Second harmonic generation χ tensor microscopy for tissue imaging

Important biological molecules such as collagen and myosin are strong second harmonic generators. The polarization dependence of second-harmonic generation(SHG) is a property of the second order susceptibility elements ( χ tensor) of the molecules that produce the signal. In this work, we used polarizationSHGmicroscopy to image and map pixel scale polarization anisotropy information of collagen-muscle junction of chicken wing and the dermis of human skin. We show that the variation in the χ tensor components can be displayed as images to show the contrast between different molecular sources of SHG.

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