Enzyme-based fiber optic zinc biosensor

A fluoresence-based fiber optic biosensor capable of detecting zinc(II) at nanomolar concentrations is described. The sensor transduces the specific recognition of the ion by an enzyme (carbonic anhydrase) as change in the fluorescence of an inhibitor which binds to the zinc in the active site. The concentration of metal ion is proportional to the ratio of fluorescence intensity at two wavelengths, corresponding to the emission from bound and free inhibitor. Sensing of zinc may be performed through a single optical fiber.

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