Impact of thermal processing on ELISA detection of peanut allergens.

This study examined the effect of heat treatment on the solubility of peanut proteins and compared the performances of two commercial ELISA kits (Veratox Quantitative Peanut Allergen Test and BioKits Peanut Assay Kit) for quantitation of peanut residues as affected by different heat treatments (moist and dry heat) and detection targets (mixture of proteins vs specific protein). Both laboratory-prepared and commercial peanut flour preparations were used for the evaluation. The two ELISA kits tended to underestimate the levels of protein in samples that were subjected to elevated heat, respectively, by more than 60- or 400-fold lower for the autoclaved samples and by as much as 70- or 2000-fold lower for the dark-roast commercial flour samples. The BioKits test, which employs antibodies specific to a heat labile protein (Ara h 1), in general exhibited a greater degree of underestimation. These results suggest that commercial ELISA kits may not be able to accurately determine the amount of proteins present in thermally processed foods due to changes in the solubility and immunoreactivity of the target proteins. Users need to be aware of such limitations before applying ELISA kits for evaluation of food allergen control programs.

[1]  K. Banasiak.  Here Today, Gone Tomorrow , 2005 .

[2]  K. Roux,et al.  Effects of processing on immunoreactivity of cashew nut (Anacardium occidentale L.) seed flour proteins. , 2008, Journal of agricultural and food chemistry.

[3]  A. Pomés,et al.  Quantification of Ara h 1 in peanuts: why roasting makes a difference , 2006, Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology.

[4]  J. Wal.  Thermal processing and allergenicity of foods , 2003, Allergy.

[5]  William Ross,et al.  Validation procedures for quantitative food allergen ELISA methods: community guidance and best practices. , 2010, Journal of AOAC International.

[6]  Douglas L. Park,et al.  Performance Tested Method multiple laboratory validation study of ELISA-based assays for the detection of peanuts in food. , 2005, Journal of AOAC International.

[7]  W. Burks,et al.  A COMPARISON OF COMMERCIALLY AVAILABLE PEANUT ELISA TEST KITS ON THE ANALYSIS OF SAMPLES OF DARK AND MILK CHOCOLATE , 2002, Journal of immunoassay & immunochemistry.

[8]  P. Davis,et al.  How can thermal processing modify the antigenicity of proteins? , 2001, Allergy.

[9]  Anne Muñoz-Furlong,et al.  Further fatalities caused by anaphylactic reactions to food, 2001-2006. , 2007, The Journal of allergy and clinical immunology.

[10]  A. Pomés,et al.  Peanut Allergen (Ara h 1) Detection in Foods Containing Chocolate , 2004 .

[11]  K. E. Løvberg,et al.  Extractability, stability, and allergenicity of egg white proteins in differently heat-processed foods. , 2007, Journal of AOAC International.

[12]  T. Whitaker,et al.  Effect of processing on recovery and variability associated with immunochemical analytical methods for multiple allergens in a single matrix: sugar cookies. , 2012, Journal of agricultural and food chemistry.

[13]  E. Anklam,et al.  Effect of roasting history and buffer composition on peanut protein extraction efficiency. , 2004, Molecular nutrition & food research.

[14]  Jonathan W DeVries,et al.  Cleaning and other control and validation strategies to prevent allergen cross-contact in food-processing operations. , 2008, Journal of food protection.

[15]  Patricia Schubert-Ullrich,et al.  Commercialized rapid immunoanalytical tests for determination of allergenic food proteins: an overview , 2009, Analytical and bioanalytical chemistry.

[16]  Richard B Raybourne,et al.  Evaluation of extraction buffers using the current approach of detecting multiple allergenic and nonallergenic proteins in food. , 2004, Journal of AOAC International.

[17]  Sarah F. Davis,et al.  Analysis and evaluation of food manufacturing practices used to address allergen concerns , 2006 .

[18]  Brittany L. White,et al.  Compositional and mechanical properties of peanuts roasted to equivalent colors using different time/temperature combinations. , 2012, Journal of food science.

[19]  J. Wal,et al.  Influence of thermal processing on the allergenicity of peanut proteins. , 2005, Journal of agricultural and food chemistry.

[20]  S. Maleki,et al.  Processing can alter the properties of peanut extract preparations. , 2010, Journal of agricultural and food chemistry.

[21]  H. Sampson,et al.  Fatalities due to anaphylactic reactions to foods. , 2001, The Journal of allergy and clinical immunology.

[22]  H. D. de Jongh,et al.  Heat-induced Conformational Changes of Ara h 1, a Major Peanut Allergen, Do Not Affect Its Allergenic Properties* , 1999, The Journal of Biological Chemistry.

[23]  Cesar M. Compadre,et al.  Structure of the Major Peanut Allergen Ara h 1 May Protect IgE-Binding Epitopes from Degradation1 , 2000, The Journal of Immunology.

[24]  Steve L Taylor,et al.  Allergen immunoassays—considerations for use of naturally incurred standards , 2009, Analytical and bioanalytical chemistry.

[25]  Katie Banaszewski,et al.  Effect of heat treatment on the quantitative detection of egg protein residues by commercial enzyme-linked immunosorbent assay test kits. , 2010, Journal of agricultural and food chemistry.

[26]  Melanie L Downs,et al.  Effects of thermal processing on the enzyme-linked immunosorbent assay (ELISA) detection of milk residues in a model food matrix. , 2010, Journal of agricultural and food chemistry.

[27]  N. Rigby,et al.  Boiling peanut Ara h 1 results in the formation of aggregates with reduced allergenicity. , 2011, Molecular nutrition & food research.

[28]  Motohiro Ebisawa,et al.  Japan food allergen labeling regulation--history and evaluation. , 2011, Advances in food and nutrition research.

[29]  E. Anklam,et al.  Inter-laboratory validation study of five commercial ELISA test kits for the determination of peanut proteins in biscuits and dark chocolate , 2005, Food additives and contaminants.

[30]  B. Wüthrich,et al.  Comparison of commercially available ELISA kits with human sera-based detection methods for peanut allergens in foods , 2003, Food additives and contaminants.