Photodynamic action of methylene blue: repair and mutation in Escherichia coli.

The effects of the photodynamic action of methylene blue (MB-PDA) on strains of Escherichia coli were investigated to determine whether the dye could be used in photodynamic therapy (PDT). Using the method of alkaline sucrose gradient sedimentation, it was shown that in darkness MB induces a type of prelesion in DNA that transforms into single-strand breaks in alkaline conditions, provided that the dye is present during the processing of the gradient. This prelesion is completely reversible if the cells are washed immediately to remove the dye. However, after illumination with white light, the prelesions become "fixed" stable lesions, irreversible even after successive washings. The lethal damage induced by MB-PDA in E. coli can be repaired by the excision-repair system (about 30%) and by the recA-dependent repair system (about 70%). Polymerase I enzyme participates actively in the repair of the damage. MB-PDA is a weak mutagen and the induction of mutations by this treatment is restricted to high survival rates. Moreover, MB-PDA does not induce the SOS system (an inducible repair system dependent of the recA and loxA genes products), as measured by Weigle reactivation. However, it seems that this treatment can impair the repair systems in E. coli.

[1]  Mccaughan Js.  Photodynamic therapy of malignant tumors. , 1988 .

[2]  C. Wilson,et al.  Photodynamic therapy of malignant tumours. , 1972, Lancet.

[3]  B. Hass,et al.  Photodynamic effects of dyes on bacteria. I. Mutagenesis by acridine orange in the dark in excision- and recombination-deficient strains of Escherichia coli. , 1978, Mutation research.

[4]  Takashi Ito.  Photodynamic Agents as Tools for Cell Biology , 1983 .

[5]  J E Kaufman,et al.  Photoradiation therapy for the treatment of malignant tumors. , 1978, Cancer research.

[6]  R. Richart.  A clinical staining test for the in vivo delineation of dysplasia and carcinoma in situ. , 1963, American Journal of Obstetrics and Gynecology.

[7]  D. Macphee,et al.  Induction of base-pair substitution and frameshift mutations in wild-type and repair-deficient strains of Salmonella typhimurium by the photodynamic action of methylene blue. , 1975, Mutation research.

[8]  R. Tyrrell.  MUTATION INDUCTION BY AND MUTATIONAL INTERACTION BETWEEN MONOCHROMATIC WAVELENGTH RADIATIONS IN THE NEAR‐ULTRAVIOLET AND VISIBLE RANGES , 1980, Photochemistry and photobiology.

[9]  C. Wilson,et al.  Photochemotherapy of glioma cells by visible light and hematoporphyrin. , 1975, Cancer research.

[10]  D. Kessel,et al.  CHEMISTRY OF HEMATOPORPHYRIN‐DERIVED PHOTOSENSITIZERS , 1987, Photochemistry and photobiology.

[11]  R. Tyrrell,et al.  Photodynamic Therapy of Infections , 1982 .

[12]  F. Figge,et al.  Cancer Detection and Therapy. Affinity of Neoplastic, Embryonic, and Traumatized Tissues for Porphyrins and Metalloporphyrins.∗ , 1948, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[13]  D. Gordon,et al.  Visual identification of an insulinoma using methylene blue , 1974 .

[14]  H. Rosenkranz,et al.  A study of the photoinduced mutagenicity of methylene blue. , 1977, Mutation research.

[15]  M. Radman,et al.  Indirect ultraviolet-reactivation of phage lambda. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[16]  H. E. Kubitschek,et al.  Photodynamic effects of dyes on bacteria. II. Genetic effects of broad-spectrum visible light in the presence of acridine dyes and methylene blue in chemostat cultures of Escherichia coli. , 1979, Mutation research.

[17]  H. Jacob.  IN VIVO PRODUCTION OF DNA SINGLE‐STRAND BREAKS BY PHOTODYNAMIC ACTION , 1971, Photochemistry and photobiology.

[18]  M. Radman,et al.  Ultraviolet reactivation and ultraviolet mutagenesis of λ in different genetic systems , 1971 .

[19]  R. Williams,et al.  Reconstruction in vivo of Irradiated Escherichia coli Deoxyribonucleic Acid; the Rejoining of Broken Pieces , 1966, Nature.

[20]  K. Igarashi,et al.  In vivo staining test with methylene blue for bladder cancer. , 1983, The Journal of urology.

[21]  F. Figge,et al.  Fluorescence of human lymphatic and cancer tissues following high doses of intravenous hematoporphyrin , 1955, Surgical forum.

[22]  R. Webb.  Near-UV mutagenesis: photoreactivation of 365-nm-induced mutational lesions in Escherichia coli WP2s , 1978, Journal of bacteriology.