Bacterial biofilm disruption using laser-generated shockwaves

Bacterial related infections are a burden on the healthcare industry. A system was built to test the efficacy of laser generated shockwaves on S. epidermidis biofilms (RP62A) grown on polystyrene surfaces. The system is based on a Qswitched, ND:YAG pulsed laser with an output wavelength of 1.064 μm that ablates titanium-coated soda-lime glass. Results show that the system is capable of generating stress profiles that can effectively delaminate biofilm structures from polymer surfaces.

[1]  V. Gupta,et al.  Measurement of interface strength by a laser spallation technique , 1992 .

[2]  L A Crum,et al.  The significance of membrane changes in the safe and effective use of therapeutic and diagnostic ultrasound. , 1989, Physics in medicine and biology.

[3]  J M Dodick,et al.  Experimental studies on the development and propagation of shock waves created by the interaction of short Nd:YAG laser pulses with a titanium target: Possible implications for Nd:YAG laser phacolysis of the cataractous human lens , 1991, Journal of cataract and refractive surgery.

[4]  W Schaden,et al.  Extracorporeal Shock Wave Therapy of Nonunion or Delayed Osseous Union , 2001, Clinical orthopaedics and related research.

[5]  Paul Stoodley,et al.  Laser Disruption of Biofilm , 2008, The Laryngoscope.

[6]  Hideki Aita,et al.  Glycosaminoglycan degradation reduces mineralized tissue-titanium interfacial strength. , 2006, Journal of biomedical materials research. Part A.

[7]  G. Reid,et al.  Microbial Biofilms: Their Development and Significance for Medical Device—Related Infections , 1999, Journal of clinical pharmacology.

[8]  J. Yuan,et al.  Measurement of interface strength by the modified laser spallation technique. I - Experiment and simulation of the spallation process. II - Applications to metal/ceramic interfaces , 1993 .

[9]  V. Gupta,et al.  Measurement of the tensile strength of cell-biomaterial interface using the laser spallation technique. , 2008, Acta biomaterialia.

[10]  G. Grabner,et al.  Dodick laser phacolysis: thermal effects. , 1999, Journal of cataract and refractive surgery.

[11]  T J Flotte,et al.  Biological effects of laser-induced shock waves: structural and functional cell damage in vitro. , 1993, Ultrasound in medicine & biology.

[12]  Hiroshi Yoshida,et al.  Glass-modified stress waves for adhesion measurement of ultra thin films for device applications , 2003 .

[13]  Jun Yuan,et al.  Measurement of interface strength by the modified laser spallation technique. III. Experimental optimization of the stress pulse , 1993 .

[14]  Jun Yuan,et al.  The effect of microstructure and chemistry on the tensile strength of Nb/sapphire interfaces with and without interlayers of Sb and Cr , 1995 .

[15]  P Stoodley,et al.  Effect of low-intensity ultrasound upon biofilm structure from confocal scanning laser microscopy observation. , 1996, Biomaterials.

[16]  Thomas Bjarnsholt,et al.  Quorum-sensing blockade as a strategy for enhancing host defences against bacterial pathogens , 2007, Philosophical Transactions of the Royal Society B: Biological Sciences.

[17]  V. Gupta,et al.  Measurement of interface strength by the modified laser spallation technique. II. Applications to metal/ceramic interfaces , 1993 .

[18]  V. Gupta,et al.  Interferometry on diffuse surfaces in high‐velocity measurements , 1993 .

[19]  Ludger Gerdesmeyer,et al.  Antibacterial effects of extracorporeal shock waves. , 2005, Ultrasound in medicine & biology.

[20]  W. Pitt,et al.  Ultrasound Increases the Rate of Bacterial Cell Growth , 2003, Biotechnology progress.

[21]  W. Ennis,et al.  Ultrasound therapy for recalcitrant diabetic foot ulcers: results of a randomized, double-blind, controlled, multicenter study. , 2005, Ostomy/wound management.

[22]  H. Ramadan,et al.  Effect of Ototopical Medications on Tympanostomy Tube Biofilms , 2007, The Laryngoscope.

[23]  M. Dyson,et al.  The effect of therapeutic ultrasound on angiogenesis. , 1990, Ultrasound in medicine & biology.

[24]  S Meghji,et al.  Ultrasound stimulates nitric oxide and prostaglandin E2 production by human osteoblasts. , 2002, Bone.

[25]  M. Desrosiers,et al.  Effectiveness of Topical Antibiotics on Staphylococcus Aureus Biofilm in Vitro , 2007, American journal of rhinology.

[26]  J. Palmer,et al.  Evaluation of the in vivo efficacy of topical tobramycin against Pseudomonas sinonasal biofilms. , 2007, The Journal of antimicrobial chemotherapy.

[27]  Jun Yuan,et al.  Structure and chemistry of Nb/sapphire interfaces, with and without interlayers of Sb and Cr , 1995 .

[28]  Garth James,et al.  Methods for Removing Bacterial Biofilms: In Vitro Study using Clinical Chronic Rhinosinusitis Specimens , 2007, American journal of rhinology.

[29]  M. Desrosiers,et al.  Treatment of Chronic Rhinosinusitis Refractory to Other Treatments with Topical Antibiotic Therapy Delivered by Means of a Large-Particle Nebulizer: Results of a Controlled Trial , 2001, Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery.

[30]  V. Gupta,et al.  Evaluation of laser spallation as a technique for measurement of cell adhesion strength. , 2007, Journal of biomedical materials research. Part A.

[31]  G. B. Schaalje,et al.  Ultrasonically Enhanced Vancomycin Activity Against Staphylococcus Epidermidis Biofilms in Vivo , 2004, Journal of biomaterials applications.