Digital image acquisition in in vivo confocal microscopy

A flexible system for the real‐time acquisition of in vivo images has been developed. Images are generated using a tandem scanning confocal microscope interfaced to a low‐light‐level camera. The video signal from the camera is digitized and stored using a Gould image processing system with a real‐time digital disk (RTDD). The RTDD can store up to 3200 512 times 512 pixel images at video rates (30 images s−1). Images can be input directly from the camera during the study, or off‐line from a Super VHS video recorder. Once a segment of experimental interest is digitized onto the RTDD, the user can interactively step through the images, average stable sequences, and identify candidates for further processing and analysis. Examples of how this system can be used to study the physiology of various organ systems in vivo are presented.

[1]  H D Cavanagh,et al.  Confocal microscopy of the living eye. , 1990, The CLAO journal : official publication of the Contact Lens Association of Ophthalmologists, Inc.

[2]  Alan Boyde,et al.  Tandem-scanning reflected-light microscope. , 1985 .

[3]  J Bryan,et al.  Validation of an imaging system: steps to evaluate and validate a microscope imaging system for quantitative studies. , 1989, Methods in cell biology.

[4]  A Boyde,et al.  In Vivo Microscopy Using the Tandem Scanning Microscope a , 1986, Annals of the New York Academy of Sciences.

[5]  A Boyde,et al.  Tandem‐Scanning (Confocal) Microscopy of the Full‐Thickness Cornea , 1985, Cornea.

[6]  H. D. Cavanagh,et al.  In vivo, real-time confocal imaging. , 1991, Journal of electron microscopy technique.

[7]  C. Sheppard,et al.  Theory and practice of scanning optical microscopy , 1984 .