Biomimetic peptide‐enriched electrospun polymers: A photoelectron and infrared spectroscopy study

Biomimetic polymer nanofibers of poly(e-caprolactone) and poly(L-lactide–caprolactone) copolymer were prepared by electrospinning. Modifications of the polymer nanofibers aimed at improving their biomimetic properties were performed by two different routes: (1) immobilization of an adhesion peptide, which mimicked the adhesion sequence of the extracellular matrix protein fibronectin, on the polymer surface and (2) incorporation of self-complementary oligopeptides, which showed alternated hydrophilic and hydrophobic side chain groups and was capable of generating extended ordered structures by self-assembling, into the polymer nanofibers. The structure of the polymer/peptide nanofibers was investigated by X-ray photoelectron and Fourier transform infrared spectroscopies. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2011

[1]  J. Amédée,et al.  Effect of surface roughness of the titanium alloy Ti-6Al-4V on human bone marrow cell response and on protein adsorption. , 2001, Biomaterials.

[2]  D. Paneva,et al.  Novel electrospun poly(ε-caprolactone)-based bicomponent nanofibers possessing surface enriched in tertiary amino groups , 2008 .

[3]  Myung-Seob Khil,et al.  Characterization of nano-structured poly(ε-caprolactone) nonwoven mats via electrospinning , 2003 .

[4]  N. Manolova,et al.  Electrospun nanofibrous mats containing quaternized chitosan and polylactide with in vitro antitumor activity against HeLa cells. , 2010, Biomacromolecules.

[5]  M. Dettin,et al.  A NEXAFS and XPS study of the adsorption of self‐assembling peptides on TiO2: the influence of the side chains , 2008 .

[6]  A. Lebugle,et al.  Structural characteristics of a globular protein investigated by X-ray photoelectron spectroscopy: comparison between a legumin film and a powdered legumin. , 1995, Biochimica et biophysica acta.

[7]  M. Khil,et al.  In vitro hydrolytic degradation of poly(ɛ-caprolactone) grafted dextran fibers and films , 2008 .

[8]  T. Holmes,et al.  Novel peptide-based biomaterial scaffolds for tissue engineering. , 2002, Trends in biotechnology.

[9]  K. Ishihara,et al.  Preparation of electrospun poly(l-lactide-co-caprolactone-co-glycolide)/phospholipid polymer/rapamycin blended fibers for vascular application , 2009 .

[10]  N. Manolova,et al.  Antitumor activity of quaternized chitosan-based electrospun implants against Graffi myeloid tumor. , 2010, International journal of pharmaceutics.

[11]  S. Ramakrishna,et al.  Coaxial electrospinning of (fluorescein isothiocyanate-conjugated bovine serum albumin)-encapsulated poly(epsilon-caprolactone) nanofibers for sustained release. , 2006, Biomacromolecules.

[12]  S. Monti,et al.  Self Assembling behaviour of self-complementary oligopeptides on biocompatible substrates , 2010 .

[13]  M. Laguerre,et al.  Structure, orientation and affinity for interfaces and lipids of ideally amphipathic lytic LiKj(i=2j) peptides. , 1999, Biochimica et biophysica acta.

[14]  Rosica Mincheva,et al.  Polylactide stereocomplex-based electrospun materials possessing surface with antibacterial and hemostatic properties. , 2010, Biomacromolecules.

[15]  Wen‐Teng Wu,et al.  Electrospun polyacrylonitrile nanofibrous membranes for lipase immobilization , 2007 .

[16]  P. Haris,et al.  The conformational analysis of peptides using fourier transform IR spectroscopy , 1995, Biopolymers.

[17]  M. Dettin,et al.  Assessment of novel chemical strategies for covalent attachment of adhesive peptides to rough titanium surfaces: XPS analysis and biological evaluation. , 2009, Journal of biomedical materials research. Part A.

[18]  N. Manolova,et al.  Electrospun non-woven nanofibrous hybrid mats based on chitosan and PLA for wound-dressing applications. , 2009, Macromolecular bioscience.

[19]  C. Rudd,et al.  Boron trifluoride-catalyzed degradation of poly-ɛ-caprolactone at ambient temperature , 2009 .

[20]  J. Six,et al.  Surface characteristics of PLA and PLGA films , 2006 .

[21]  N. Manolova,et al.  Functionalized electrospun mats from styrene–maleic anhydride copolymers for immobilization of acetylcholinesterase , 2010 .

[22]  Jian Shen,et al.  Fabrication of protein‐doped PLA composite nanofibrous scaffolds for tissue engineering , 2008 .

[23]  C. Grandi,et al.  Covalent surface modification of titanium oxide with different adhesive peptides: surface characterization and osteoblast-like cell adhesion. , 2009, Journal of biomedical materials research. Part A.

[24]  M. Dettin,et al.  Thin films of a self-assembling peptide on TiO2 and Au studied by NEXAFS, XPS and IR spectroscopies , 2006 .

[25]  Byong-Taek Lee,et al.  Electro-spinning of PLGA/PCL blends for tissue engineering and their biocompatibility , 2010, Journal of materials science. Materials in medicine.

[26]  Lisbeth Grøndahl,et al.  Controlled release of heparin from poly(ε-caprolactone) electrospun fibers , 2006 .

[27]  C. Domenici,et al.  Characterisation of blends between poly(ε-caprolactone) and polysaccharides for tissue engineering applications , 2009 .

[28]  A. Rich,et al.  Spontaneous assembly of a self-complementary oligopeptide to form a stable macroscopic membrane. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[29]  Xiaotong Zheng,et al.  Preparation and characterization of a novel electrospun spider silk fibroin/poly(D,L-lactide) composite fiber. , 2008, The journal of physical chemistry. B.