxidase , 1981, Neuroscience.

[22]  C. Shute,et al.  The cholinergic limbic system: projections to hippocampal formation, medial cortex, nuclei of the ascending cholinergic reticular system, and the subfornical organ and supra-optic crest. , 1967, Brain : a journal of neurology.

[23]  M. Frotscher,et al.  Commissural afferents innervate glutamate decarboxylase immunoreactive non-pyramidal neurons in the guinea pig hippocampus , 1984, Neuroscience Letters.

[24]  J. E. Vaughn,et al.  Organization and morphological characteristics of cholonergic neurons: an immunocytochemical study with a monoclonal antibody to choline acetyltransferase , 1983, Brain Research.

[25]  Cholinergic systems in mammalian brain identified with antibodies against choline acetyltransferase , 1984, Neurochemistry International.

[26]  T. Blackstad,et al.  CHOLINESTERASE IN THE HIPPOCAMPAL REGION , 1964 .

[27]  T. Freund,et al.  Cholinergic synapses in the rat brain: a correlated light and electron microscopic immunohistochemical study employing a monoclonal antibody against choline acetyltransferase , 1984, Brain Research.

[28]  J. P. Chandler,et al.  The septohippocampal projection in the rat: An electron microscopic horseradish peroxidase study , 1983, Neuroscience.

[29]  S. Hsu,et al.  Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. , 1981, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[30]  B. Srebro,et al.  Changes in acetylcholinesterase and distribution of degenerating fibres in the hippocampal region after septal lesions in the rat. , 1973, Brain research.

[31]  M. Frotscher,et al.  Commissural afferents to the rat hippocampus terminate on vasoactive intestinal polypeptide-like immunoreactive non-pyramidal neurons. An EM immunocytochemical degeneration study , 1983, Brain Research.

[32]  F. Fonnum.  TOPOGRAPHICAL AND SUBCELLULAR LOCALIZATION OF CHOLINE ACETYLTRANSFERASE IN RAT HIPPOCAMPAL REGION , 1970, Journal of neurochemistry.

[33]  M. Mesulam,et al.  Central cholinergic pathways in the rat: An overview based on an alternative nomenclature (Ch1–Ch6) , 1983, Neuroscience.

[34]  C. Ribak,et al.  Direct commissural connections to the basket cells of the hippocampal dentate gyrus: anatomical evidence for feed-forward inhibition , 1984, Journal of neurocytology.

[35]  M. Frotscher,et al.  Commissural fibers terminate on non-pyramidal neurons in the guinea pig hippocampus — a combined Golgi/EM degeneration study , 1983, Brain Research.

[36]  Roger A. Nicoll,et al.  The pharmacology of cholinergic excitatory responses in hippocampal pyramidal cells , 1984, Brain Research.

[37]  G. W. Hoesen,et al.  Enhanced acetylcholinesterase staining in the hippocampal perforant pathway zone after combined lesions of the septum and entorhinal cortex , 1983, Brain Research.

[38]  S. Landis,et al.  Afferents to the hippocampus of the rat studied with the method of retrograde transport of horseradish peroxidase. , 1974, Brain research.

[39]  J. Storm-Mathisen.  QUANTITATIVE HISTOCHEMISTRY OF ACETYL‐CHOLINESTERASE IN RAT HIPPOGAMPAL REGION CORRELATED TO HISTOCHEMICAL STAINING , 1970, Journal of neurochemistry.

[40]  H. Fibiger,et al.  Analysis of the septo-hippocampal pathway by light and electron microscopic autoradiography , 1976, Brain Research.

[41]  M. Frotscher,et al.  Lesion‐induced mossy fibers to the molecular layer of the rat fascia dentata: Identification of postsynaptic granule cells by the Golgi‐EM technique , 1983, The Journal of comparative neurology.

[42]  W. Cowan,et al.  The connections of the septal region in the rat , 1979, The Journal of comparative neurology.

[43]  P. Somogyi,et al.  The section-Golgi impregnation procedure. 1. Description of the method and its combination with histochemistry after intracellular iontophoresis or retrograde transport of horseradish peroxidase , 1983, Neuroscience.

[44]  G. Raisman.  The connexions of the septum. , 1966, Brain : a journal of neurology.

[45]  H. Thoenen,et al.  Production of specific antisera and monoclonal antibodies to choline acetyltransferase: characterization and use for identification of cholinergic neurons. , 1982, The EMBO journal.