A novel electrochemical sensing system for inosine and its application for inosine determination in pharmaceuticals and human serum

Abstract The voltammetric behaviors of inosine at La(OH) 3 nanowires (LNW) modified carbon paste electrode (LNW/CPE) in the presence of Cu(II) ion and K 2 S 2 O 8 were investigated. The oxidation peak at 0.20 V was attributed to inosine oxidation in the formed inosine–Cu(II) complex, while the reduction peak at −0.20 V was attributed to Cu(II) ion reduction in the complex. The electrochemical oxidation potential of inosine was reduced, and the analytical sensitivity and selectivity was improved. A novel sensing system for monitoring inosine was proposed. The oxidation peak current at 0.20 V was rectilinear with inosine concentration in the range of 4.0 × 10 −9 –2.0 × 10 −8  mol L −1 , with a detection limit of 8.3 × 10 −10  mol L −1 . The proposed system was applied to inosine determination in both pharmaceutical preparations and human serum.

[1]  L. Havran,et al.  Inosine reduction study at low pH by a combination of electrochemical methods , 1998 .

[2]  Csaba Szabó,et al.  Immunomodulatory and neuroprotective effects of inosine. , 2004, Trends in pharmacological sciences.

[3]  C. Brett,et al.  Voltammetric and impedance studies of inosine-5'-monophosphate and hypoxanthine. , 2003, Bioelectrochemistry.

[4]  Qing Peng,et al.  A general strategy for nanocrystal synthesis , 2005, Nature.

[5]  P. Elving,et al.  Analytical Utilization of the Polarographic and Voltammetric Behavior of Purines and Pyrimidines. , 1962 .

[6]  Xun Wang,et al.  Rare-Earth-compound nanowires, nanotubes, and fullerene-like nanoparticles: synthesis, characterization, and properties. , 2003, Chemistry.

[7]  R. Goyal,et al.  Electrochemical oxidation of hypoxanthine , 1981 .

[8]  I. Park,et al.  Simultaneous determination of hypoxanthine, inosine and inosine 5′-monophosphate with serially connected three enzyme reactors , 1999 .

[9]  Y. Li,et al.  Rare Earth Compound Nanotubes , 2003, Advanced Materials.

[10]  M. Gonçalves,et al.  VOLTAMMETRIC STUDIES OF PURINE BASES AND PURINE NUCLEOSIDES WITH COPPER , 1996 .

[11]  M. Khalil,et al.  Binary and ternary complexes of inosine. , 1998, Talanta.

[12]  T. Yao,et al.  Rapid measurement of fish freshness indices by an amperometric flow-injection system with a 16-way switching valve and immobilized enzyme reactors , 2002 .

[13]  L. Mao,et al.  Amperometric on-line sensor for continuous measurement of hypoxanthine based on osmium-polyvinylpyridine gel polymer and xanthine oxidase bienzyme modified glassy carbon electrode , 2000 .

[14]  Jiye Jin,et al.  Amperometric sensor for glucose and hypoxanthine based on a PdIrO(2) modified electrode by a co-crosslinking bienzymic system. , 2002, Talanta.

[15]  Etsuo Watanabe,et al.  Flow system for fish freshness determination based on double multi-enzyme reactor electrodes. , 2002, Biosensors & bioelectronics.