Silencing P2X7 receptor downregulates the expression of TCP-1 involved in lymphoma lymphatic metastasis

P2X7R is an ATP-gated cation channel that participates in cell proliferation and apoptosis. TCP-1 assists with the protein folding. According to our previous research, the P2X7R has a potential role in P388D1 lymphoid neoplasm cells dissemination to peripheral lymph nodes. In order to make a further exploration about the probable mechanism, the lymph nodes which metastasized by P2X7R-silenced P388D1 cells or non-silenced cells were analyzed by 2DE and a MALDI-TOF-based proteomics approach. In the 64 proteins which were differentially expressed between two groups, TCP-1 was found to be significantly decreased in P2X7R shRNA group compared to controls. This correlation was also found in subsequent experiments in vivo and in vitro. The positive correlation between P2X7R and TCP-1 was also proved in both lymphoma and benign lymphadenopathy tissues from patients. It indicates that TCP-1 may be a crucial downstream molecular of P2X7R and plays a novel role in lymphoid neoplasm metastasis.

[1]  Yan Liu,et al.  P2X7 Mediates ATP-Driven Invasiveness in Prostate Cancer Cells , 2014, PloS one.

[2]  F. Malavasi,et al.  Expression of P2X7 ATP Receptor Mediating the IL8 and CCL20 Release in Human Periodontal Ligament Stem Cells , 2014, Journal of cellular biochemistry.

[3]  E. A. Elsayed,et al.  Identification of histone deacetylase 1 protein complexes in liver cancer cells. , 2013, Asian Pacific journal of cancer prevention : APJCP.

[4]  Yueguo Wang,et al.  APRIL Induces Tumorigenesis and Metastasis of Colorectal Cancer Cells via Activation of the PI3K/Akt Pathway , 2013, PloS one.

[5]  R. Aebersold,et al.  Structural Probing of a Protein Phosphatase 2A Network by Chemical Cross-Linking and Mass Spectrometry , 2012, Science.

[6]  G. Schmalzing,et al.  The effect of anions on the human P2X7 receptor. , 2011, Biochimica et biophysica acta.

[7]  T. Binz,et al.  P2X7 Receptors Trigger ATP Exocytosis and Modify Secretory Vesicle Dynamics in Neuroblastoma Cells , 2011, The Journal of Biological Chemistry.

[8]  Yujing Wang,et al.  Targeting P2X7 receptor inhibits the metastasis of murine P388D1 lymphoid neoplasm cells to lymph nodes , 2010, Cell biology international.

[9]  Lin Zhao,et al.  Association of XIAP and P2X7 receptor expression with lymph node metastasis in papillary thyroid carcinoma , 2010, Endocrine.

[10]  Haifeng Jin,et al.  Ribosomal protein L6 promotes growth and cell cycle progression through upregulating cyclin E in gastric cancer cells. , 2010, Biochemical and biophysical research communications.

[11]  Sang Hyuk Lee,et al.  Expression of cell-cycle regulators (cyclin D1, cyclin E, p27kip1, p57kip2) in papillary thyroid carcinoma , 2010, Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery.

[12]  P. Liang,et al.  Intracellular beta-tubulin/chaperonin containing TCP1-beta complex serves as a novel chemotherapeutic target against drug-resistant tumors. , 2009, Cancer research.

[13]  S. Gygi,et al.  p90 Ribosomal S6 Kinase and p70 Ribosomal S6 Kinase Link Phosphorylation of the Eukaryotic Chaperonin Containing TCP-1 to Growth Factor, Insulin, and Nutrient Signaling* , 2009, Journal of Biological Chemistry.

[14]  F. Abdul-Karim,et al.  Activation of P2X7-mediated apoptosis Inhibits DMBA/TPA-induced formation of skin papillomas and cancer in mice , 2009, BMC Cancer.

[15]  Alan Hall,et al.  The cytoskeleton and cancer , 2009, Cancer and Metastasis Reviews.

[16]  M. Gerstein,et al.  Defining the TRiC/CCT interactome links chaperonin function to stabilization of newly-made proteins with complex topologies , 2008, Nature Structural &Molecular Biology.

[17]  J. Bylund,et al.  Serum amyloid A inhibits apoptosis of human neutrophils via a P2X7‐sensitive pathway independent of formyl peptide receptor‐like 1 , 2008, Journal of leukocyte biology.

[18]  Martin Braddock,et al.  Discovery of potent and selective adamantane-based small-molecule P2X(7) receptor antagonists/interleukin-1beta inhibitors. , 2007, Journal of medicinal chemistry.

[19]  G I Murray,et al.  Characterization and over‐expression of chaperonin t‐complex proteins in colorectal cancer , 2006, The Journal of pathology.

[20]  L. Jeong,et al.  Establishment of an assay for P2X7 receptor-mediated cell death. , 2006, Molecules and cells.

[21]  I. Nagaoka,et al.  An Antimicrobial Cathelicidin Peptide, Human CAP18/LL-37, Suppresses Neutrophil Apoptosis via the Activation of Formyl-Peptide Receptor-Like 1 and P2X71 , 2006, The Journal of Immunology.

[22]  Á. Simonyi,et al.  P2X7 nucleotide receptors mediate caspase-8/9/3-dependent apoptosis in rat primary cortical neurons , 2005, Purinergic Signalling.

[23]  D. Adam,et al.  Retraction: Extracellular ATP Induces Cytokine Expression and Apoptosis through P2X7 Receptor in Murine Mast Cells , 2005, The Journal of Immunology.

[24]  Xiao-tong Ma,et al.  Expression of P2X7 in human hematopoietic cell lines and leukemia patients. , 2004, Leukemia research.

[25]  Liqin Wang,et al.  P2X7 receptor-mediated apoptosis of human cervical epithelial cells. , 2004, American journal of physiology. Cell physiology.

[26]  J. Neary,et al.  P2X7 receptors stimulate AKT phosphorylation in astrocytes , 2004, British journal of pharmacology.

[27]  K. Reddy,et al.  Role of MAP kinase in tumor progression and invasion , 2003, Cancer and Metastasis Reviews.

[28]  Yuan-Yi Chang,et al.  Activation of P2X7 purinoceptor-stimulated TGF-beta 1 mRNA expression involves PKC/MAPK signalling pathway in a rat brain-derived type-2 astrocyte cell line, RBA-2. , 2003, Cellular signalling.

[29]  M. Nozaki,et al.  Transcriptional Regulation of the Cytosolic Chaperonin θ Subunit Gene, Cctq, by Ets Domain Transcription Factors Elk-1, Sap-1a, and Net in the Absence of Serum Response Factor* , 2003, Journal of Biological Chemistry.

[30]  J. Neary,et al.  Mechanisms of P2X7 receptor-mediated ERK1/2 phosphorylation in human astrocytoma cells. , 2003, American journal of physiology. Cell physiology.

[31]  W. Kaelin,et al.  Diverse Effects of Mutations in Exon II of the von Hippel-Lindau (VHL) Tumor Suppressor Gene on the Interaction of pVHL with the Cytosolic Chaperonin and pVHL-Dependent Ubiquitin Ligase Activity , 2002, Molecular and Cellular Biology.

[32]  F. Di Virgilio,et al.  P2X7 receptor expression in evolutive and indolent forms of chronic B lymphocytic leukemia. , 2002, Blood.

[33]  Y. Yamaoka,et al.  Increased expression of cytosolic chaperonin CCT in human hepatocellular and colonic carcinoma , 2001, Cell stress & chaperones.

[34]  P. Hogg,et al.  Phosphoglycerate kinase acts in tumour angiogenesis as a disulphide reductase , 2000, Nature.

[35]  V. Thulasiraman,et al.  Formation of the VHL-elongin BC tumor suppressor complex is mediated by the chaperonin TRiC. , 1999, Molecular cell.

[36]  D. Ferrari,et al.  P2X7/P2Z Purinoreceptor-mediated Activation of Transcription Factor NFAT in Microglial Cells* , 1999, The Journal of Biological Chemistry.

[37]  Robert J. Schumacher,et al.  Maturation of Human Cyclin E Requires the Function of Eukaryotic Chaperonin CCT , 1998, Molecular and Cellular Biology.

[38]  F. Di Virgilio,et al.  Extracellular ATP triggers IL-1 beta release by activating the purinergic P2Z receptor of human macrophages. , 1997, Journal of immunology.

[39]  G. Farr,et al.  Chaperonin-Mediated Folding in the Eukaryotic Cytosol Proceeds through Rounds of Release of Native and Nonnative Forms , 1997, Cell.

[40]  M. Yaffe,et al.  The t-complex polypeptide 1 complex is a chaperonin for tubulin and actin in vivo. , 1993, Proceedings of the National Academy of Sciences of the United States of America.