Bile ascites or peritonitis in infancy is a rare condition that is usually caused by spontaneous perforation of the extrahepatic tree. Recently, an infant with bile ascites from a spontaneously ruptured choledochal cyst was treated. The use of Disofenin Tc 99m sequential scintiscanning was found to be an effective, rapid, and noninvasive preoperative tool in the demonstration of a bile leak. Disofenin Tc 99m scintiscanning may be the best and most informative method currently available in the preoperative diagnosis of bile ascites.