Detection of Aflatoxin from Some Aspergillus sp. Isolated from Wheat Seeds

Ten seed borne fungi (Alternaria sp., Aspergillus sp., Aureobasidium sp., Cladosporium sp., Dreschslera sp., Penicillium sp., Rhizoctonia sp., Stemphylium sp., Mucor sp. and Rhizopus sp.) were isolated and identified from two wheat varieties, the highest frequency of seed borne fungi was observed on wheat cultivar site Mol14 Alternaria sp.. Their mean and standard deviation was (5.5 ± 1.69) while the lowest frequency fungal isolated was Dreschslera sp. and Rhizopus sp.. Their mean and standard deviation was (0.1 ± 0.64). The aflatoxin-producing isolates appeared as gray or black colonies in the UV photographs, whereas nonproducing isolates appeared as white colonies, the plate five colony four (P5CO4) showed the positive results which means the presence of aflatoxin as compaired to the control which showed the ngative results. Ammonium Hydroxide Vapor-Induced Color Change method used which the dish was inverted and 1 or 2 drops of concentrated ammonium hydroxide solution are placed on the inside of the lid. The undersides of aflatoxin-producing colonies quickly turn plum-red after the bottom of the Petri dish has been inverted over the lid containing the ammonium hydroxide aspositive result in (P5CO4) and (P7CO4) observed. Essentially no color change occurs on the undersides of colonies that are not producing aflatoxinsthis indicate to the negative results (control). The main objective of this study is to isolation, identification and rapid detection of aflatoxin from wheat seed borne fungi.

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