Calcium carbonate nanoparticles: a host matrix for the construction of highly sensitive amperometric phenol biosensor.

We reported on the utilization of a novel attractive nanoscaled calcium carbonate (nano-CaCO(3))-polyphenol oxidase (PPO) biocomposite to create a highly responsive phenol biosensor. The phenol sensor could be easily achieved by casting the biocomposite on the surface of glassy carbon electrode (GCE) via the cross-linking step by glutaraldehyde. The special three-dimensional structure, porous morphology, hydrophilic and biocompatible properties of the nano-CaCO(3) matrix resulted in high enzyme loading, and the enzyme entrapped in this matrix retained its activity to a large extent. The proposed PPO/nano-CaCO(3) exhibited dramatically developed analytical performance such as such as a broad determination range (6 x 10(-9) -2 x 10(-5)M), a short response time (less than 12 s), high sensitivity (474 mA M(-1)), subnanomolar detection limit (0.44 nM at a signal to noise ratio of 3) and good long-term stability (70% remained after 56 days). In addition, effects of pH value, applied potential, temperature and electrode construction were investigated and discussed.

[1]  T. Yamane,et al.  Intensification of lipase performance in a transesterification reaction by immobilization on CaCO3 powder , 1998 .

[2]  N. Chen,et al.  Effect of nano-CaCO3 on mechanical properties of PVC and PVC/Blendex blend , 2004 .

[3]  Silvia Fabiano,et al.  Amperometric tyrosinase based biosensor using an electrogenerated polythiophene film as an entrapment support. , 2003, Talanta.

[4]  Guo-Li Shen,et al.  A mediator-free phenol biosensor based on immobilizing tyrosinase to ZnO nanoparticles. , 2006, Analytical biochemistry.

[5]  Aiguo Wu,et al.  A method to construct a third-generation horseradish peroxidase biosensor: self-assembling gold nanoparticles to three-dimensional sol-gel network. , 2002, Analytical chemistry.

[6]  S. Cosnier,et al.  Amperometric phenol biosensor based on laponite clay-chitosan nanocomposite matrix. , 2007, Biosensors & bioelectronics.

[7]  L. Gorton,et al.  Rate-limiting steps of tyrosinase-modified electrodes for the detection of catechol. , 1996, Analytical chemistry.

[8]  P Labbé,et al.  Polyphenol oxidase-catechol: an electroenzymatic model system for characterizing the performance of matrices for biosensors. , 1996, Talanta.

[9]  Jun‐Jie Zhu,et al.  Reagentless biosensor for phenolic compounds based on tyrosinase entrapped within gelatine film , 2005, Analytical and bioanalytical chemistry.

[10]  J P Hervás Pérez,et al.  Amperometric tyrosinase biosensor based on polyacrylamide microgels. , 2006, Biosensors & bioelectronics.

[11]  Xiaolong Xu,et al.  The FT-IR spectrometric analysis of the changes of polyphenol oxidase II secondary structure , 2003 .

[12]  S. Cosnier,et al.  Development of amperometric biosensor for glucose based on a novel attractive enzyme immobilization matrix: calcium carbonate nanoparticles. , 2007, Biosensors & bioelectronics.

[13]  S. Cosnier,et al.  A New Polyphenol Oxidase Biosensor Mediated by Azure B in Laponite Clay Matrix , 2003 .

[14]  Serge Cosnier,et al.  Direct electrochemistry and electrocatalysis of hemoglobin entrapped in composite matrix based on chitosan and CaCO3 nanoparticles , 2007 .

[15]  L. Simpson.  Electrochemically generated CaCO3 deposits on iron studied with FTIR and Raman spectroscopy , 1998 .

[16]  S. Cosnier,et al.  Development of a PPO-poly(amphiphilic pyrrole) electrode for on site monitoring of phenol in aqueous effluents , 1999 .

[17]  G. Sukhorukov,et al.  Protein encapsulation via porous CaCO3 microparticles templating. , 2004, Biomacromolecules.

[18]  Reinhard Renneberg,et al.  Encapsulation of glucose oxidase microparticles within a nanoscale layer-by-layer film: immobilization and biosensor applications. , 2003, Biosensors & bioelectronics.

[19]  S. Cosnier,et al.  Layered double hydroxides: an attractive material for electrochemical biosensor design. , 2003, Analytical chemistry.

[20]  S. Dong,et al.  Silica sol-gel composite film as an encapsulation matrix for the construction of an amperometric tyrosinase-based biosensor. , 2000, Biosensors & bioelectronics.

[21]  Guo-Li Shen,et al.  A Mediator-Free Tyrosinase Biosensor Based on ZnO Sol-Gel Matrix , 2005 .

[22]  H. Duckworth,et al.  Physicochemical and kinetic properties of mushroom tyrosinase. , 1970, The Journal of biological chemistry.

[23]  M. L. Mena,et al.  Development of a tyrosinase biosensor based on gold nanoparticles-modified glassy carbon electrodes: Application to the measurement of a bioelectrochemical polyphenols index in wines , 2005 .

[24]  J M Pingarrón,et al.  Development of a high analytical performance-tyrosinase biosensor based on a composite graphite-Teflon electrode modified with gold nanoparticles. , 2006, Biosensors & bioelectronics.

[25]  Guo-Li Shen,et al.  A phenol biosensor based on immobilizing tyrosinase to modified core-shell magnetic nanoparticles supported at a carbon paste electrode , 2005 .

[26]  I. Popescu,et al.  Phenols monitoring and Hill coefficient evaluation using tyrosinase-based amperometric biosensors. , 2004, Bioelectrochemistry.

[27]  J. Kong,et al.  A sensitive mediator-free tyrosinase biosensor based on an inorganic-organic hybrid titania sol-gel matrix , 2003 .

[28]  Huangxian Ju,et al.  Mediator-free phenol sensor based on titania sol-gel encapsulation matrix for immobilization of tyrosinase by a vapor deposition method. , 2003, Biosensors & bioelectronics.

[29]  J. Kong,et al.  Probing trace phenols based on mediator-free alumina sol--gel-derived tyrosinase biosensor. , 2000, Analytical chemistry.

[30]  S. Poyard,et al.  Optimization of an inorganic/bio-organic matrix for the development of new glucose biosensor membranes , 1998 .

[31]  Qin Xu,et al.  Porous Gold-Nanoparticle−CaCO3 Hybrid Material: Preparation, Characterization, and Application for Horseradish Peroxidase Assembly and Direct Electrochemistry , 2006 .

[32]  David J. Best,et al.  THE DETERMINATION OF P-CRESOL IN CHLOROFORM WITH AN ENZYME ELECTRODE USED IN THE ORGANIC-PHASE , 1988 .

[33]  S. Cosnier,et al.  Hybrid material based on chitosan and layered double hydroxides: characterization and application to the design of amperometric phenol biosensor. , 2007, Biomacromolecules.

[34]  Huaiguo Xue,et al.  A highly stable biosensor for phenols prepared by immobilizing polyphenol oxidase into polyaniline-polyacrylonitrile composite matrix. , 2002, Talanta.

[35]  J. Heinze,et al.  Optical biochemical sensor for determining hydroperoxides in nonpolar organic liquids as archetype for sensors consisting of amphiphilic conetworks as immobilisation matrices , 2006, Analytical and bioanalytical chemistry.

[36]  C. Tillyer,et al.  The development of a catechol enzyme electrode and its possible use for the diagnosis and monitoring of neural crest tumours. , 1991, Biosensors & bioelectronics.