Molecular diagnostics

Diagnostic molecular biology is arguably the fastest growing area in current laboratory-based medicine. Growth of the so called ‘omics’ technologies has, over the last decade, led to a gradual migration away from the ‘one test, one pathogen’ paradigm, toward multiplex approaches to infectious disease diagnosis, which have led to significant improvements in clinical diagnostics and ultimately improved patient care.

[1]  D. Corcoran,et al.  Comparison of the EntericBio Multiplex PCR System with Routine Culture for Detection of Bacterial Enteric Pathogens , 2009, Journal of Clinical Microbiology.

[2]  B. Paster,et al.  Microarray analysis of the microflora of root caries in elderly , 2009, European Journal of Clinical Microbiology & Infectious Diseases.

[3]  Ruth Ann Luna,et al.  Metagenomic pyrosequencing and microbial identification. , 2009, Clinical chemistry.

[4]  S Falkow,et al.  Molecular Koch's postulates applied to microbial pathogenicity. , 1988, Reviews of infectious diseases.

[5]  David W Ussery,et al.  Characterization of probiotic Escherichia coli isolates with a novel pan-genome microarray , 2007, Genome Biology.

[6]  Shea N Gardner,et al.  A microbial detection array (MDA) for viral and bacterial detection , 2010, BMC Genomics.

[7]  J. Derisi,et al.  Microarray-based detection and genotyping of viral pathogens , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[8]  Michel Eichelbaum,et al.  Pharmacogenomics and individualized drug therapy. , 2006, Annual review of medicine.

[9]  T. Dandekar,et al.  Pharmacogenomic strategies against microbial resistance: from bright to bleak to innovative. , 2010, Pharmacogenomics.

[10]  R. Sleator,et al.  Emerging dynamics of human campylobacteriosis in Southern Ireland. , 2011, FEMS immunology and medical microbiology.

[11]  J. Venter,et al.  Sequence analysis of the human genome: implications for the understanding of nervous system function and disease. , 2001, Archives of neurology.

[12]  S. Socransky,et al.  Comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray. , 2009, The Journal of Periodontology.

[13]  Charles Lee,et al.  Copy number variants and pharmacogenomics. , 2006, Pharmacogenomics.

[14]  J. Schachter.  DFA, EIA, PCR, LCR and other technologies: what tests should be used for diagnosis of chlamydia infections? , 1997, Immunological investigations.

[15]  S. Hu,et al.  The role and impact of SNPs in pharmacogenomics and personalized medicine. , 2011, Current drug metabolism.

[16]  International Human Genome Sequencing Consortium.  Initial sequencing and analysis of the human genome , 2001, Nature.

[17]  Masahira Hattori,et al.  The Human Intestinal Microbiome: A New Frontier of Human Biology , 2009, DNA research : an international journal for rapid publication of reports on genes and genomes.

[18]  W. Coulter,et al.  Development of a novel DNA microarray to detect bacterial pathogens in patients with chronic obstructive pulmonary disease (COPD) , 2010, Journal of Microbiological Methods.

[19]  R. Vossen,et al.  High‐Resolution Melting Analysis (HRMA)—More than just sequence variant screening , 2009, Human mutation.

[20]  T. Derion.  Considerations for the planning and conduct of reproducibility studies of in vitro diagnostic tests for infectious agents. , 2003, Biotechnology annual review.

[21]  F. Tenover.  Potential impact of rapid diagnostic tests on improving antimicrobial use , 2010, Annals of the New York Academy of Sciences.

[22]  P. Williams,et al.  A Functional Gene Array for Detection of Bacterial Virulence Elements , 2007, PloS one.

[23]  Stephen A Bustin,et al.  Reliability of real-time reverse-transcription PCR in clinical diagnostics: gold standard or substandard? , 2009, Expert review of molecular diagnostics.

[24]  A. Szczepura,et al.  Evaluation of molecular techniques in prediction and diagnosis of cytomegalovirus disease in immunocompromised patients. , 2006, Health technology assessment.

[25]  D. Chia,et al.  Variations of oral microbiota are associated with pancreatic diseases including pancreatic cancer , 2011, Gut.

[26]  Dhavendra Kumar,et al.  From evidence-based medicine to genomic medicine , 2007, Genomic Medicine.

[27]  E. Abrahams,et al.  The Case for Personalized Medicine , 2009, Journal of diabetes science and technology.

[28]  Mark J. Pallen,et al.  Bacterial pathogenomics , 2007, Nature.

[29]  S. Bischoff,et al.  'Gut health': a new objective in medicine? , 2011, BMC medicine.

[30]  Roy D Sleator,et al.  The human superorganism - of microbes and men. , 2010, Medical hypotheses.

[31]  C. Knabbe,et al.  Current applications and future trends of molecular diagnostics in clinical bacteriology , 2009, Analytical and bioanalytical chemistry.

[32]  L. Larkin,et al.  Antibiotic resistance: government accepts the recommendations from the ACMSF. Advisory Committee on the Microbiological Safety of Food. , 2000, The Veterinary record.

[33]  A. Witney,et al.  Comparative Phylogenomics of Clostridium difficile Reveals Clade Specificity and Microevolution of Hypervirulent Strains , 2006, Journal of bacteriology.

[34]  G. Procop.  Molecular diagnostics for the detection and characterization of microbial pathogens. , 2007, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[35]  D. Mccormick.  Sequence the Human Genome , 1986, Bio/Technology.

[36]  D. Walker,et al.  Diagnosing emerging and reemerging infectious diseases: the pivotal role of the pathologist. , 2011, Archives of pathology & laboratory medicine.

[37]  N. Woodford,et al.  Molecular detection of antibiotic resistance: when and where? , 2005, The Journal of antimicrobial chemotherapy.

[38]  Jacques Fellay,et al.  Response to antiretroviral treatment in HIV-1-infected individuals with allelic variants of the multidrug resistance transporter 1: a pharmacogenetics study , 2002, The Lancet.

[39]  A. Simor.  Diagnosis, Management, and Prevention of Clostridium difficile Infection in Long‐Term Care Facilities: A Review , 2010, Journal of the American Geriatrics Society.

[40]  R. Rappuoli.  From Pasteur to genomics: progress and challenges in infectious diseases , 2004, Nature Medicine.

[41]  Michael D Cabana,et al.  Role of the gut microbiota in defining human health , 2010, Expert review of anti-infective therapy.

[42]  D. Dykhuizen,et al.  Pathoadaptive mutations: gene loss and variation in bacterial pathogens. , 1999, Trends in microbiology.

[43]  H. McLeod,et al.  The pharmacogenetics of antiretroviral therapy: a review of studies to date. , 2004, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[44]  R. Sleator,et al.  Campylobacter ureolyticus: an emerging gastrointestinal pathogen? , 2011, FEMS immunology and medical microbiology.

[45]  B. Wren,et al.  Comparative phylogenomics of pathogenic bacteria by microarray analysis , 2005, Current Opinion in Microbiology.

[46]  J. Strahilevitz,et al.  High-Resolution Melt Curve Analysis for Identification of Single Nucleotide Mutations in the Quinolone Resistance Gene aac(6′)-Ib-cr , 2010, Antimicrobial Agents and Chemotherapy.

[47]  S. Rhee,et al.  Generation of subspecies level-specific microbial diagnostic microarrays using genes amplified from subtractive suppression hybridization as microarray probes , 2005, Nucleic acids research.

[48]  D. Speers.  Clinical applications of molecular biology for infectious diseases. , 2006, The Clinical biochemist. Reviews.

[49]  J. van Eldere.  Changing needs, opportunities and constraints for the 21st century microbiology laboratory. , 2005, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[50]  Yaoyu E. Wang,et al.  Alterations in diversity of the oral microbiome in pediatric inflammatory bowel disease , 2012, Inflammatory bowel diseases.

[51]  Aaron P. Campbell,et al.  Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[52]  F. Delsuc,et al.  Phylogenomics and the reconstruction of the tree of life , 2005, Nature Reviews Genetics.

[53]  M. Blaser.  Harnessing the power of the human microbiome , 2010, Proceedings of the National Academy of Sciences.

[54]  D. Relman,et al.  Sequence-based identification of microbial pathogens: a reconsideration of Koch's postulates , 1996, Clinical microbiology reviews.

[55]  I. Okeke,et al.  Diagnostics as essential tools for containing antibacterial resistance. , 2011, Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy.