Nanotechnology in Dental Sciences: Moving towards a Finer Way of Doing Dentistry

Nanotechnologies are predicted to revolutionize: (a) the control over materials properties at ultrafine scales; and (b) the sensitivity of tools and devices applied in various scientific and technological fields. In this short review, we argue that dentistry will be no exception to this trend. Here, we present a dynamic view of dental tissues, an adoption of which may lead to finer, more effective and minimally invasive reparation approaches. By doing so, we aim at providing insights into some of the breakthroughs relevant to understanding the genesis of dental tissues at the nanostructural level or generating dental materials with nanoscale critical boundaries. The lineage of the progress of dental science, including the projected path along the presumed nanotechnological direction of research and clinical application is mentioned too. We conclude by claiming that dentistry should follow the trend of probing matter at nanoscale that currently dominates both materials and biological sciences in order to improve on the research strategies and clinical techniques that have traditionally rested on mechanistic assumptions.

[1]  R. D'Souza,et al.  Dentin Matrix Protein 1 (DMP1): New and Important Roles for Biomineralization and Phosphate Homeostasis , 2007, Journal of dental research.

[2]  A Leith,et al.  Mineral and organic matrix interaction in normally calcifying tendon visualized in three dimensions by high-voltage electron microscopic tomography and graphic image reconstruction. , 1993, Journal of structural biology.

[3]  P. Fratzl,et al.  Mineralized collagen fibrils: a mechanical model with a staggered arrangement of mineral particles. , 2000, Biophysical journal.

[4]  G W Marshall,et al.  Mechanical properties of human dental enamel on the nanometre scale. , 2001, Archives of oral biology.

[5]  A. Daar,et al.  ‘Mind the gap’: science and ethics in nanotechnology , 2003, The Ethics of Nanotechnology, Geoengineering and Clean Energy.

[6]  Anthony M Iacopino,et al.  The influence of "new science" on dental education: current concepts, trends, and models for the future. , 2007, Journal of dental education.

[7]  Mingyun Li,et al.  Bacterial interactions in dental biofilm , 2011, Virulence.

[8]  H. Mattoussi,et al.  Investigating Biological Processes at the Single Molecule Level Using Luminescent Quantum Dots , 2009, Annals of Biomedical Engineering.

[9]  V. Uskoković,et al.  Zeta-potential and Particle Size Analysis of Human Amelogenins , 2010, Journal of dental research.

[10]  R. Service,et al.  Nanomaterials Show Signs of Toxicity , 2003, Science.

[11]  G. Vougiouklakis,et al.  Effect of a CPP-ACP agent on the demineralization and remineralization of dentine in vitro. , 2007, Journal of dentistry.

[12]  J. Moradian-Oldak,et al.  Dynamic light scattering study of an amelogenin gel-like matrix in vitro. , 2006, European journal of oral sciences.

[13]  J. Kirkham,et al.  Physico-chemical properties of crystal surfaces in matrix–mineral interactions during mammalian biomineralisation , 2002 .

[14]  S. Doak,et al.  NanoGenotoxicology: the DNA damaging potential of engineered nanomaterials. , 2009, Biomaterials.

[15]  P. Hansma,et al.  Molecular energy dissipation in nanoscale networks of dentin matrix protein 1 is strongly dependent on ion valence , 2008, Nanotechnology.

[16]  E. Beniash,et al.  Role of Macromolecular Assembly of Enamel Matrix Proteins in Enamel Formation , 2006, Journal of dental research.

[17]  D. Pashley,et al.  Comparative SEM and TEM observations of nanoleakage within the hybrid layer. , 1995, Operative dentistry.

[18]  J. Davies,et al.  The effect of discrete calcium phosphate nanocrystals on bone-bonding to titanium surfaces. , 2007, Biomaterials.

[19]  Vuk Uskokovi Nanomaterials and Nanotechnologies: Approaching the Crest of this Big Wave , 2008 .

[20]  S. Weiner,et al.  Bone structure: from ångstroms to microns , 1992, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[21]  J. Kinney,et al.  Biomechanical Perspective on the Remineralization of Dentin , 2009, Caries Research.

[22]  R. Naik,et al.  Rapid, Room‐Temperature Formation of Crystalline Calcium Molybdate Phosphor Microparticles via Peptide‐Induced Precipitation , 2006 .

[23]  Mark Hewko,et al.  Shedding new light on early caries detection. , 2008, Journal.

[24]  A. Cooper Chapter 6 Thermodynamics of protein folding and stability , 1999 .

[25]  Robert N Grass,et al.  Oxide nanoparticle uptake in human lung fibroblasts: effects of particle size, agglomeration, and diffusion at low concentrations. , 2005, Environmental science & technology.

[26]  D. Wong,et al.  Saliva: an emerging biofluid for early detection of diseases. , 2009, American journal of dentistry.

[27]  E. Wachtel,et al.  The structure of mineralized collagen fibrils. , 1989, Connective tissue research.

[28]  N. Nakabayashi,et al.  The promotion of adhesion by the infiltration of monomers into tooth substrates. , 1982, Journal of biomedical materials research.

[29]  G W Marshall,et al.  Mechanical properties of mineralized collagen fibrils as influenced by demineralization. , 2008, Journal of structural biology.

[30]  J T Czernuszka,et al.  Collagen-hydroxyapatite composites for hard tissue repair. , 2006, European cells & materials.

[31]  J. Davies,et al.  Bone bonding at natural and biomaterial surfaces. , 2007, Biomaterials.

[32]  E. Sachlos,et al.  Making tissue engineering scaffolds work. Review: the application of solid freeform fabrication technology to the production of tissue engineering scaffolds. , 2003, European cells & materials.

[33]  A. Díez-Pérez,et al.  The tissue diagnostic instrument. , 2009, The Review of scientific instruments.

[34]  Vuk Uskokovi Theoretical and Practical Aspects of Colloid Science and Self-Assembly Phenomena Revisited , 2008 .

[35]  Morton B. Brown,et al.  The bone diagnostic instrument II: indentation distance increase. , 2008, The Review of scientific instruments.

[36]  W. Edgar,et al.  Role of Saliva in Caries Models , 1995, Advances in dental research.

[37]  Limin Sun,et al.  Strength and fluoride release characteristics of a calcium fluoride based dental nanocomposite. , 2008, Biomaterials.

[38]  Mauro Ferrari,et al.  Nanomedicine--challenge and perspectives. , 2009, Angewandte Chemie.

[39]  G. Dibdin The water in human dental enamel and its diffusional exchange measured by clearance of tritiated water from enamel slabs of varying thickness. , 1993, Caries research.

[40]  Daniel Fried,et al.  Early caries imaging and monitoring with near-infrared light. , 2005, Dental clinics of North America.

[41]  A. Cooper Thermodynamics of Protein Folding and Stability , 1999 .

[42]  A. Alivisatos,et al.  Observation of Single Colloidal Platinum Nanocrystal Growth Trajectories , 2009, Science.

[43]  A Kishen,et al.  Hydromechanics in dentine: role of dentinal tubules and hydrostatic pressure on mechanical stress-strain distribution. , 2007, Dental materials : official publication of the Academy of Dental Materials.

[44]  Y. Vohra,et al.  Control of phase composition in hydroxyapatite/tetracalcium phosphate biphasic thin coatings for biomedical applications , 2005, Journal of materials science. Materials in medicine.

[45]  M. Tirrell,et al.  Self-Assembly in Materials Synthesis , 2005 .

[46]  J De Munck,et al.  In vitro effect of nanoleakage expression on resin-dentin bond strengths analyzed by microtensile bond test, SEM/EDX and TEM. , 2004, Biomaterials.

[47]  Philippe K Zysset,et al.  An application of nanoindentation technique to measure bone tissue Lamellae properties. , 2005, Journal of biomechanical engineering.

[48]  A. Boyde,et al.  Hydration effects on the micro-mechanical properties of bone , 2006 .

[49]  C. Bertolami The role and importance of research and scholarship in dental education and practice. , 2002, Journal of dental education.

[50]  In-Seop Lee,et al.  The effects of ion beam-assisted deposition of hydroxyapatite on the grit-blasted surface of endosseous implants in rabbit tibiae. , 2005, The International journal of oral & maxillofacial implants.

[51]  W. Dubinsky,et al.  Salivary biomarkers for the detection of malignant tumors that are remote from the oral cavity. , 2009, Clinics in laboratory medicine.

[52]  A. George,et al.  Biological Assemblies Provide Novel Templates for the Synthesis of Biocomposites and Facilitate Cell Adhesion , 2008 .

[53]  Seth Pettie,et al.  Mind the gap , 2006, Nature Reviews Drug Discovery.

[54]  Daniel Fried,et al.  Near-infrared imaging of developmental defects in dental enamel. , 2008, Journal of biomedical optics.

[55]  J. Moradian-Oldak,et al.  3. Protein-protein interactions of the developing enamel matrix. , 2006, Current topics in developmental biology.

[56]  H. Sano Microtensile Testing, Nanoleakage, and Biodegradation of Resin-Dentin Bonds , 2006, Journal of dental research.

[57]  L. Gower Biomimetic model systems for investigating the amorphous precursor pathway and its role in biomineralization. , 2008, Chemical reviews.

[58]  C. Smith,et al.  Cellular and chemical events during enamel maturation. , 1998, Critical reviews in oral biology and medicine : an official publication of the American Association of Oral Biologists.

[59]  Chunlin Qin,et al.  Dentin Extracellular Matrix (ECM) Proteins: Comparison to Bone ECM and Contribution to Dynamics of Dentinogenesis , 2003, Connective tissue research.

[60]  Vuk Uskokovi On Science of Metaphors and the Nature of Systemic Reasoning , 2009 .

[61]  E. Veerman,et al.  Oral Diseases , 2007, Annals of the New York Academy of Sciences.

[62]  P K Hansma,et al.  Sacrificial bonds in the interfibrillar matrix of bone. , 2005, Journal of musculoskeletal & neuronal interactions.

[63]  Scott A Saunders,et al.  Current practicality of nanotechnology in dentistry. Part 1: Focus on nanocomposite restoratives and biomimetics , 2009 .

[64]  V. Uskoković,et al.  Enzymatic processing of amelogenin during continuous crystallization of apatite , 2008, Journal of materials research.

[65]  Vuk Uskoković,et al.  Nanotechnologies: What we do not know , 2007 .

[66]  R O Ritchie,et al.  Ultrastructural examination of dentin using focused ion-beam cross-sectioning and transmission electron microscopy. , 2005, Micron.

[67]  Petra Gruber,et al.  A gaze into the crystal ball: Biomimetics in the year 2059 , 2009 .

[68]  Igor L. Medintz,et al.  Quantum dots: a powerful tool for understanding the intricacies of nanoparticle-mediated drug delivery , 2009, Expert opinion on drug delivery.

[69]  J. Ong,et al.  Evaluation of titanium plasma-sprayed and plasma-sprayed hydroxyapatite implants in vivo. , 2004, Biomaterials.

[70]  A. Boyde Transmission electron microscopy of ion beam thinned dentine , 2004, Cell and Tissue Research.

[71]  J. Kinney,et al.  Intrafibrillar Mineral May be Absent in Dentinogenesis Imperfecta Type II (DI-II) , 2001, Journal of dental research.

[72]  L. Wong,et al.  Oral Biofilms: Emerging Concepts in Microbial Ecology , 2010, Journal of dental research.

[73]  P. Marsh,et al.  The oral microflora--friend or foe? Can we decide? , 2006, International dental journal.

[74]  Shen Hu,et al.  Human saliva proteome analysis and disease biomarker discovery , 2007, Expert review of proteomics.

[75]  S. Habelitz,et al.  The cooperative self-assembly of 25 and 23kDa amelogenins. , 2008, Journal of structural biology.

[76]  M. Goswami,et al.  Aging Affects Two Modes of Nanoleakage Expression in Bonded Dentin , 2012 .

[77]  R. Naik,et al.  Identification of peptides that promote the rapid precipitation of germania nanoparticle networks via use of a peptide display library. , 2004, Chemical communications.

[78]  J. Granjeiro,et al.  Basic research methods and current trends of dental implant surfaces. , 2009, Journal of biomedical materials research. Part B, Applied biomaterials.

[79]  S Lees,et al.  Considerations regarding the structure of the mammalian mineralized osteoid from viewpoint of the generalized packing model. , 1987, Connective tissue research.

[80]  S. Stupp,et al.  Molecular manipulation of microstructures: biomaterials, ceramics, and semiconductors. , 1997, Science.

[81]  A. Farghaly,et al.  Probing nano-scale adhesion force between AFM and acid demineralized intertubular dentin: Moist versus dry dentin. , 2009, Journal of dentistry.

[82]  D. Depaola The revitalization of U.S. dental education. , 2008, Journal of dental education.

[83]  S. Marshall,et al.  The tooth attachment mechanism defined by structure, chemical composition and mechanical properties of collagen fibers in the periodontium. , 2007, Biomaterials.

[84]  J. Jansen,et al.  A mechanical evaluation of TiO2-gritblasted and Ca-P magnetron sputter coated implants placed into the trabecular bone of the goat: Part 1. , 2000, Clinical oral implants research.

[85]  B. Ferguson WHAT WE DO NOT KNOW. , 1916, California state journal of medicine.

[86]  M. Swain,et al.  Understanding the mechanical behaviour of human enamel from its structural and compositional characteristics. , 2008, Journal of the mechanical behavior of biomedical materials.

[87]  J. Moradian-Oldak,et al.  Amelogenins: assembly, processing and control of crystal morphology. , 2001, Matrix biology : journal of the International Society for Matrix Biology.

[88]  E. P. Katz,et al.  Structure and function of bone collagen fibrils. , 1973, Journal of molecular biology.

[89]  E. Beniash,et al.  Bio-inspired Synthesis of Mineralized Collagen Fibrils. , 2008, Crystal growth & design.

[90]  Paolo Vecchia,et al.  International statements and definitions of the precautionary principle , 2002, IEEE Technol. Soc. Mag..

[91]  W. Lacefield Current status of ceramic coatings for dental implants. , 1998, Implant dentistry.

[92]  G W Marshall,et al.  The dentin substrate: structure and properties related to bonding. , 1997, Journal of dentistry.

[93]  Jacqueline A. Cutroni,et al.  Sacrificial bonds and hidden length dissipate energy as mineralized fibrils separate during bone fracture , 2005, Nature materials.

[94]  Glenn Harlan Reynolds,et al.  Environmental Regulation of Nanotechnology : Some Preliminary Observations , 2001 .

[95]  Vuk Uskoković,et al.  On the Light Doves and Learning on Mistakes , 2009 .

[96]  K. Hojo,et al.  Bacterial Interactions in Dental Biofilm Development , 2009, Journal of dental research.

[97]  S. Caputi,et al.  Histologic and ultrastructural analysis of regenerated bone in maxillary sinus augmentation using a porcine bone-derived biomaterial. , 2006, Journal of periodontology.

[98]  M. Snead,et al.  Biological Organization of Hydroxyapatite Crystallites into a Fibrous Continuum Toughens and Controls Anisotropy in Human Enamel , 2001, Journal of dental research.

[99]  E. Beniash,et al.  pH triggered self-assembly of native and recombinant amelogenins under physiological pH and temperature in vitro. , 2007, Journal of structural biology.

[100]  J. Jansen,et al.  A mechanical evaluation of TiO2-gritblasted and Ca-P magnetron sputter coated implants placed into the trabecular bone of the goat: Part 1. , 2000, Clinical oral implants research.

[101]  Yunzhi Yang,et al.  A review on calcium phosphate coatings produced using a sputtering process--an alternative to plasma spraying. , 2005, Biomaterials.

[102]  Michael G. Sowa,et al.  Characterization of early dental caries by polarized Raman spectroscopy , 2006, SPIE BiOS.

[103]  Lyndon F Cooper,et al.  Advancing dental implant surface technology--from micron- to nanotopography. , 2008, Biomaterials.

[104]  S. Mann Biomineralization: Principles and Concepts in Bioinorganic Materials Chemistry , 2002 .

[105]  W. Landis,et al.  Mineral characterization in calcifying tissues: atomic, molecular and macromolecular perspectives. , 1996, Connective tissue research.

[106]  J. Jansen,et al.  A histological evaluation of TiO2-gritblasted and Ca-P magnetron sputter coated implants placed into the trabecular bone of the goat: Part 2. , 2000, Clinical oral implants research.

[107]  J. Kinney,et al.  The Importance of Intrafibrillar Mineralization of Collagen on the Mechanical Properties of Dentin , 2003, Journal of dental research.

[108]  W. G. Matthews,et al.  Nanoleakage: leakage within the hybrid layer. , 1995, Operative dentistry.