Rapid detection of tenuazonic acid in cereal and fruit juice using a lateral-flow immunochromatographic assay strip

ABSTRACT A lateral-flow immunochromatographic assay (ICA) strip was developed for determination of tenuazonic acid (TeA) using both semi-quantitative and quantitative methods. The ICA strip was based on produced anti-TeA monoclonal antibody, and could be used for TeA analysis in cereal and fruit juice, with visual limits of detection of 1600 ng/g and 400 ng/mL, and cut-off values of 6400 ng/g and 3200 ng/mL, respectively. The calculated limits of detection values were 126.4 ng/g and 65.3 ng/mL for wheat and apple juice samples, respectively. The recovery rates ranged from 90% to 129%. In summary, the developed strip was effective for TeA detection and would be suitable for on-site detection and rapid screening of samples.

[1]  P. Dilkin,et al.  Natural occurrence of tenuazonic acid and Phoma sorghina in Brazilian sorghum grains at different maturity stages. , 2017, Food chemistry.

[2]  Zheng-Jun Xie,et al.  Development of ic-ELISA and lateral-flow immunochromatographic assay strip for the detection of citrinin in cereals , 2017 .

[3]  Zheng-Jun Xie,et al.  Development of indirect competitive ELISA and lateral-flow immunochromatographic assay strip for the detection of sterigmatocystin in cereal products , 2017 .

[4]  Zheng-Jun Xie,et al.  Development of ic-ELISA and lateral-flow immunochromatographic assay strip for the detection of vancomycin in raw milk and animal feed , 2017 .

[5]  H. Mol,et al.  Alternaria toxins and conjugates in selected foods in the Netherlands , 2016 .

[6]  H. Humpf,et al.  Determination of Exposure to the Alternaria Mycotoxin Tenuazonic Acid and Its Isomer allo-Tenuazonic Acid in a German Population by Stable Isotope Dilution HPLC-MS(3). , 2016, Journal of agricultural and food chemistry.

[7]  Liqiang Liu,et al.  Development of ic-ELISA and lateral-flow immunochromatographic assay strip for the detection of folic acid in energy drinks and milk samples , 2016 .

[8]  Xueli Cao,et al.  Determination of Alternaria mycotoxins in wine and juice using ionic liquid modified countercurrent chromatography as a pretreatment method followed by high-performance liquid chromatography. , 2016, Journal of chromatography. A.

[9]  Hua Kuang,et al.  A gold nanoparticle-based semi-quantitative and quantitative ultrasensitive paper sensor for the detection of twenty mycotoxins. , 2016, Nanoscale.

[10]  Liqiang Liu,et al.  Development of an immunochromatographic strip for the semi-quantitative and quantitative detection of biotin in milk and milk products , 2016 .

[11]  Liqiang Liu,et al.  Development of Sensitive, Rapid, and Effective Immunoassays for the Detection of Vitamin B12 in Fortified Food and Nutritional Supplements , 2016, Food Analytical Methods.

[12]  H. Humpf,et al.  Detection and Quantitative Analysis of the Non-cytotoxic allo-Tenuazonic Acid in Tomato Products by Stable Isotope Dilution HPLC-MS/MS. , 2015, Journal of agricultural and food chemistry.

[13]  N. Magan,et al.  Alternaria in Food: Ecophysiology, Mycotoxin Production and Toxicology , 2015, Mycobiology.

[14]  Liqiang Liu,et al.  Development of an anti-chlorothalonil monoclonal antibody based on a novel designed hapten , 2015 .

[15]  Shanshan Song,et al.  Development of an Immunochromatographic Strip for Rapid Detection of Pantoea stewartii subsp. stewartii , 2015, Sensors.

[16]  Feng Xue,et al.  Development of a monoclonal antibody-based immunochromatographic strip for cephalexin , 2015 .

[17]  M. Rychlik,et al.  Development of analytical methods for the determination of tenuazonic acid analogues in food commodities. , 2013, Journal of chromatography. A.

[18]  M. Rychlik,et al.  Potential health hazards due to the occurrence of the mycotoxin tenuazonic acid in infant food , 2013, European Food Research and Technology.

[19]  E. Usleber,et al.  Enzyme immunoassay for tenuazonic acid in apple and tomato products. , 2011, Journal of agricultural and food chemistry.

[20]  R. Dietrich,et al.  Widespread occurrence of low levels of alternariol in apple and tomato products, as determined by comparative immunochemical assessment using monoclonal and polyclonal antibodies. , 2011, Journal of agricultural and food chemistry.

[21]  M. Koch,et al.  Determination of the Alternaria mycotoxin tenuazonic acid in cereals by high-performance liquid chromatography-electrospray ionization ion-trap multistage mass spectrometry after derivatization with 2,4-dinitrophenylhydrazine. , 2009, Journal of chromatography. A.

[22]  V. Ostry,et al.  Alternaria mycotoxins: an overview of chemical characterization, producers, toxicity, analysis and occurrence in foodstuffs , 2008 .

[23]  R. Barkai-golan,et al.  Mouldy fruits and vegetables as a source of mycotoxins: part 1 , 2008 .

[24]  S. Qiang,et al.  Environmental, genetic and cellular toxicity of tenuazonic acid isolated from Alternaira alternata , 2008 .

[25]  Zhou Yu,et al.  [Preparation and identification of a monoclonal antibody against citrinin]. , 2007, Wei sheng yan jiu = Journal of hygiene research.

[26]  M. Agut,et al.  Optimization of the Method of Detection of Metabolites Produced by the Alternaria Genus: Alternariol, Alternariol Monomethyl Ether, Altenuene, Altertoxin I and Tentoxin , 2002 .

[27]  P. Scott,et al.  Gas chromatography-mass spectrometry of Alternaria mycotoxins. , 1997, Journal of chromatography. A.

[28]  E. Ishikawa.  [Enzyme immunoassay]. , 1978, Nihon rinsho. Japanese journal of clinical medicine.